C57BL/6JCya-Mcm5em1flox/Cya
Common Name:
Mcm5-flox
Product ID:
S-CKO-03682
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Mcm5-flox
Strain ID
CKOCMP-17218-Mcm5-B6J-VA
Gene Name
Product ID
S-CKO-03682
Gene Alias
Cdc46; Mcmd5; P1-CDC46
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mcm5em1flox/Cya mice (Catalog S-CKO-03682) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000164309
NCBI RefSeq
NM_008566
Target Region
Exon 5
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Mcm5, or minichromosome maintenance complex component 5, is a DNA licensing factor involved in cell proliferation [3]. It is also associated with cell cycle regulation, playing a well-known role in DNA replication [5]. In addition, it has been implicated in multiple signaling pathways, which are crucial for various biological processes such as organogenesis and tumor metastasis [3,5]. Genetic models, especially knockout models, can be valuable for further exploring its functions.
Mcm5 has been extensively studied in cancer research. In lung adenocarcinoma (LUAD), the m6A reader IGF2BP3 recognizes m6A-modified Mcm5 mRNAs, prolonging their stability, upregulating Mcm5 protein. This in turn competitively inhibits SIRT1-mediated deacetylation of Notch1 intracellular domain, promoting partial EMT and metastasis [1]. In colorectal cancer, DDX21, a highly expressed RNA binding protein, phase-separates and binds to the Mcm5 gene locus, and MCM5 is a key downstream target for CRC metastasis [2]. In glioblastoma, increased MCM5 expression promotes cell proliferation and resistance to TMZ [3]. In acute myeloid leukemia, elevated MCM5 levels predict a worse prognosis, and it promotes AML progression by accelerating the cell cycle and DNA replication [4]. In colon adenocarcinoma, MCM5 is an oncogene that promotes disease progression via cell cycle control [6]. In renal cell carcinoma, MCM5 overexpression is associated with a malignant status and poor prognosis, and it plays an important role in cell proliferation [7].
In conclusion, Mcm5 is essential for cell proliferation and DNA replication. Through model-based research, especially in cancer-related studies, it has been shown to be a crucial factor in the progression and prognosis of multiple cancers, including LUAD, colorectal cancer, glioblastoma, AML, colon adenocarcinoma, and renal cell carcinoma. Understanding Mcm5 functions provides potential therapeutic targets for these diseases.
References:
1. Yang, Xia, Bai, Qiaorui, Chen, Weizhong, Yang, Yi, Cai, Junchao. 2023. m6 A-Dependent Modulation via IGF2BP3/MCM5/Notch Axis Promotes Partial EMT and LUAD Metastasis. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 10, e2206744. doi:10.1002/advs.202206744. https://pubmed.ncbi.nlm.nih.gov/37171793/
2. Gao, Huabin, Wei, Huiting, Yang, Yang, Wang, Jia, Han, Anjia. 2023. Phase separation of DDX21 promotes colorectal cancer metastasis via MCM5-dependent EMT pathway. In Oncogene, 42, 1704-1715. doi:10.1038/s41388-023-02687-6. https://pubmed.ncbi.nlm.nih.gov/37029300/
3. Zhou, Jian, Zheng, Housheng, Zhang, Huiru, Ye, Liang, Wang, Lu. 2024. MCM5 is a Novel Therapeutic Target for Glioblastoma. In OncoTargets and therapy, 17, 371-381. doi:10.2147/OTT.S457600. https://pubmed.ncbi.nlm.nih.gov/38765057/
4. Wang, Shuming, Wu, Weiqin, Han, Xiang. 2022. Enhanced MCM5 Level Predicts Bad Prognosis in Acute Myeloid Leukemia. In Molecular biotechnology, 65, 1242-1252. doi:10.1007/s12033-022-00623-9. https://pubmed.ncbi.nlm.nih.gov/36479666/
5. Zhang, Yu, Xia, Jiamin, Liu, Min, Su, Bingyin, Huang, Sizhou. 2022. Mcm5 Represses Endodermal Migration through Cxcr4a-itgb1b Cascade Instead of Cell Cycle Control. In Biomolecules, 12, . doi:10.3390/biom12020286. https://pubmed.ncbi.nlm.nih.gov/35204787/
6. Mao, Jiayan, Shen, Jian, Lu, Xuemei, Wu, Yuan, Chen, Wei. 2023. MCM5 is an oncogene of colon adenocarcinoma and promotes progression through cell cycle control. In Acta histochemica, 125, 152072. doi:10.1016/j.acthis.2023.152072. https://pubmed.ncbi.nlm.nih.gov/37385108/
7. Gong, Binbin, Ma, Ming, Yang, Xiaorong, Luo, Yanping, Sun, Ting. 2019. MCM5 promotes tumour proliferation and correlates with the progression and prognosis of renal cell carcinoma. In International urology and nephrology, 51, 1517-1526. doi:10.1007/s11255-019-02169-3. https://pubmed.ncbi.nlm.nih.gov/31190295/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen