C57BL/6JCya-Mitfem1flox/Cya
Common Name
Mitf-flox
Product ID
S-CKO-03736
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-17342-Mitf-B6J-VA
When using this mouse strain in a publication, please cite “Mitf-flox Mouse (Catalog S-CKO-03736) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Mitf-flox
Strain ID
CKOCMP-17342-Mitf-B6J-VA
Gene Name
Product ID
S-CKO-03736
Gene Alias
BCC2, Bhlhe32, Gsfbcc2, Vitiligo, Wh, bw, mi, vit
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 6
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000043637
NCBI RefSeq
NM_001113198
Target Region
Exon 6
Size of Effective Region
~1.2 kb
Overview of Gene Research
Mitf, short for Microphthalmia-associated transcription factor, is a basic helix-loop-helix leucine zipper (bHLH-LZ) transcription factor. It is a key regulator in melanocyte development, melanogenesis, and melanocyte survival. MITF also participates in regulating cell proliferation, differentiation, and suppressing invasion, and is involved in pathways like MAPK and canonical Wnt/GSK3 [1,2,3]. Genetic models are valuable for studying its functions.
In zebrafish models, mitfa (the zebrafish ortholog of MITF) deficiency was found to accelerate GNAQ-driven uveal melanoma. The mitfa-deficient zebrafish showed a dramatic acceleration in the onset and progression of tumors induced by GNAQ or CYSLTR2 mutations, indicating that MITF acts as a tumor suppressor in uveal melanoma, contrary to its role in cutaneous melanoma [4].
In conclusion, MITF is essential for melanocyte-related biological processes. The zebrafish model with mitfa deficiency has revealed its significant role as a tumor suppressor in uveal melanoma, providing insights into the disease mechanism and potentially new strategies for uveal melanoma treatment.
References：
1. Gelmi, Maria Chiara, Houtzagers, Laurien E, Strub, Thomas, Krossa, Imène, Jager, Martine J. 2022. MITF in Normal Melanocytes, Cutaneous and Uveal Melanoma: A Delicate Balance. In International journal of molecular sciences, 23, . doi:10.3390/ijms23116001. https://pubmed.ncbi.nlm.nih.gov/35682684/
2. Louphrasitthiphol, Pakavarin, Loffreda, Alessia, Pogenberg, Vivian, Mazza, Davide, Goding, Colin R. 2023. Acetylation reprograms MITF target selectivity and residence time. In Nature communications, 14, 6051. doi:10.1038/s41467-023-41793-7. https://pubmed.ncbi.nlm.nih.gov/37770430/
3. Abrahamian, Carla, Grimm, Christian. 2021. Endolysosomal Cation Channels and MITF in Melanocytes and Melanoma. In Biomolecules, 11, . doi:10.3390/biom11071021. https://pubmed.ncbi.nlm.nih.gov/34356645/
4. Phelps, Grace B, Hagen, Hannah R, Amsterdam, Adam, Lees, Jacqueline A. 2022. MITF deficiency accelerates GNAQ-driven uveal melanoma. In Proceedings of the National Academy of Sciences of the United States of America, 119, e2107006119. doi:10.1073/pnas.2107006119. https://pubmed.ncbi.nlm.nih.gov/35512098/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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