C57BL/6JCya-Ldhbem1flox/Cya
Common Name:
Ldhb-flox
Product ID:
S-CKO-03370
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Ldhb-flox
Strain ID
CKOCMP-16832-Ldhb-B6J-VA
Gene Name
Product ID
S-CKO-03370
Gene Alias
H-Ldh; LDH-B; LDH-H; Ldh-2; Ldh2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ldhbem1flox/Cya mice (Catalog S-CKO-03370) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000032373
NCBI RefSeq
NM_008492
Target Region
Exon 3
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Ldhb, lactate dehydrogenase B, is a key enzyme in the glycolysis pathway. It reversibly catalyzes the conversion between pyruvate and lactate, with a higher affinity for lactate to convert it to pyruvate when oxygen is abundant [2]. This function is crucial for maintaining cellular energy metabolism and metabolite balance.
Ldhb has diverse effects on different biological processes and diseases. In classical swine fever virus (CSFV) infection, knockdown of Ldhb via interfering RNA induced mitochondrial fission and mitophagy, promoted NFKB signaling, inhibited apoptosis, and facilitated CSFV growth, while its overexpression decreased CSFV replication [1]. In leukemia, R-2-hydroxyglutarate attenuated aerobic glycolysis by suppressing the expression of Ldhb, among other genes [3]. In acetaminophen-induced liver injury, decreased SIRT1/PGC-1α/Ldhb expression was observed, and PGC-1α upregulation increased Ldhb synthesis, suggesting the PGC-1α/Ldhb axis as a potential therapeutic target [4]. In human pancreatic β cells, Ldhb limits lactate generation to maintain appropriate insulin release [5]. In hepatocellular carcinoma, DNMT3A-mediated epigenetic silencing of Ldhb contributed to HCC progression by remodeling the tumor immune microenvironment, and Ldhb may be a prognostic biomarker and therapeutic target [6]. In breast cancer, decreased Ldhb expression in tumor cells caused NK cell activation, and Ldhb is a promising target for activating the tumor immune microenvironment [7]. In ovarian cancer, Ldhb regulated histone lactylation on the PD-L1 promoter to promote its expression and immune escape [8]. In ischemic stroke, Ldhb-deficient mice showed increased neuronal survival due to elevated lactate-induced vasodilation [9]. In neurodegeneration, Ldhb deficiency in mice promoted mitochondrial dysfunction-mediated oxidative stress and neurodegeneration, and osmotin treatment could alleviate these effects [10].
In conclusion, Ldhb is essential for maintaining normal cellular metabolism and is involved in various disease processes such as viral infections, cancers, liver injury, ischemic stroke, and neurodegeneration. Gene knockout or knockdown models of Ldhb have been crucial in revealing its functions in these biological processes and disease conditions, providing potential therapeutic targets and insights into disease mechanisms.
References:
1. Fan, Shuangqi, Wu, Keke, Zhao, Mingqiu, Yi, Lin, Chen, Jinding. 2020. LDHB inhibition induces mitophagy and facilitates the progression of CSFV infection. In Autophagy, 17, 2305-2324. doi:10.1080/15548627.2020.1823123. https://pubmed.ncbi.nlm.nih.gov/32924761/
2. Urbańska, Kaja, Orzechowski, Arkadiusz. 2019. Unappreciated Role of LDHA and LDHB to Control Apoptosis and Autophagy in Tumor Cells. In International journal of molecular sciences, 20, . doi:10.3390/ijms20092085. https://pubmed.ncbi.nlm.nih.gov/31035592/
3. Qing, Ying, Dong, Lei, Gao, Lei, Su, Rui, Chen, Jianjun. . R-2-hydroxyglutarate attenuates aerobic glycolysis in leukemia by targeting the FTO/m6A/PFKP/LDHB axis. In Molecular cell, 81, 922-939.e9. doi:10.1016/j.molcel.2020.12.026. https://pubmed.ncbi.nlm.nih.gov/33434505/
4. Hong, Weilong, Zeng, Xue, Wang, Houping, Huang, He, Duan, Chenyang. 2024. PGC-1α loss promotes mitochondrial protein lactylation in acetaminophen-induced liver injury via the LDHB-lactate axis. In Pharmacological research, 205, 107228. doi:10.1016/j.phrs.2024.107228. https://pubmed.ncbi.nlm.nih.gov/38810904/
5. Cuozzo, Federica, Viloria, Katrina, Shilleh, Ali H, Ludwig, Christian, Hodson, David J. 2024. LDHB contributes to the regulation of lactate levels and basal insulin secretion in human pancreatic β cells. In Cell reports, 43, 114047. doi:10.1016/j.celrep.2024.114047. https://pubmed.ncbi.nlm.nih.gov/38607916/
6. Zhang, Peng, Wan, Yi, Ma, Jinrong, Tan, Youwen, Zhang, Chengsheng. 2024. Epigenetic silencing of LDHB promotes hepatocellular carcinoma by remodeling the tumor microenvironment. In Cancer immunology, immunotherapy : CII, 73, 127. doi:10.1007/s00262-024-03717-2. https://pubmed.ncbi.nlm.nih.gov/38739169/
7. Luo, Zhihong, Huang, Xiaohua, Xu, Xinyi, Gong, Ke, Li, Wenhua. . Decreased LDHB expression in breast tumor cells causes NK cell activation and promotes tumor progression. In Cancer biology & medicine, 21, 513-40. doi:10.20892/j.issn.2095-3941.2023.0382. https://pubmed.ncbi.nlm.nih.gov/38525901/
8. Hu, Xuemei, Huang, Zhenqiang, Li, Lingyun. 2024. LDHB Mediates Histone Lactylation to Activate PD-L1 and Promote Ovarian Cancer Immune Escape. In Cancer investigation, 43, 70-79. doi:10.1080/07357907.2024.2430283. https://pubmed.ncbi.nlm.nih.gov/39587817/
9. Lee, Jin Soo, Yoon, Bok Seon, Kim, Yihyang, Park, Chan Bae. 2024. LDHB-deficient brain exhibits resistance to ischemic neuronal cell death due to increased vasodilation. In Biochemical and biophysical research communications, 734, 150766. doi:10.1016/j.bbrc.2024.150766. https://pubmed.ncbi.nlm.nih.gov/39368368/
10. Park, Jun Sung, Saeed, Kamran, Jo, Myeung Hoon, Lee, Gwang, Kim, Myeong Ok. 2022. LDHB Deficiency Promotes Mitochondrial Dysfunction Mediated Oxidative Stress and Neurodegeneration in Adult Mouse Brain. In Antioxidants (Basel, Switzerland), 11, . doi:10.3390/antiox11020261. https://pubmed.ncbi.nlm.nih.gov/35204143/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen