C57BL/6JCya-Mitfem1flox/Cya
Common Name:
Mitf-flox
Product ID:
S-CKO-03736
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Mitf-flox
Strain ID
CKOCMP-17342-Mitf-B6J-VA
Gene Name
Product ID
S-CKO-03736
Gene Alias
BCC2; Bhlhe32; Gsfbcc2; Vitiligo; Wh; bw; mi; vit
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mitfem1flox/Cya mice (Catalog S-CKO-03736) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000043637
NCBI RefSeq
NM_001113198
Target Region
Exon 6
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Mitf, short for Microphthalmia-associated transcription factor, is a basic helix-loop-helix leucine zipper (bHLH-LZ) transcription factor. It is a key regulator in melanocyte development, melanogenesis, and melanocyte survival. MITF also participates in regulating cell proliferation, differentiation, and suppressing invasion, and is involved in pathways like MAPK and canonical Wnt/GSK3 [1,2,3]. Genetic models are valuable for studying its functions.
In zebrafish models, mitfa (the zebrafish ortholog of MITF) deficiency was found to accelerate GNAQ-driven uveal melanoma. The mitfa-deficient zebrafish showed a dramatic acceleration in the onset and progression of tumors induced by GNAQ or CYSLTR2 mutations, indicating that MITF acts as a tumor suppressor in uveal melanoma, contrary to its role in cutaneous melanoma [4].
In conclusion, MITF is essential for melanocyte-related biological processes. The zebrafish model with mitfa deficiency has revealed its significant role as a tumor suppressor in uveal melanoma, providing insights into the disease mechanism and potentially new strategies for uveal melanoma treatment.
References:
1. Gelmi, Maria Chiara, Houtzagers, Laurien E, Strub, Thomas, Krossa, Imène, Jager, Martine J. 2022. MITF in Normal Melanocytes, Cutaneous and Uveal Melanoma: A Delicate Balance. In International journal of molecular sciences, 23, . doi:10.3390/ijms23116001. https://pubmed.ncbi.nlm.nih.gov/35682684/
2. Louphrasitthiphol, Pakavarin, Loffreda, Alessia, Pogenberg, Vivian, Mazza, Davide, Goding, Colin R. 2023. Acetylation reprograms MITF target selectivity and residence time. In Nature communications, 14, 6051. doi:10.1038/s41467-023-41793-7. https://pubmed.ncbi.nlm.nih.gov/37770430/
3. Abrahamian, Carla, Grimm, Christian. 2021. Endolysosomal Cation Channels and MITF in Melanocytes and Melanoma. In Biomolecules, 11, . doi:10.3390/biom11071021. https://pubmed.ncbi.nlm.nih.gov/34356645/
4. Phelps, Grace B, Hagen, Hannah R, Amsterdam, Adam, Lees, Jacqueline A. 2022. MITF deficiency accelerates GNAQ-driven uveal melanoma. In Proceedings of the National Academy of Sciences of the United States of America, 119, e2107006119. doi:10.1073/pnas.2107006119. https://pubmed.ncbi.nlm.nih.gov/35512098/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen