C57BL/6JCya-Madcam1em1flox/Cya
Common Name:
Madcam1-flox
Product ID:
S-CKO-03576
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Madcam1-flox
Strain ID
CKOCMP-17123-Madcam1-B6J-VA
Gene Name
Product ID
S-CKO-03576
Gene Alias
MAdCAM-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Madcam1em1flox/Cya mice (Catalog S-CKO-03576) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000020554
NCBI RefSeq
NM_013591
Target Region
Exon 1~4
Size of Effective Region
~3.0 kb
Detailed Document
Overview of Gene Research
Madcam1, also known as mucosal addressin cell adhesion molecule 1, is a crucial adhesion molecule. It plays a vital role in lymphocyte homing, especially in the gut-associated lymphoid tissue, by interacting with its receptor α4β7 integrin. This interaction is involved in regulating immunity and inflammation processes. The NKX-COUP-TFII morphogenetic code has been identified to direct its expression [6].
In various disease models, Madcam1 has shown significant effects. In cancer, post-antibiotics gut recolonization by Enterocloster species down-regulates ileum Madcam1, leading to the emigration of enterotropic α4β7+CD4+ regulatory T 17 cells into the tumor, indicating its role in cancer immunosurveillance [1]. In colitis, optogenetic activation of local colonic sympathetic innervations reduces endothelial MAdCAM-1 expression, attenuating experimental colitis and immune cell extravasation [2]. In acute liver failure, MAdCAM1 levels are induced and positively correlated with liver damage; activating the cholinergic anti-inflammatory pathway down-regulates MAdCAM1, alleviating liver injury [3]. In glaucoma, MAdCAM-1 is induced in retinal endothelial cells by elevated intraocular pressure, and its neutralization ameliorates retinal ganglion cell loss [4]. In Crohn's disease, treatment with anti-TNF-α reduces the number of MAdCAM-1-positive mucosal capillaries [5]. In checkpoint inhibitor colitis, endothelial cells upregulate MAdCAM-1, which may respond to vedolizumab [7].
In conclusion, Madcam1 is essential for lymphocyte homing and immune-related processes. Studies using gene-related models, such as those in cancer, colitis, liver failure, glaucoma, Crohn's disease, and checkpoint inhibitor colitis, have revealed its role in disease-associated inflammation, immune cell trafficking, and disease progression. Understanding Madcam1 can potentially provide new therapeutic targets for these diseases.
References:
1. Fidelle, Marine, Rauber, Conrad, Alves Costa Silva, Carolina, Kroemer, Guido, Zitvogel, Laurence. 2023. A microbiota-modulated checkpoint directs immunosuppressive intestinal T cells into cancers. In Science (New York, N.Y.), 380, eabo2296. doi:10.1126/science.abo2296. https://pubmed.ncbi.nlm.nih.gov/37289890/
2. Schiller, Maya, Azulay-Debby, Hilla, Boshnak, Nadia, Hakim, Fahed, Rolls, Asya. 2021. Optogenetic activation of local colonic sympathetic innervations attenuates colitis by limiting immune cell extravasation. In Immunity, 54, 1022-1036.e8. doi:10.1016/j.immuni.2021.04.007. https://pubmed.ncbi.nlm.nih.gov/33932356/
3. Fu, Shan, Ni, TianZhi, Zhang, MengMeng, Zhao, YingRen, Liu, JinFeng. 2023. Cholinergic Anti-inflammatory Pathway Attenuates Acute Liver Failure Through Inhibiting MAdCAM1/α4β7-mediated Gut-derived Proinflammatory Lymphocytes Accumulation. In Cellular and molecular gastroenterology and hepatology, 17, 199-217. doi:10.1016/j.jcmgh.2023.10.012. https://pubmed.ncbi.nlm.nih.gov/37926366/
4. He, Chong, Xiu, Wenbo, Chen, Qinyuan, Yu, Ling, Lu, Fang. 2023. Gut-licensed β7+ CD4+ T cells contribute to progressive retinal ganglion cell damage in glaucoma. In Science translational medicine, 15, eadg1656. doi:10.1126/scitranslmed.adg1656. https://pubmed.ncbi.nlm.nih.gov/37531415/
5. Younes, Mamoun, DuPont, Andrew W, Cash, Brooks D, Ertan, Atilla. . Alterations in MAdCAM1-Positive Mucosal Capillaries and Integrin a4b7-Positive Lymphocytes in Crohn's Disease Treated with Anti-TNFα Biologics. In Annals of clinical and laboratory science, 51, 678-685. doi:. https://pubmed.ncbi.nlm.nih.gov/34686510/
6. Dinh, Thanh Theresa, Xiang, Menglan, Rajaraman, Anusha, Pan, Junliang, Butcher, Eugene C. 2022. An NKX-COUP-TFII morphogenetic code directs mucosal endothelial addressin expression. In Nature communications, 13, 7448. doi:10.1038/s41467-022-34991-2. https://pubmed.ncbi.nlm.nih.gov/36460642/
7. He, Jun Yan, Kim, Yang-Joon, Mennillo, Elvira, Kattah, Michael G, Oh, David Y. 2024. Dysregulation of CD4+ and CD8+ resident memory T, myeloid, and stromal cells in steroid-experienced, checkpoint inhibitor colitis. In Journal for immunotherapy of cancer, 12, . doi:10.1136/jitc-2023-008628. https://pubmed.ncbi.nlm.nih.gov/38642938/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen