C57BL/6JCya-Rbm39em1flox/Cya
Common Name:
Rbm39-flox
Product ID:
S-CKO-03525
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Rbm39-flox
Strain ID
CKOCMP-170791-Rbm39-B6J-VA
Gene Name
Product ID
S-CKO-03525
Gene Alias
1500012C14Rik; 2310040E03Rik; B330012G18Rik; Rnpc2; caper
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rbm39em1flox/Cya mice (Catalog S-CKO-03525) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000109587
NCBI RefSeq
NM_133242
Target Region
Exon 7
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Rbm39, or RNA-binding motif protein 39, is an RNA-binding protein involved in transcriptional co-regulation and alternative RNA splicing. It has been associated with multiple biological processes and is emerging as a significant factor in cancer-related pathways [1].
Loss-of-function experiments have demonstrated its importance in various cancer types. For example, depletion of Rbm39 in cholangiocarcinoma cells using Nuclease technology or shRNA inhibited cell proliferation in vitro and tumor growth in mice, and revealed a novel Rbm39-EZH2-β-catenin signaling axis crucial for CCA growth [2]. In T-cell acute lymphoblastic leukemia, knockdown of Rbm39 restricted cell proliferation both in vitro and in vivo, and indisulam, which induces Rbm39 degradation, caused widespread aberrant splicing of downstream effector proteins like THOC1 [3]. In osteosarcoma, pharmacological depletion of Rbm39 by aryl sulfonamide (E7820) repressed tumor growth and sensitized cells to cisplatin treatment [4].
In conclusion, Rbm39 is a key protein in RNA-related regulatory processes. Model-based research, especially loss-of-function experiments in cancer models, has shown its significant role in promoting tumor growth and metastasis in multiple cancer types, highlighting its potential as a therapeutic target.
References:
1. Xu, Yuewei, Nijhuis, Anke, Keun, Hector C. 2021. RNA-binding motif protein 39 (RBM39): An emerging cancer target. In British journal of pharmacology, 179, 2795-2812. doi:10.1111/bph.15331. https://pubmed.ncbi.nlm.nih.gov/33238031/
2. Liu, Nianli, Zhang, Jinqiang, Chen, Weina, Ma, Wenbo, Wu, Tong. 2024. RBM39 Enhances Cholangiocarcinoma Growth Through EZH2-mediated WNT7B/β-catenin Pathway. In Cellular and molecular gastroenterology and hepatology, 19, 101404. doi:10.1016/j.jcmgh.2024.101404. https://pubmed.ncbi.nlm.nih.gov/39278404/
3. Ji, Tongting, Yang, Yang, Yu, Juanjuan, Pan, Jian, Lu, Jun. 2024. Targeting RBM39 through indisulam induced mis-splicing of mRNA to exert anti-cancer effects in T-cell acute lymphoblastic leukemia. In Journal of experimental & clinical cancer research : CR, 43, 205. doi:10.1186/s13046-024-03130-8. https://pubmed.ncbi.nlm.nih.gov/39044280/
4. Wang, Jingxuan, Zheng, Lisi, Chen, Wanqi, Kang, Tiebang, Zhong, Li. 2024. Targeting RBM39 suppresses tumor growth and sensitizes osteosarcoma cells to cisplatin. In Oncogene, 44, 575-586. doi:10.1038/s41388-024-03242-7. https://pubmed.ncbi.nlm.nih.gov/39633066/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen