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C57BL/6JCya-Metem1flox/Cya
Common Name:
Met-flox
Product ID:
S-CKO-03717
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Met-flox
Strain ID
CKOCMP-17295-Met-B6J-VA
Gene Name
Met
Product ID
S-CKO-03717
Gene Alias
HGF; HGFR; Par4; c-Met
Background
C57BL/6JCya
NCBI ID
17295
Modification
Conditional knockout
Chromosome
6
Phenotype
MGI:96969
Document
Click here to download >>
Application
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More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Metem1flox/Cya mice (Catalog S-CKO-03717) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000115443
NCBI RefSeq
NM_008591
Target Region
Exon 7
Size of Effective Region
~1.4 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Met, also known as the c-Met proto-oncogene, is a receptor tyrosine kinase. Its ligand is hepatocyte growth factor (HGF). The HGF/MET axis plays a key role in multiple biological processes such as cellular proliferation, migration, and invasion, which are crucial during embryonic development and tissue repair. Aberrant activation of this pathway due to mutations, fusions, amplification, or abnormal ligand production is associated with cancer development [1,2,3,4,5,6].

Developing MET-targeted therapies has been challenging. Historically, focusing on MET-expressing cancers had limited success as MET expression without a genomic marker of dependence poorly predicts treatment benefit [1]. However, high-level MET amplification, activating mutations (including kinase or extracellular domain mutations and exon 14 skipping), and fusions are now recognized as oncogenic drivers [1]. For instance, in non-small-cell lung cancer (NSCLC), MET exon 14 mutations are a distinct molecular subtype, often associated with advanced age, and stage-dependent MET genomic amplification and c-Met overexpression [7]. MET-targeted therapies' efficacy varies by MET alteration category, and higher levels of MET amplification increase the likelihood of benefit, though an optimal diagnostic cut-off for MET copy number gains is yet to be agreed upon [1]. In gliomas, the dysregulated MET signaling pathway is involved in tumor progression, and combined targeted therapy for this pathway could be a novel treatment strategy [5]. In renal cell carcinoma (RCC), the MET pathway is targeted by novel agents, and MET alterations can act as primary or secondary drivers of tumor growth [6].

In conclusion, Met is a crucial gene with its HGF/MET axis playing essential roles in normal biological processes and disease, especially cancer. Research on Met-targeted therapies has highlighted the significance of genomic alterations in predicting treatment response. Understanding Met's role through studies in various cancer types helps in developing more effective therapeutic strategies for cancer treatment.

References:
1. Guo, Robin, Luo, Jia, Chang, Jason, Arcila, Maria, Drilon, Alexander. 2020. MET-dependent solid tumours - molecular diagnosis and targeted therapy. In Nature reviews. Clinical oncology, 17, 569-587. doi:10.1038/s41571-020-0377-z. https://pubmed.ncbi.nlm.nih.gov/32514147/
2. Oliveres, Helena, Pineda, Estela, Maurel, Joan. 2019. MET inhibitors in cancer: pitfalls and challenges. In Expert opinion on investigational drugs, 29, 73-85. doi:10.1080/13543784.2020.1699532. https://pubmed.ncbi.nlm.nih.gov/31783719/
3. Kumaki, Yuichi, Oda, Goshi, Ikeda, Sadakatsu. 2023. Targeting MET Amplification: Opportunities and Obstacles in Therapeutic Approaches. In Cancers, 15, . doi:10.3390/cancers15184552. https://pubmed.ncbi.nlm.nih.gov/37760522/
4. Lombardi, Andrea Maria, Sangiolo, Dario, Vigna, Elisa. 2024. MET Oncogene Targeting for Cancer Immunotherapy. In International journal of molecular sciences, 25, . doi:10.3390/ijms25116109. https://pubmed.ncbi.nlm.nih.gov/38892318/
5. Cheng, Fangling, Guo, Dongsheng. 2019. MET in glioma: signaling pathways and targeted therapies. In Journal of experimental & clinical cancer research : CR, 38, 270. doi:10.1186/s13046-019-1269-x. https://pubmed.ncbi.nlm.nih.gov/31221203/
6. Nandagopal, Lakshminarayanan, Sonpavde, Guru P, Agarwal, Neeraj. 2019. Investigational MET inhibitors to treat Renal cell carcinoma. In Expert opinion on investigational drugs, 28, 851-860. doi:10.1080/13543784.2019.1673366. https://pubmed.ncbi.nlm.nih.gov/31554440/
7. Awad, Mark M, Oxnard, Geoffrey R, Jackman, David M, Hammerman, Peter S, Sholl, Lynette M. 2016. MET Exon 14 Mutations in Non-Small-Cell Lung Cancer Are Associated With Advanced Age and Stage-Dependent MET Genomic Amplification and c-Met Overexpression. In Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 34, 721-30. doi:10.1200/JCO.2015.63.4600. https://pubmed.ncbi.nlm.nih.gov/26729443/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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