C57BL/6JCya-Lamp1em1flox/Cya
Common Name:
Lamp1-flox
Product ID:
S-CKO-03347
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Lamp1-flox
Strain ID
CKOCMP-16783-Lamp1-B6J-VA
Gene Name
Product ID
S-CKO-03347
Gene Alias
CD107a; LGP-120; LGP-A; Lamp-1; P2B; Perk
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Lamp1em1flox/Cya mice (Catalog S-CKO-03347) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000033824
NCBI RefSeq
NM_010684
Target Region
Exon 2
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Lamp1, short for Lysosomal-associated membrane protein 1, is one of the major proteins in the lysosomal membrane. It is often used as a lysosome marker, although not all LAMP1-labeled organelles are true degradative lysosomes [1]. Lamp1 is involved in the endolysosomal system, which is crucial for cellular catabolism, nutrient recycling, and signaling. It also participates in processes like autophagy and lipid transport, and its functions are related to maintaining cellular homeostasis [2].
In Drosophila, Lamp1 is dispensable for viability and autophagy, but it is involved in lipid metabolism as its deficiency leads to defects in lipid transport, with elevated levels of sterols and diacylglycerols [2]. In Drosophila models of Parkinson's disease, lack of Lamp1 increases susceptibility to paraquat (a pro-oxidant) and exacerbates locomotor defects induced by mutant α-synuclein, suggesting a role in protecting against oxidative stress and α-synuclein neurotoxicity [4]. In mice, while single deficiency in Lamp1 is viable, double deficiency of Lamp1 and Lamp2 is embryonic lethal, indicating shared functions, and Lamp2 single-deficiency has more severe consequences [3].
In conclusion, Lamp1 has diverse functions in cells, playing important roles in lipid transport, protection against oxidative stress and neurotoxicity in neurodegenerative disease models. Gene-knockout studies in Drosophila and mice have been valuable in revealing these functions, contributing to our understanding of biological processes and disease mechanisms related to the endolysosomal system.
References:
1. Cheng, Xiu-Tang, Xie, Yu-Xiang, Zhou, Bing, Farfel-Becker, Tamar, Sheng, Zu-Hang. 2018. Revisiting LAMP1 as a marker for degradative autophagy-lysosomal organelles in the nervous system. In Autophagy, 14, 1472-1474. doi:10.1080/15548627.2018.1482147. https://pubmed.ncbi.nlm.nih.gov/29940787/
2. Chaudhry, Norin, Sica, Margaux, Surabhi, Satya, MacIntosh, Gustavo C, Jenny, Andreas. 2022. Lamp1 mediates lipid transport, but is dispensable for autophagy in Drosophila. In Autophagy, 18, 2443-2458. doi:10.1080/15548627.2022.2038999. https://pubmed.ncbi.nlm.nih.gov/35266854/
3. Eskelinen, Eeva-Liisa. 2006. Roles of LAMP-1 and LAMP-2 in lysosome biogenesis and autophagy. In Molecular aspects of medicine, 27, 495-502. doi:. https://pubmed.ncbi.nlm.nih.gov/16973206/
4. Rahmani, Zohra, Surabhi, Satya, Rojo-Cortés, Francisca, Jenny, Andreas, Birman, Serge. 2022. Lamp1 Deficiency Enhances Sensitivity to α-Synuclein and Oxidative Stress in Drosophila Models of Parkinson Disease. In International journal of molecular sciences, 23, . doi:10.3390/ijms232113078. https://pubmed.ncbi.nlm.nih.gov/36361864/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen