C57BL/6JCya-Meox2em1flox/Cya
Common Name:
Meox2-flox
Product ID:
S-CKO-03710
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Meox2-flox
Strain ID
CKOCMP-17286-Meox2-B6J-VA
Gene Name
Product ID
S-CKO-03710
Gene Alias
Gax; Mox-2; Mox2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
12
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Meox2em1flox/Cya mice (Catalog S-CKO-03710) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000041183
NCBI RefSeq
NM_008584
Target Region
Exon 2
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Meox2, also known as Mesenchyme Homeobox 2, is a transcription factor involved in mesoderm differentiation, including the development of bones, muscles, vasculature, and dermatomes [4]. It has been associated with multiple signaling pathways such as ERK, PI3K/AKT, and is of great biological importance in various physiological and pathological processes [1,5,6]. Genetic models like mouse models are valuable for studying its functions.
In gliomas, Meox2 acts as an oncogenic transcription regulator, promoting cell proliferation, motility, and tumorigenesis. Knockdown of Meox2 in patient-derived GBM tumorspheres inhibited growth and altered ERK phosphorylation [1]. In glioma cells, its inhibition changed cell morphology, hindered epithelial-mesenchymal transition, focal adhesion formation, and F-actin assembly, while overexpression had opposite effects [2].
In DBA/2J glaucoma mouse models, Meox2 haploinsufficiency accelerated axonal degeneration, indicating its role in controlling IOP-dependent vascular remodeling and neuroinflammation to promote axon survival [3].
In hepatic stellate cells, overexpression of Meox2 inhibited cell vitality and proliferation by regulating the PI3K/AKT signaling pathway, thus slowing liver fibrosis progression [5].
In glioma stem-like cells, loss-of-function of Meox2 affected cell viability, proliferation, and adhesion through AKT/ERK pathways [6]. In glioblastoma stem cells, Meox2 knockdown inhibited cell growth, sphere-forming ability, and increased apoptosis, and it was involved in regulating genes of the glycolytic pathway and response to hypoxia [7].
In conclusion, Meox2 has diverse functions in mesoderm-related development and multiple disease conditions. Mouse models, especially those with Meox2 haploinsufficiency or knockdown, have been crucial in revealing its role in gliomas, glaucoma, and liver fibrosis. These findings provide insights into potential therapeutic targets for these diseases.
References:
1. Schönrock, Anna, Heinzelmann, Elisa, Steffl, Bianca, Mall, Moritz, Turcan, Şevin. . MEOX2 homeobox gene promotes growth of malignant gliomas. In Neuro-oncology, 24, 1911-1924. doi:10.1093/neuonc/noac110. https://pubmed.ncbi.nlm.nih.gov/35468210/
2. Wang, Ji, Chen, Yanming, Wang, Qing, Zhang, Tan, Lan, Qing. 2022. MEOX2-mediated regulation of Cathepsin S promotes cell proliferation and motility in glioma. In Cell death & disease, 13, 360. doi:10.1038/s41419-022-04845-2. https://pubmed.ncbi.nlm.nih.gov/35436995/
3. Buchanan, Rebecca A, Foley, Kate E, Pepper, Keating W, John, Simon W M, Howell, Gareth R. . Meox2 Haploinsufficiency Accelerates Axonal Degeneration in DBA/2J Glaucoma. In Investigative ophthalmology & visual science, 60, 3283-3296. doi:10.1167/iovs.18-26126. https://pubmed.ncbi.nlm.nih.gov/31369031/
4. Kokotović, Tomislav, Lenartowicz, Ewelina M, Langeslag, Michiel, Penninger, Josef M, Nagy, Vanja. 2022. Transcription factor mesenchyme homeobox protein 2 (MEOX2) modulates nociceptor function. In The FEBS journal, 289, 3457-3476. doi:10.1111/febs.16347. https://pubmed.ncbi.nlm.nih.gov/35029322/
5. Wang, Qiong, Li, Ting, He, Yansha, Rao, Chunyan. . MEOX2 Participates in Hepatic Stellate Cells-Induced Liver Fibrosis by Regulating the PI3K/AKT Signaling Pathway. In Discovery medicine, 36, 1199-1209. doi:10.24976/Discov.Med.202436185.110. https://pubmed.ncbi.nlm.nih.gov/38926106/
6. Tachon, Gaëlle, Masliantsev, Konstantin, Rivet, Pierre, Karayan-Tapon, Lucie, Guichet, Pierre-Olivier. 2021. MEOX2 Transcription Factor Is Involved in Survival and Adhesion of Glioma Stem-like Cells. In Cancers, 13, . doi:10.3390/cancers13235943. https://pubmed.ncbi.nlm.nih.gov/34885053/
7. Proserpio, Carla, Galardi, Silvia, Desimio, Maria Giovanna, Matteucci, Claudia, Ciafrè, Silvia Anna. 2022. MEOX2 Regulates the Growth and Survival of Glioblastoma Stem Cells by Modulating Genes of the Glycolytic Pathway and Response to Hypoxia. In Cancers, 14, . doi:10.3390/cancers14092304. https://pubmed.ncbi.nlm.nih.gov/35565433/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen