C57BL/6JCya-Kitlem1flox/Cya
Common Name:
Kitl-flox
Product ID:
S-CKO-03726
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Kitl-flox
Strain ID
CKOCMP-17311-Kitl-B6J-VA
Gene Name
Product ID
S-CKO-03726
Gene Alias
Clo; Con; Gb; Kitlg; Mgf; SCF; SF; SLF; Sl; blz; contrasted
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Kitlem1flox/Cya mice (Catalog S-CKO-03726) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000105283
NCBI RefSeq
NM_013598
Target Region
Exon 5
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Kitl, also known as stem cell factor (SCF), is a paracrine signaling factor playing crucial roles in multiple biological processes. It is involved in maintaining tissue homeostasis, regulating hematopoietic stem cell function, and is essential for melanocyte development [1,2,3,5]. It participates in signaling pathways that impact cell proliferation, differentiation, and self-renewal [1,5]. Genetic models, such as gene-knockout (KO) and conditional-knockout (CKO) mouse models, are valuable tools for studying Kitl's functions.
In hematopoietic aging, reduced Kitl expression in mesenchymal stromal cells (MSCs) of middle-aged mice was highly correlated with reduced lymphoid lineage commitment of hematopoietic stem cells (HSCs) and increased signatures of differentiation-inactive HSCs [1]. In pancreas tissue, genetic inhibition of mesenchymal Kitl increased tumor growth and reduced survival of tumor-bearing mice, indicating that loss of stromal Kitl creates a tumor-permissive microenvironment [2,4]. In postnatal and adult mice, overexpression of Kitl in melanocytes influenced their proliferation, differentiation, and the self-renewal capacity of melanocyte stem cells, and also induced melanocyte migration from hair follicles to the epidermis [5]. Conditional deletion of Scf (Kitl) from endothelial cells or leptin receptor (Lepr)-expressing perivascular stromal cells depleted HSCs from the bone marrow [6].
In conclusion, Kitl is essential for maintaining tissue homeostasis, regulating hematopoietic stem cells, and influencing melanocyte development. Studies using KO/CKO mouse models have revealed its significant role in hematopoietic aging, pancreas tumor progression, and melanocyte-related processes. Understanding Kitl's functions provides insights into these biological processes and potential therapeutic targets for related diseases.
References:
1. Young, Kira A, Telpoukhovskaia, Maria A, Hofmann, Johanna, Kokkaliaris, Konstantinos D, Trowbridge, Jennifer J. . Variation in mesenchymal KITL/SCF and IGF1 expression in middle age underlies steady-state hematopoietic stem cell aging. In Blood, 144, 378-391. doi:10.1182/blood.2024024275. https://pubmed.ncbi.nlm.nih.gov/38598841/
2. Oñate, Maria Kathrina, Oon, Chet, Bhattacharyya, Sohinee, Xia, Zheng, Sherman, Mara H. . Stromal KITL/SCF Maintains Pancreas Tissue Homeostasis and Restrains Tumor Progression. In Cancer discovery, 15, 913-929. doi:10.1158/2159-8290.CD-24-1079. https://pubmed.ncbi.nlm.nih.gov/39918337/
3. França, Monica Malheiros, Mendonca, Berenice Bilharinho. 2021. Genetics of ovarian insufficiency and defects of folliculogenesis. In Best practice & research. Clinical endocrinology & metabolism, 36, 101594. doi:10.1016/j.beem.2021.101594. https://pubmed.ncbi.nlm.nih.gov/34794894/
4. Oñate, M Kathrina, Oon, Chet, Bhattacharyya, Sohinee, Xia, Zheng, Sherman, Mara H. 2024. Stromal KITL/SCF promotes pancreas tissue homeostasis and restrains tumor progression. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.07.29.605485. https://pubmed.ncbi.nlm.nih.gov/39131374/
5. Aoki, Hitomi, Tomita, Hiroyuki, Hara, Akira, Kunisada, Takahiro. 2023. Postnatal Expression of Kitl Affects Pigmentation of the Epidermis. In The Journal of investigative dermatology, 144, 96-105.e2. doi:10.1016/j.jid.2023.06.200. https://pubmed.ncbi.nlm.nih.gov/37482288/
6. Ding, Lei, Saunders, Thomas L, Enikolopov, Grigori, Morrison, Sean J. 2012. Endothelial and perivascular cells maintain haematopoietic stem cells. In Nature, 481, 457-62. doi:10.1038/nature10783. https://pubmed.ncbi.nlm.nih.gov/22281595/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen