C57BL/6JCya-Ndufa4em1flox/Cya
Common Name:
Ndufa4-flox
Product ID:
S-CKO-03912
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Ndufa4-flox
Strain ID
CKOCMP-17992-Ndufa4-B6J-VA
Gene Name
Product ID
S-CKO-03912
Gene Alias
MLRQ
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ndufa4em1flox/Cya mice (Catalog S-CKO-03912) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000204978
NCBI RefSeq
NM_010886
Target Region
Exon 2
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
NDUFA4, also known as COXFA4, is a component of mitochondrial respiratory chain complex IV, playing a crucial role in mitochondrial energy metabolism [4]. It is involved in oxidative phosphorylation pathway, an essential process for proper cell functioning [2].
Ndufa4-knockout (KO) mice showed abnormal histological alterations in the brain tissue, along with impaired spatial learning capacity and exploratory activity. Ndufa4 depletion in neurons also led to changes in microRNA expressional profiles, where it inhibited miR-145a-5p expression, which in turn affected the proliferation and apoptosis of neurons [1].
In head and neck paraganglioma (HNPGL), NDUFA4 knockdown impaired the assembly of mitochondrial respiratory chain complexes, decreased ATP production and cell viability, and increased cell ferroptosis, suggesting its role in promoting HNPGL progression [2].
In pancreatic adenocarcinoma (PAAD), NDUFA4 knockdown reduced cell proliferation by inducing G1 arrest and suppressing oxidative phosphorylation, with high NDUFA4 levels associated with a poor survival rate [3].
In conclusion, Ndufa4 is essential for mitochondrial function and energy metabolism. Through gene-knockout mouse models, its role in various disease conditions such as neuronal disorders, HNPGL, and PAAD has been revealed. These studies help in understanding the biological functions of Ndufa4 and provide potential therapeutic targets for related diseases.
References：
1. Fu, Fang, Chen, Chen, Du, Kun, Li, Dong-Zhi, Liao, Can. 2023. Ndufa4 Regulates the Proliferation and Apoptosis of Neurons via miR-145a-5p/Homer1/Ccnd2. In Molecular neurobiology, 60, 2986-3003. doi:10.1007/s12035-023-03239-5. https://pubmed.ncbi.nlm.nih.gov/36763283/
2. Wang, Zhigang, Tao, Erxing, Chen, Yiming, Chen, Wei, Zhong, Chunlong. 2023. NDUFA4 promotes the progression of head and neck paraganglioma by inhibiting ferroptosis. In Biochemistry and cell biology = Biochimie et biologie cellulaire, 101, 523-530. doi:10.1139/bcb-2023-0018. https://pubmed.ncbi.nlm.nih.gov/37602474/
3. Zhang, Yue, Ge, Mengchen, Chen, Yuxiang, Chen, Xuemin, Wu, Xinquan. 2022. NDUFA4 promotes cell proliferation by enhancing oxidative phosphorylation in pancreatic adenocarcinoma. In Journal of bioenergetics and biomembranes, 54, 283-291. doi:10.1007/s10863-022-09949-0. https://pubmed.ncbi.nlm.nih.gov/36307669/
4. Pitceathly, Robert D S, Taanman, Jan-Willem. 2018. NDUFA4 (Renamed COXFA4) Is a Cytochrome-c Oxidase Subunit. In Trends in endocrinology and metabolism: TEM, 29, 452-454. doi:10.1016/j.tem.2018.03.009. https://pubmed.ncbi.nlm.nih.gov/29636225/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen