C57BL/6NCya-Maptem1flox/Cya
Common Name:
Mapt-flox
Product ID:
S-CKO-03818
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Mapt-flox
Strain ID
CKOCMP-17762-Mapt-B6N-VA
Gene Name
Product ID
S-CKO-03818
Gene Alias
Mtapt; PHF-tau; Tau
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Maptem1flox/Cya mice (Catalog S-CKO-03818) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000100347
NCBI RefSeq
NM_001038609
Target Region
Exon 7
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Mapt, short for microtubule-associated protein tau, encodes the tau protein which is crucial for maintaining microtubule function and axonal transport. Tau protein, through its association with microtubules, participates in various cellular processes related to neuronal structure and function [2,5]. The gene's expression and alternative splicing are complex, generating six main isoforms in the central nervous system, which play different roles in both physiological and pathological conditions [3,4].
In a study, through homologous recombination, the entire murine Mapt gene was replaced with the human ortholog, creating knock-in mice. These mice, with humanized Mapt, expressed all six human tau isoforms. When crossed with single amyloid precursor protein (App) knock-in mice, the double-knock-in mice showed higher tau phosphorylation. Also, tau humanization significantly accelerated cell-to-cell propagation of AD brain-derived pathological tau, both in the absence and presence of Aβ-amyloidosis. This demonstrated that Aβ-amyloidosis affects tau pathology and that pathological human tau interacts better with human tau than murine tau, suggesting species-specific differences [1].
In conclusion, Mapt plays a vital role in maintaining normal neuronal function through the tau protein it encodes. The development of Mapt humanized knock-in mouse models has provided valuable insights into the role of Mapt in neurodegenerative diseases, especially in understanding the relationship between tau pathology and Aβ-amyloidosis, which are key pathological features in diseases like Alzheimer's disease.
References:
1. Saito, Takashi, Mihira, Naomi, Matsuba, Yukio, Trojanowski, John Q, Saido, Takaomi C. 2019. Humanization of the entire murine Mapt gene provides a murine model of pathological human tau propagation. In The Journal of biological chemistry, 294, 12754-12765. doi:10.1074/jbc.RA119.009487. https://pubmed.ncbi.nlm.nih.gov/31273083/
2. Leveille, Etienne, Ross, Owen A, Gan-Or, Ziv. 2021. Tau and MAPT genetics in tauopathies and synucleinopathies. In Parkinsonism & related disorders, 90, 142-154. doi:10.1016/j.parkreldis.2021.09.008. https://pubmed.ncbi.nlm.nih.gov/34593302/
3. Corsi, Andrea, Bombieri, Cristina, Valenti, Maria Teresa, Romanelli, Maria Grazia. 2022. Tau Isoforms: Gaining Insight into MAPT Alternative Splicing. In International journal of molecular sciences, 23, . doi:10.3390/ijms232315383. https://pubmed.ncbi.nlm.nih.gov/36499709/
4. Ruiz-Gabarre, Daniel, Carnero-Espejo, Almudena, Ávila, Jesús, García-Escudero, Vega. 2022. What's in a Gene? The Outstanding Diversity of MAPT. In Cells, 11, . doi:10.3390/cells11050840. https://pubmed.ncbi.nlm.nih.gov/35269461/
5. Zhang, Cheng-Cheng, Xing, Ang, Tan, Meng-Shan, Tan, Lan, Yu, Jin-Tai. 2015. The Role of MAPT in Neurodegenerative Diseases: Genetics, Mechanisms and Therapy. In Molecular neurobiology, 53, 4893-904. doi:10.1007/s12035-015-9415-8. https://pubmed.ncbi.nlm.nih.gov/26363795/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen