C57BL/6JCya-Lmnb1em1flox/Cya
Common Name:
Lmnb1-flox
Product ID:
S-CKO-03407
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Lmnb1-flox
Strain ID
CKOCMP-16906-Lmnb1-B6J-VA
Gene Name
Product ID
S-CKO-03407
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
18
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Lmnb1em1flox/Cya mice (Catalog S-CKO-03407) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000025486
NCBI RefSeq
NM_010721
Target Region
Exon 2
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Lmnb1, also known as Lamin B1, is a key component of the nuclear skeleton structure. It is involved in various biological processes and may play roles in multiple signaling pathways, though the specific pathways are still being explored. Genetic models, such as gene knockout (KO) and conditional knockout (CKO) mouse models, can be valuable tools to study its functions.
In cancer research, studies on various cancers have shown significant associations. In hepatocellular carcinoma (HCC), Lmnb1 is overexpressed, correlates with pathological stage and poor prognosis, and promotes HCC proliferation in vitro and in vivo by regulating CDKN1A expression [1]. In prostate cancer, while inconsistent results exist, it may play a role in early progression steps [2]. In ovarian cancer, overexpression indicates OC progression, and knockdown inhibits OC cell proliferation and migration by suppressing the FGF1-mediated PI3K-Akt signaling pathway [3]. In lung adenocarcinoma, it is upregulated, correlated with pathological stage and lymphatic metastasis, and its knockdown inhibits tumor development [4]. In addition, in lung cancer, Lmnb1 can activate GPR84 through the JAK-STAT pathway to mediate M2 macrophage polarization [5].
In conclusion, Lmnb1 is crucial for the nuclear skeleton structure and is involved in cancer-related biological processes. Model-based research, especially KO/CKO mouse models, has revealed its role in promoting the progression of multiple cancers, providing potential therapeutic targets and prognostic biomarkers for these diseases.
References:
1. Gao, Dute, Guo, Huahu, Liu, Zhaochen, Jiang, Binghua, Dang, Xiaowei. 2024. LMNB1/CDKN1A Signaling Regulates the Cell Cycle and Promotes Hepatocellular Carcinoma Progression. In Current cancer drug targets, , . doi:10.2174/0115680096299107240427073527. https://pubmed.ncbi.nlm.nih.gov/38778606/
2. Hong, Jian-Hua, Liang, Sung-Tzu, Wang, Alexander Sheng-Shin, Chen, Chung-Hsin, Pu, Yeong-Shiau. 2022. LMNB1, a potential marker for early prostate cancer progression. In American journal of cancer research, 12, 3390-3404. doi:. https://pubmed.ncbi.nlm.nih.gov/35968338/
3. Dong, Jian, Ru, Yi, Zhai, Lianghao, Chen, Biliang, Lv, Xiaohui. 2023. LMNB1 deletion in ovarian cancer inhibits the proliferation and metastasis of tumor cells through PI3K/Akt pathway. In Experimental cell research, 426, 113573. doi:10.1016/j.yexcr.2023.113573. https://pubmed.ncbi.nlm.nih.gov/37003558/
4. Tang, Dan, Luo, Haihai, Xie, An, Zhang, Wei, Xu, Xinping. 2021. Silencing LMNB1 Contributes to the Suppression of Lung Adenocarcinoma Development. In Cancer management and research, 13, 2633-2642. doi:10.2147/CMAR.S275874. https://pubmed.ncbi.nlm.nih.gov/33776481/
5. Ji, Yuanyuan, Wang, Yuekun, Zhang, Ning, Wang, Weijie, Li, Junkuo. 2024. Mechanism of LMNB1 activating GPR84 through JAK-STAT pathway to mediate M2 macrophage polarization in lung cancer. In Human immunology, 85, 111150. doi:10.1016/j.humimm.2024.111150. https://pubmed.ncbi.nlm.nih.gov/39357468/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen