C57BL/6JCya-Lcn2em1flox/Cya
Common Name:
Lcn2-flox
Product ID:
S-CKO-03363
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Lcn2-flox
Strain ID
CKOCMP-16819-Lcn2-B6J-VA
Gene Name
Product ID
S-CKO-03363
Gene Alias
24p3; NRL; Sip24
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Lcn2em1flox/Cya mice (Catalog S-CKO-03363) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000050785
NCBI RefSeq
NM_008491
Target Region
Exon 2
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Lcn2, also known as lipocalin 2, is a secreted glycoprotein produced by immune cells like neutrophils and macrophages. It has diverse functions including transporting hydrophobic ligands across cell membranes, regulating immune responses, maintaining iron balance, and promoting epithelial cell differentiation. Lcn2 is involved in multiple physiological processes and its expression is up-regulated in various human diseases and cancers [2].
In cancer cachexia, studies using mouse models have provided valuable insights. In lung cancer cachexia, LCN2 secreted by tissue-infiltrating neutrophils induces ferroptosis and wasting of adipose and muscle tissues. Inhibition of LCN2 expression significantly reduces cachexia symptoms and tissue wasting [1]. In pancreatic cancer cachexia, Lcn2-knockout mice show that Lcn2 mediates adipocyte-muscle-tumor communication and hypothermia. Therapeutic suppression of Lcn2 may minimize cachexia progression [3]. In sepsis-induced liver injury, LCN2 depletion in mice exacerbates liver injury, oxidative stress, and ferroptosis, while LCN2 overexpression ameliorates LPS-induced cell injury in an in vitro sepsis model [4].
In conclusion, Lcn2 is a multifunctional protein involved in various biological processes. Model-based research, especially using Lcn2 KO/CKO mouse models, has revealed its crucial roles in cancer cachexia and sepsis-induced liver injury. These findings contribute to understanding the mechanisms of these diseases and potentially developing new therapeutic strategies targeting Lcn2.
References:
1. Wang, Dong, Li, Xiaohui, Jiao, Defeng, Zheng, Xiaohu, Wei, Haiming. 2023. LCN2 secreted by tissue-infiltrating neutrophils induces the ferroptosis and wasting of adipose and muscle tissues in lung cancer cachexia. In Journal of hematology & oncology, 16, 30. doi:10.1186/s13045-023-01429-1. https://pubmed.ncbi.nlm.nih.gov/36973755/
2. Bao, Yuxiang, Yan, Zhongliang, Shi, Nianmei, Cheng, Xiaoming, Lv, Junyuan. 2024. LCN2: Versatile players in breast cancer. In Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 171, 116091. doi:10.1016/j.biopha.2023.116091. https://pubmed.ncbi.nlm.nih.gov/38171248/
3. Lemecha, Mengistu, Chalise, Jaya Prakash, Takamuku, Yuki, Larson, Garrett, Itakura, Keiichi. 2022. Lcn2 mediates adipocyte-muscle-tumor communication and hypothermia in pancreatic cancer cachexia. In Molecular metabolism, 66, 101612. doi:10.1016/j.molmet.2022.101612. https://pubmed.ncbi.nlm.nih.gov/36243318/
4. Jiang, Yun, Jiang, Zhi-Tian, Zhao, Gang, Wang, Qian, Ling, Qi-Hua. 2024. LCN2 depletion aggravates sepsis-induced liver injury by regulating PTGS2-dependent ferroptosis. In International journal of medical sciences, 21, 2770-2780. doi:10.7150/ijms.98246. https://pubmed.ncbi.nlm.nih.gov/39512683/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen