C57BL/6JCya-Laptm4aem1flox/Cya
Common Name:
Laptm4a-flox
Product ID:
S-CKO-03827
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Laptm4a-flox
Strain ID
CKOCMP-17775-Laptm4a-B6J-VA
Gene Name
Product ID
S-CKO-03827
Gene Alias
LAPTM4; MTP; Mtrp; mKIAA0108
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
12
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Laptm4aem1flox/Cya mice (Catalog S-CKO-03827) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000020909
NCBI RefSeq
NM_008640
Target Region
Exon 2
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Laptm4a, also known as lysosomal-associated protein transmembrane 4A, is a protein-coding gene. It is involved in multiple cellular functions such as lysosomal membrane protein regulation, protein-protein interaction, and glycolipid regulation. It may participate in pathways related to endocytosis, intracellular sorting, and glycosphingolipid biosynthesis, which are crucial for maintaining normal cellular function and homeostasis [2,3,5,6,7].
In glioma, Laptm4a was found to be up-regulated and associated with poor prognosis. Functional enrichment analysis showed its role in the immune system and cancer progression. In vitro experiments indicated it may influence metastasis through the epithelial-mesenchymal transition (EMT) pathway. Also, patients with high Laptm4a expression were sensitive to doxorubicin [1].
In HeLa cells, disruption of Laptm4a gene reduced globotriaosylceramide (Gb3) biosynthesis, with loss of Laptm4a decreasing endogenous Gb3 synthase activity in a post-transcriptional mechanism [3].
In HK-2 cells, Laptm4a expression increased in high-glucose-induced cells, and the hsa_circ_0042260/miR-4782-3p/Lapmt4a axis was shown to play a role in regulating gestational diabetes mellitus (GDM) progression [4].
In human kidney, Laptm4a was demonstrated to interact with hOCT2, regulating its function by influencing its trafficking to/from the cell membrane [2].
In summary, Laptm4a plays essential roles in various biological processes, especially in the regulation of lysosomal function, glycolipid biosynthesis, and protein-protein interactions. Its dysregulation is associated with diseases like glioma and GDM. Studies on Laptm4a contribute to understanding the underlying mechanisms of these diseases, potentially providing new targets for diagnosis and treatment [1,3,4].
References:
1. Ding, Yongqi, Jiang, Yike, Zeng, Hong, Xiong, Chengfeng, Huang, Da. 2024. Identification of a robust biomarker LAPTM4A for glioma based on comprehensive computational biology and experimental verification. In Aging, 16, 6954-6989. doi:10.18632/aging.205736. https://pubmed.ncbi.nlm.nih.gov/38613802/
2. Grabner, A, Brast, S, Sucic, S, Schlatter, E, Ciarimboli, G. 2011. LAPTM4A interacts with hOCT2 and regulates its endocytotic recruitment. In Cellular and molecular life sciences : CMLS, 68, 4079-90. doi:10.1007/s00018-011-0694-6. https://pubmed.ncbi.nlm.nih.gov/21553234/
3. Yamaji, Toshiyuki, Sekizuka, Tsuyoshi, Tachida, Yuriko, Kuroda, Makoto, Hanada, Kentaro. 2019. A CRISPR Screen Identifies LAPTM4A and TM9SF Proteins as Glycolipid-Regulating Factors. In iScience, 11, 409-424. doi:10.1016/j.isci.2018.12.039. https://pubmed.ncbi.nlm.nih.gov/30660999/
4. Ji, Rui, Yang, Hong, Chen, Jiamei, Chen, Xia, Niu, Yanli. 2024. The role of hsa_circ_0042260/miR-4782-3p/LAPTM4A axis in gestational diabetes mellitus. In APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, 132, 465-476. doi:10.1111/apm.13407. https://pubmed.ncbi.nlm.nih.gov/38588560/
5. Zhang, Weichao, Yang, Xi, Chen, Liang, Wang, Yanzhuang, Li, Ming. 2021. A conserved ubiquitin- and ESCRT-dependent pathway internalizes human lysosomal membrane proteins for degradation. In PLoS biology, 19, e3001361. doi:10.1371/journal.pbio.3001361. https://pubmed.ncbi.nlm.nih.gov/34297722/
6. Milkereit, Ruth, Rotin, Daniela. 2011. A role for the ubiquitin ligase Nedd4 in membrane sorting of LAPTM4 proteins. In PloS one, 6, e27478. doi:10.1371/journal.pone.0027478. https://pubmed.ncbi.nlm.nih.gov/22096579/
7. Tian, Songhai, Muneeruddin, Khaja, Choi, Mei Yuk, Adam, Rosalyn M, Dong, Min. 2018. Genome-wide CRISPR screens for Shiga toxins and ricin reveal Golgi proteins critical for glycosylation. In PLoS biology, 16, e2006951. doi:10.1371/journal.pbio.2006951. https://pubmed.ncbi.nlm.nih.gov/30481169/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen