C57BL/6JCya-Mcl1em1flox/Cya
Common Name:
Mcl1-flox
Product ID:
S-CKO-03678
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Mcl1-flox
Strain ID
CKOCMP-17210-Mcl1-B6J-VA
Gene Name
Product ID
S-CKO-03678
Gene Alias
Gm52627; Mcl-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mcl1em1flox/Cya mice (Catalog S-CKO-03678) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000037947
NCBI RefSeq
NM_008562
Target Region
Exon 1~2
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Mcl1, short for myeloid cell leukemia 1, is an anti-apoptotic protein belonging to the BCL2 family. It plays a crucial role in regulating cell survival, with its dysregulation involved in many physiological and pathological processes such as tumor drug resistance, tissue homeostasis, and programmed cell death [3]. It is commonly expressed in hematologic cancers and is also associated with pathways like mitochondrial oxidative phosphorylation, which impacts processes such as the maintenance of cancer stem cells [1,2].
In various cancers, Mcl1 has been shown to have significant effects. In triple-negative breast cancer (TNBC), it cooperates with MYC to promote chemotherapy-resistant cancer stem cells through regulation of mitochondrial oxidative phosphorylation [2]. High Mcl1 expression contributes to chemotherapy resistance and poor prognosis in TNBC, and a 4-member gene signature has been identified to predict Mcl1 inhibitor sensitivity [6]. In acute myeloid leukemia (AML), Mcl1 directly binds to Hexokinase 2 on the outer mitochondrial membrane, regulating cell metabolism, and high levels of certain metabolic features along with Mcl1 are linked to shorter overall survival [4]. In colorectal carcinoma, chemotherapy-induced Mcl1 nuclear translocation confers chemoresistance, while also exposing a Bcl-xL co-dependency [5].
In conclusion, Mcl1 is a key anti-apoptotic protein with its dysregulation being closely associated with cancer development and chemoresistance, especially in TNBC, AML, and colorectal carcinoma. Understanding Mcl1's functions through various in vivo studies helps in developing targeted therapeutic strategies for these diseases.
References:
1. Roberts, Andrew W, Wei, Andrew H, Huang, David C S. . BCL2 and MCL1 inhibitors for hematologic malignancies. In Blood, 138, 1120-1136. doi:10.1182/blood.2020006785. https://pubmed.ncbi.nlm.nih.gov/34320168/
2. Lee, Kyung-Min, Giltnane, Jennifer M, Balko, Justin M, Fesik, Stephen W, Arteaga, Carlos L. . MYC and MCL1 Cooperatively Promote Chemotherapy-Resistant Breast Cancer Stem Cells via Regulation of Mitochondrial Oxidative Phosphorylation. In Cell metabolism, 26, 633-647.e7. doi:10.1016/j.cmet.2017.09.009. https://pubmed.ncbi.nlm.nih.gov/28978427/
3. Li, Shujing, Guo, Wanping, Wu, Huijian. 2021. The role of post-translational modifications in the regulation of MCL1. In Cellular signalling, 81, 109933. doi:10.1016/j.cellsig.2021.109933. https://pubmed.ncbi.nlm.nih.gov/33508399/
4. Catalano, Gianfranco, Zaza, Alessandra, Banella, Cristina, Voso, Maria Teresa, Noguera, Nelida Ines. 2023. MCL1 regulates AML cells metabolism via direct interaction with HK2. Metabolic signature at onset predicts overall survival in AMLs' patients. In Leukemia, 37, 1600-1610. doi:10.1038/s41375-023-01946-5. https://pubmed.ncbi.nlm.nih.gov/37349598/
5. Fu, Dechen, Pfannenstiel, Luke, Demelash, Abeba, Li, Xiaoxia, Gastman, Brian. 2022. MCL1 nuclear translocation induces chemoresistance in colorectal carcinoma. In Cell death & disease, 13, 63. doi:10.1038/s41419-021-04334-y. https://pubmed.ncbi.nlm.nih.gov/35042842/
6. Duan, Lei, Tadi, Mehrdad Jafari, O'Hara, Kelsey M, Maki, Carl G. 2024. Novel markers of MCL1 inhibitor sensitivity in triple-negative breast cancer cells. In The Journal of biological chemistry, 300, 107375. doi:10.1016/j.jbc.2024.107375. https://pubmed.ncbi.nlm.nih.gov/38762181/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen