C57BL/6JCya-Mtx1em1flox/Cya
Common Name:
Mtx1-flox
Product ID:
S-CKO-03830
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Mtx1-flox
Strain ID
CKOCMP-17827-Mtx1-B6J-VA
Gene Name
Product ID
S-CKO-03830
Gene Alias
Gcap6; Mtx
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mtx1em1flox/Cya mice (Catalog S-CKO-03830) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000119222
NCBI RefSeq
NM_001357367
Target Region
Exon 3~5
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
MTX1, or Metaxin 1, is involved in multiple biological processes. In the context of hepatocellular carcinoma (HCC), it has been identified as a new regulator related to sorafenib resistance. MTX1 promotes HCC cell proliferation both in vitro and in vivo, increases cell growth rate, and decreases apoptosis upon sorafenib treatment. Mechanistically, it promotes autophagy in HCC cells by interacting with and inhibiting CDGSH iron sulfur domain 1 (CISD1), an autophagy negative regulator [1].
In the study of gout, MTX1 was identified among four genes related by protein-protein interaction network (PPI) analysis, suggesting its potential involvement in the pathogenesis of gout [2]. In Parkinson's disease (PD), homozygosity for the MTX1 c.184T>A (p.S63T) alteration was found to modify the age of onset in GBA-associated PD, with the homozygous MTX1 c.184A/A genotype associated with an earlier age of motor symptoms onset in patients with GBA mutations [3].
In conclusion, MTX1 plays important roles in multiple disease conditions such as HCC, gout, and PD. Its functions in promoting cell proliferation, autophagy, and its association with disease-related genetic alterations highlight its significance in understanding disease mechanisms. The studies on MTX1 contribute to the exploration of potential therapeutic targets and a better understanding of the underlying pathological processes in these diseases.
References:
1. Li, Li, Yu, Shijun, Hu, Qingqing, Hai, Yanan, Li, Yandong. 2021. Genome-scale CRISPRa screening identifies MTX1 as a contributor for sorafenib resistance in hepatocellular carcinoma by augmenting autophagy. In International journal of biological sciences, 17, 3133-3144. doi:10.7150/ijbs.62393. https://pubmed.ncbi.nlm.nih.gov/34421355/
2. Yang, Yubiao, Hu, Ping, Zhang, Qinnan, Hao, Jian, Zhou, Xianhu. 2024. Single-cell and genome-wide Mendelian randomization identifies causative genes for gout. In Arthritis research & therapy, 26, 114. doi:10.1186/s13075-024-03348-z. https://pubmed.ncbi.nlm.nih.gov/38831441/
3. Gan-Or, Ziv, Bar-Shira, Anat, Gurevich, Tanya, Giladi, Nir, Orr-Urtreger, Avi. 2011. Homozygosity for the MTX1 c.184T>A (p.S63T) alteration modifies the age of onset in GBA-associated Parkinson's disease. In Neurogenetics, 12, 325-32. doi:10.1007/s10048-011-0293-6. https://pubmed.ncbi.nlm.nih.gov/21837367/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen