C57BL/6JCya-Mx1em1flox/Cya
Common Name:
Mx1-flox
Product ID:
S-CKO-03840
Background:
C57BL/6JCya
Product Type
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Genotype
Sex
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Basic Information
Strain Name
Mx1-flox
Strain ID
CKOCMP-17857-Mx1-B6J-VA
Gene Name
Product ID
S-CKO-03840
Gene Alias
Mx; Mx-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
16
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mx1em1flox/Cya mice (Catalog S-CKO-03840) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000113768
NCBI RefSeq
NR_003520
Target Region
Exon 3~6
Size of Effective Region
~2.9 kb
Detailed Document
Overview of Gene Research
Mx1, also known as myxovirus resistance 1, encodes a GTPase that is an interferon-induced protein. It plays a crucial role in the innate antiviral immune response, restricting the replication of a variety of viruses, especially RNA viruses such as influenza A virus, Thogoto virus, and zoonotic avian influenza A virus [2,3,1]. Mx1 is considered an important part of the host's defense mechanism against viral infections, and its activity is independent of other interferon-induced genes in some cases [2].
In gene knockout studies, Mx1-/-mice were susceptible to influenza A virus challenge infection, indicating that Mx1 expression in the non-hematopoietic cellular compartment is required for protection against influenza virus [2]. Also, in the context of zoonotic H7N9 influenza virus, rare, heterozygous single-nucleotide variants in the MX1 gene were associated with increased human susceptibility, as most of the MxA variants identified lost the ability to inhibit H7N9, and nearly all of the inactive MxA variants exerted a dominant-negative effect on the antiviral function of wild-type MxA [1].
In conclusion, Mx1 is a key component of the innate antiviral immune response, protecting against various viral infections. Gene knockout mouse models have been instrumental in revealing its role in influenza virus and zoonotic H7N9 influenza virus infections, highlighting its importance in understanding the host-pathogen interaction and potential for developing antiviral strategies.
References:
1. Chen, Yongkun, Graf, Laura, Chen, Tao, Schwemmle, Martin, Shu, Yuelong. . Rare variant MX1 alleles increase human susceptibility to zoonotic H7N9 influenza virus. In Science (New York, N.Y.), 373, 918-922. doi:10.1126/science.abg5953. https://pubmed.ncbi.nlm.nih.gov/34413236/
2. Spitaels, Jan, Van Hoecke, Lien, Roose, Kenny, Kochs, Georg, Saelens, Xavier. 2019. Mx1 in Hematopoietic Cells Protects against Thogoto Virus Infection. In Journal of virology, 93, . doi:10.1128/JVI.00193-19. https://pubmed.ncbi.nlm.nih.gov/31092574/
3. Wagner, Valentina, Sabachvili, Mahana, Bendl, Elias, Fuchs, Jonas, Kochs, Georg. 2023. The Antiviral Activity of Equine Mx1 against Thogoto Virus Is Determined by the Molecular Structure of Its Viral Specificity Region. In Journal of virology, 97, e0193822. doi:10.1128/jvi.01938-22. https://pubmed.ncbi.nlm.nih.gov/36749070/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen