C57BL/6JCya-Krt19em1flox/Cya
Common Name:
Krt19-flox
Product ID:
S-CKO-03306
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Krt19-flox
Strain ID
CKOCMP-16669-Krt19-B6J-VA
Gene Name
Product ID
S-CKO-03306
Gene Alias
CK-19; EndoC; K19; Krt-1.19; Krt1-19
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Krt19em1flox/Cya mice (Catalog S-CKO-03306) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000007317
NCBI RefSeq
NM_008471
Target Region
Exon 2~6
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
Krt19, also known as Keratin 19, is a type I cytokeratin that forms the intermediate filament cytoskeleton and is crucial for maintaining epithelial cell structure [3,4]. It has been associated with various biological processes and disease-related pathways. For example, it is involved in cell-cycle regulation, cell death, and pathways like Wnt/β-catenin, epithelial-mesenchymal transition (EMT), and immune-related processes [2,3,4,5].
In a mouse model, multiplexed genome editing of hepatocytes in vivo demonstrated that Krt19 promoted liver tumorigenesis. Krt19 knockout markedly enhanced histone acetylation levels as it promotes CoREST complex formation, increasing histone deacetylase activity. This indicates its role in liver cancer dedifferentiation through epigenetic regulation [1]. In pancreatic cancer, overexpression of Krt19 boosted cell proliferation, migration, and invasion. Hypoxia-induced upregulation of Krt19, along with loss of miR-642a-5p, favored cancer progression [2]. In thyroid cancer, knockdown of Krt19 inhibited the proliferation and migration of cancer cell lines, suggesting its role in promoting metastasis via EMT [5].
In conclusion, Krt19 plays essential roles in maintaining epithelial cell integrity and is involved in multiple disease-related processes. Gene-knockout studies in mice, especially in liver, pancreatic, and thyroid cancer models, have revealed its significant impact on tumorigenesis, metastasis, and epigenetic regulation, providing valuable insights into the mechanisms of these diseases and potential therapeutic targets.
References:
1. Han, Shixun, Fan, Haonan, Zhong, Guoxuan, Fang, Dong, Zhao, Bin. 2024. Nuclear KRT19 is a transcriptional corepressor promoting histone deacetylation and liver tumorigenesis. In Hepatology (Baltimore, Md.), 81, 808-822. doi:10.1097/HEP.0000000000000875. https://pubmed.ncbi.nlm.nih.gov/38557414/
2. Shi, Hua-Qing, Li, Xin, Chen, Zhou, Zhang, Hui, Zhou, Wen-Ce. 2024. KRT19 is regulated by miR-642a-5p and promotes pancreatic cancer progression through the Wnt/β-catenin pathway. In iScience, 27, 110782. doi:10.1016/j.isci.2024.110782. https://pubmed.ncbi.nlm.nih.gov/39280598/
3. Yuan, Xun, Yi, Ming, Dong, Bing, Chu, Qian, Wu, Kongming. 2021. Prognostic significance of KRT19 in Lung Squamous Cancer. In Journal of Cancer, 12, 1240-1248. doi:10.7150/jca.51179. https://pubmed.ncbi.nlm.nih.gov/33442422/
4. Sun, Zhe, Zhou, Ruijie, Dai, Jinjin, Xu, Yao, Zhang, Tongcun. 2023. KRT19 is a Promising Prognostic Biomarker and Associates with Immune Infiltrates in Serous Ovarian Cystadenocarcinoma. In International journal of general medicine, 16, 4849-4862. doi:10.2147/IJGM.S419235. https://pubmed.ncbi.nlm.nih.gov/37916194/
5. Wang, Xuhong, Xu, Xiaoqin, Peng, Chen, Jing, Jiexian, Zhao, Hongcai. 2019. BRAFV600E-induced KRT19 expression in thyroid cancer promotes lymph node metastasis via EMT. In Oncology letters, 18, 927-935. doi:10.3892/ol.2019.10360. https://pubmed.ncbi.nlm.nih.gov/31289571/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen