C57BL/6JCya-Myo5aem1flox/Cya
Common Name:
Myo5a-flox
Product ID:
S-CKO-03871
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Myo5a-flox
Strain ID
CKOCMP-17918-Myo5a-B6J-VA
Gene Name
Product ID
S-CKO-03871
Gene Alias
9630007J19Rik; Dbv; MVa; Myo5; MyoVA; Sev-1; d; d-120J; flail; flr
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Myo5aem1flox/Cya mice (Catalog S-CKO-03871) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000123128
NCBI RefSeq
NM_010864
Target Region
Exon 3
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Myo5a, coding for myosin VA, is involved in various cellular processes. It is crucial for melanosome transport, which is essential for pigmentation in organisms [2,3]. Myo5a is also likely to be associated with other biological functions related to cell migration and immune-related processes, as indicated by its roles in different diseases.
In head and neck squamous carcinoma (HNSC), Myo5a overexpression promotes cell migration in vitro, and its knockdown suppresses vimentin expression. It is a risk factor for human papillomavirus-positive (HPV+) HNSC, and high Myo5a expression in HNSC is correlated with low tumor-infiltrating lymphocytes, including activated CD4+ T cells, CD8+ T cells, and B cells [1]. In laryngeal squamous cell carcinoma, overexpression of serum Myo5a predicts cervical nodal occult metastasis and poor prognosis [4]. In esophageal squamous cell carcinoma, circFNDC3B promotes cancer progression by targeting Myo5a via miR-370-3p/miR-136-5p [5].
In conclusion, Myo5a plays important roles in pigmentation-related processes and is also significantly involved in the progression of multiple types of carcinomas. The study of Myo5a in these disease models helps to understand the underlying molecular mechanisms of tumorigenesis and metastasis, potentially providing new targets for cancer treatment.
References：
1. Xing, Juanli, Gu, Yanan, Song, Yichen, Ma, Junchi, Sui, Fang. 2023. MYO5A overexpression promotes invasion and correlates with low lymphocyte infiltration in head and neck squamous carcinoma. In BMC cancer, 23, 1267. doi:10.1186/s12885-023-11759-5. https://pubmed.ncbi.nlm.nih.gov/38129784/
2. Zhang, Hui, Wu, Zhongping, Yang, Lijuan, Chen, Hao, Ren, Jun. . Novel mutations in the Myo5a gene cause a dilute coat color phenotype in mice. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35, e21261. doi:10.1096/fj.201903141RR. https://pubmed.ncbi.nlm.nih.gov/33715225/
3. Christen, Matthias, de le Roi, Madeleine, Jagannathan, Vidhya, Becker, Kathrin, Leeb, Tosso. 2021. MYO5A Frameshift Variant in a Miniature Dachshund with Coat Color Dilution and Neurological Defects Resembling Human Griscelli Syndrome Type 1. In Genes, 12, . doi:10.3390/genes12101479. https://pubmed.ncbi.nlm.nih.gov/34680875/
4. Zhao, Xudong, Zhang, Wei, Ji, Wenyue. 2018. MYO5A inhibition by miR-145 acts as a predictive marker of occult neck lymph node metastasis in human laryngeal squamous cell carcinoma. In OncoTargets and therapy, 11, 3619-3635. doi:10.2147/OTT.S164597. https://pubmed.ncbi.nlm.nih.gov/29950866/
5. Song, Dan, Ye, Ziqi, Chen, Fangyu, Zhan, Liangliang, Sun, Xinchen. 2023. circFNDC3B promotes esophageal squamous cell carcinoma progression by targeting MYO5A via miR-370-3p/miR-136-5p. In BMC cancer, 23, 821. doi:10.1186/s12885-023-11314-2. https://pubmed.ncbi.nlm.nih.gov/37667251/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen