C57BL/6JCya-Aff1em1flox/Cya
Common Name
Aff1-flox
Product ID
S-CKO-03743
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-17355-Aff1-B6J-VA
When using this mouse strain in a publication, please cite “Aff1-flox Mouse (Catalog S-CKO-03743) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Aff1-flox
Strain ID
CKOCMP-17355-Aff1-B6J-VA
Gene Name
Product ID
S-CKO-03743
Gene Alias
Af4, Rob, Mllt2h, 9630032B01Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 5
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000054979
NCBI RefSeq
NM_133919
Target Region
Exon 2~3
Size of Effective Region
~1.9 kb
Overview of Gene Research
Aff1, also known as AF4/FMR2 family member 1, is a central scaffolding protein of the super elongation complex (SEC). It plays a crucial role in regulating gene transcription, which is essential for various biological processes [1,3,4,5,6]. The SEC, with Aff1 as a key component, is involved in controlling transcription elongation, and Aff1 has been associated with different cellular differentiation processes and disease conditions [1,3,4,5,6]. Genetic models, such as gene knockout (KO) and conditional knockout (CKO) mouse models, can be valuable for studying Aff1's functions.
In adipogenic differentiation, depletion of Aff1 in human mesenchymal stromal/stem cells (hMSCs) and 3T3-L1 pre-adipocytes enhanced adipogenesis, while overexpression of Aff1 in 3T3-L1 cells reduced adipogenic differentiation and adipose tissue formation in vivo. Mechanistically, Aff1 binds to the promoter region of the Tgm2 gene and regulates its transcription, and overexpression of Tgm2 rescues adipogenic differentiation of Aff1-deficient cells [1]. In osteogenic differentiation, siRNA-mediated depletion of Aff1 in human MSCs led to increased alkaline phosphatase (ALP) activity, enhanced mineralization, and up-regulated expression of osteogenic-related genes [4].
In conclusion, Aff1 plays an inhibitory role in adipogenic and osteogenic differentiations. These functions of Aff1, as revealed through model-based research, contribute to our understanding of cell differentiation processes. Additionally, in neonatal leukemia, the t(4;11)(q21.3;q23.3)/KMT2A-AFF1 rearrangement is the most frequently observed cytogenetic/molecular abnormality, highlighting the importance of Aff1-related research in understanding this disease [2].
References：
1. Chen, Yaqian, Wang, Yuan, Lin, Weimin, Zhou, Chenchen, Yuan, Quan. 2020. AFF1 inhibits adipogenic differentiation via targeting TGM2 transcription. In Cell proliferation, 53, e12831. doi:10.1111/cpr.12831. https://pubmed.ncbi.nlm.nih.gov/32441391/
2. Roberts, Irene, Fordham, Nicholas J, Rao, Anupama, Bain, Barbara J. 2018. Neonatal leukaemia. In British journal of haematology, 182, 170-184. doi:10.1111/bjh.15246. https://pubmed.ncbi.nlm.nih.gov/29806701/
3. Kumari, Nidhi, Hassan, M Abul, Lu, Xiangdong, Roeder, Robert G, Biswas, Debabrata. 2019. AFF1 acetylation by p300 temporally inhibits transcription during genotoxic stress response. In Proceedings of the National Academy of Sciences of the United States of America, 116, 22140-22151. doi:10.1073/pnas.1907097116. https://pubmed.ncbi.nlm.nih.gov/31611376/
4. Zhou, Chen-Chen, Xiong, Qiu-Chan, Zhu, Xin-Xing, Wang, Cun-Yu, Yuan, Quan. 2017. AFF1 and AFF4 differentially regulate the osteogenic differentiation of human MSCs. In Bone research, 5, 17044. doi:10.1038/boneres.2017.44. https://pubmed.ncbi.nlm.nih.gov/28955517/
5. Che, Zhuanzhuan, Liu, Xiaoxu, Dai, Qian, Luo, Zhuojuan, Lin, Chengqi. . Distinct roles of two SEC scaffold proteins, AFF1 and AFF4, in regulating RNA polymerase II transcription elongation. In Journal of molecular cell biology, 15, . doi:10.1093/jmcb/mjad049. https://pubmed.ncbi.nlm.nih.gov/37528066/
6. Lloyd, Sarah M, Leon, Daniel B, Brady, Mari O, Ho, Patric J, Bao, Xiaomin. 2022. CDK9 activity switch associated with AFF1 and HEXIM1 controls differentiation initiation from epidermal progenitors. In Nature communications, 13, 4408. doi:10.1038/s41467-022-32098-2. https://pubmed.ncbi.nlm.nih.gov/35906225/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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