C57BL/6JCya-Neu1em1flox/Cya
Common Name:
Neu1-flox
Product ID:
S-CKO-03924
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Neu1-flox
Strain ID
CKOCMP-18010-Neu1-B6J-VA
Gene Name
Product ID
S-CKO-03924
Gene Alias
Aglp; Apl; Bat-7; Bat7; G9; Map-2; Neu; Neu-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Neu1em1flox/Cya mice (Catalog S-CKO-03924) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000007253
NCBI RefSeq
NM_010893
Target Region
Exon 3
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Neu1, also known as Neuraminidase 1, is a lysosomal sialidase that cleaves terminal α-linked sialic acid residues from sialylglycans [2]. It is ubiquitously expressed across various tissues and is crucial for lysosomal catabolic functions. Neu1 is involved in multiple pathways such as immune response modulation, elastic fiber assembly modulation, insulin signaling, and cell proliferation [1].
In a GS model mouse with homozygotic Ctsa IVS6 + 1 g/a mutation causing simultaneous deficiency of Ctsa and Neu1, symptoms like myoclonic seizures, suppressed behavior, and sialylglycan accumulation associated with neurovisceral inflammation were observed, indicating Neu1's role in preventing such pathological manifestations [2]. In Neu1-knockout zebrafish, abnormal emotional behavior was detected, along with changes in anxiety-related genes and lysosomal exocytosis-related genes, suggesting Neu1's role in normal emotional behavior [4]. In male mice, tubular epithelial cell-specific NEU1 knockout inhibited epithelial-to-mesenchymal transition, inflammatory cytokines production, and collagen deposition in renal fibrosis models, showing NEU1's promoting role in renal fibrosis [3].
In conclusion, Neu1 is essential for normal physiological functions, with its deficiency leading to various pathological conditions. Gene-knockout models in mice and zebrafish have been valuable in revealing its role in diseases such as sialidosis, neurodegeneration-related abnormal behaviors, and renal fibrosis, providing insights into potential therapeutic strategies targeting Neu1 for these diseases.
References:
1. Khan, Aiza, Sergi, Consolato M. 2022. NEU1-A Unique Therapeutic Target for Alzheimer's Disease. In Frontiers in pharmacology, 13, 902259. doi:10.3389/fphar.2022.902259. https://pubmed.ncbi.nlm.nih.gov/35847014/
2. Itoh, Kohji, Tsukimoto, Jun. 2023. Lysosomal sialidase NEU1, its intracellular properties, deficiency, and use as a therapeutic agent. In Glycoconjugate journal, 40, 611-619. doi:10.1007/s10719-023-10135-6. https://pubmed.ncbi.nlm.nih.gov/38147151/
3. Chen, Qian-Qian, Liu, Kang, Shi, Ning, Qi, Lian-Wen, Zhang, Lei. 2023. Neuraminidase 1 promotes renal fibrosis development in male mice. In Nature communications, 14, 1713. doi:10.1038/s41467-023-37450-8. https://pubmed.ncbi.nlm.nih.gov/36973294/
4. Ikeda, Asami, Komamizu, Mayu, Hayashi, Akito, Komatsu, Masaharu, Shiozaki, Kazuhiro. 2021. Neu1 deficiency induces abnormal emotional behavior in zebrafish. In Scientific reports, 11, 13477. doi:10.1038/s41598-021-92778-9. https://pubmed.ncbi.nlm.nih.gov/34188220/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen