C57BL/6NCya-Mstnem1flox/Cya
Common Name:
Mstn-flox
Product ID:
S-CKO-03802
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Mstn-flox
Strain ID
CKOCMP-17700-Mstn-B6N-VA
Gene Name
Product ID
S-CKO-03802
Gene Alias
Cmpt; Gdf8
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Mstnem1flox/Cya mice (Catalog S-CKO-03802) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027269
NCBI RefSeq
NM_010834
Target Region
Exon 2
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Myostatin (MSTN), also known as GDF-8, is a protein belonging to the transforming growth factor-β superfamily. It serves as a negative regulator of skeletal muscle growth and development, inhibiting the activation and proliferation of muscle satellite cells and myoblasts [1,3,4]. The MSTN regulatory network involves genes related to muscle function, cell-cycle regulation, and signaling pathways such as Notch, MAPK, and WNT [3]. Mutations in MSTN are of great commercial value as they can improve livestock meat production [2].
In various animal models, MSTN inactivation has shown significant effects. In MSTN-edited heterozygous pigs, there were no significant differences in the histomorphology of digestive and reproductive organs compared to wild-type pigs, though the MSTN +/- pigs had higher muscle depth and lower fat depth [1]. In sheep, a MSTNDel73C mutation with FGF5 knockout led to a dominant 'double-muscle' phenotype, increased muscle fiber number, and smaller myotubes through the activation of the MEK-ERK-FOSL1 axis [2]. In goats, MSTN gene mutations obtained by TALENs technology resulted in tightly arranged and thick muscle fibers and higher monthly body weight in cloned goats [4]. In cattle, MSTN knockout (- 12bps deletion) could be germline-transmitted to the next generation without health issues [5]. In Hu sheep, MSTN-edited animals using C-CRISPR showed a double-muscled phenotype with higher body weight at 3 and 4 months old [6].
In conclusion, MSTN is a key negative regulator of skeletal muscle growth. Studies using gene-edited animal models, such as KO models in pigs, sheep, goats, cattle, and Hu sheep, have revealed its role in muscle development and the potential for improving livestock production. These findings provide valuable insights into the biological function of MSTN and its potential applications in animal breeding and muscle-related disease treatment.
References:
1. Pei, Yangli, Fan, Ziyao, Song, Yuxin, Li, Hua, Li, Kui. 2022. Viscera Characteristics of MSTN-Edited Heterozygous Pigs. In Frontiers in genetics, 13, 764965. doi:10.3389/fgene.2022.764965. https://pubmed.ncbi.nlm.nih.gov/35299949/
2. Chen, Ming-Ming, Zhao, Yue, Yu, Kun, Lian, Di, Lian, Zheng-Xing. 2024. A MSTNDel73C mutation with FGF5 knockout sheep by Nuclease technology promotes skeletal muscle myofiber hyperplasia. In eLife, 12, . doi:10.7554/eLife.86827. https://pubmed.ncbi.nlm.nih.gov/39365728/
3. Budsuren, Undarmaa, Ulaangerel, Tseweendolmaa, Shen, Yingchao, Dugarjav, Manglai, Bou, Gerelchimeg. 2022. MSTN Regulatory Network in Mongolian Horse Muscle Satellite Cells Revealed with miRNA Interference Technologies. In Genes, 13, . doi:10.3390/genes13101836. https://pubmed.ncbi.nlm.nih.gov/36292721/
4. Song, Shao-Zheng, He, Zheng-Yi, Cheng, Yong, Zhang, Ting, Li, Dan. . MSTN modification in goat mediated by TALENs and performance analysis. In Yi chuan = Hereditas, 44, 531-542. doi:10.16288/j.yczz.22-011. https://pubmed.ncbi.nlm.nih.gov/35729101/
5. Gim, Gyeong-Min, Uhm, Kyeong-Hyun, Kwon, Dong-Hyeok, Lee, Won-Wu, Jang, Goo. 2022. Germline transmission of MSTN knockout cattle via CRISPR-Cas9. In Theriogenology, 192, 22-27. doi:10.1016/j.theriogenology.2022.08.021. https://pubmed.ncbi.nlm.nih.gov/36037573/
6. Guo, Rihong, Wang, Huili, Meng, Chunhua, Zhong, Jifeng, Cao, Shaoxian. 2023. Efficient and Specific Generation of MSTN-Edited Hu Sheep Using C-CRISPR. In Genes, 14, . doi:10.3390/genes14061216. https://pubmed.ncbi.nlm.nih.gov/37372396/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen