C57BL/6JCya-Mycnem1flox/Cya
Common Name:
Mycn-flox
Product ID:
S-CKO-03980
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Mycn-flox
Strain ID
CKOCMP-18109-Mycn-B6J-VA
Gene Name
Product ID
S-CKO-03980
Gene Alias
N-myc; Nmyc; Nmyc-1; Nmyc1; bHLHe37; c-nmyc
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
12
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mycnem1flox/Cya mice (Catalog S-CKO-03980) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000043396
NCBI RefSeq
NM_008709
Target Region
Exon 2
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
MYCN, a member of the MYC family of oncogenes, encodes a nuclear protein functioning as a transcription factor. It binds active promoters in a heterodimer with MAX and is involved in RNA metabolism as it interacts with the nuclear exosome [1]. MYCN controls multiple aspects of cell fate such as cellular proliferation, differentiation, apoptosis, and cellular metabolism [5]. It has a restricted spatiotemporal expression predominantly in neural crest cells, which is associated with neoplasms like neuroblastoma and central nervous system tumours [5].
In neuroblastoma, MYCN amplification, seen in about 25% of cases, is strongly correlated with high-risk disease and poor prognosis [3,4,5]. In MYCN-amplified neuroblastoma cells, targeting the 5' untranslated region (UTR) of MYCN using an amidino-rocaglate to inhibit eIF4A1 activity led to growth inhibition without generalized toxicity, highlighting the therapeutic potential of this approach [2]. Also, MYCN drives oncogenesis in neuroblastoma by cooperating with the histone methyltransferase G9a and the WDR5 adaptor to regulate global gene transcription. Targeting both WDR5 and G9a inhibitors synergistically suppresses NB growth [6].
In conclusion, MYCN is a crucial regulator in cell fate-related processes. Its amplification in neuroblastoma is a significant prognostic factor. Studies in neuroblastoma models have provided insights into potential therapeutic strategies targeting MYCN-associated pathways, offering hope for improving patient outcomes in this high-risk disease.
References:
1. Papadopoulos, Dimitrios, Ha, Stefanie Anh, Fleischhauer, Daniel, Vos, Seychelle M, Eilers, Martin. 2024. The MYCN oncoprotein is an RNA-binding accessory factor of the nuclear exosome targeting complex. In Molecular cell, 84, 2070-2086.e20. doi:10.1016/j.molcel.2024.04.007. https://pubmed.ncbi.nlm.nih.gov/38703770/
2. Volegova, Marina P, Brown, Lauren E, Banerjee, Ushashi, Porco, John A, George, Rani E. 2024. The MYCN 5' UTR as a therapeutic target in neuroblastoma. In Cell reports, 43, 114134. doi:10.1016/j.celrep.2024.114134. https://pubmed.ncbi.nlm.nih.gov/38662542/
3. Huang, Miller, Weiss, William A. 2013. Neuroblastoma and MYCN. In Cold Spring Harbor perspectives in medicine, 3, a014415. doi:10.1101/cshperspect.a014415. https://pubmed.ncbi.nlm.nih.gov/24086065/
4. Schwab, Manfred. . MYCN in neuronal tumours. In Cancer letters, 204, 179-87. doi:. https://pubmed.ncbi.nlm.nih.gov/15013217/
5. Bhardwaj, Neha, Das, Gargi, Srinivasan, Radhika. 2023. Neuroblastoma-derived v-myc avian myelocytomatosis viral related oncogene or MYCN gene. In Journal of clinical pathology, 76, 518-523. doi:10.1136/jcp-2022-208476. https://pubmed.ncbi.nlm.nih.gov/37221048/
6. Liu, Zhihui, Zhang, Xiyuan, Xu, Man, Shern, Jack F, Thiele, Carol J. 2024. MYCN drives oncogenesis by cooperating with the histone methyltransferase G9a and the WDR5 adaptor to orchestrate global gene transcription. In PLoS biology, 22, e3002240. doi:10.1371/journal.pbio.3002240. https://pubmed.ncbi.nlm.nih.gov/38547242/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen