C57BL/6JCya-Emc8em1flox/Cya
Common Name:
Emc8-flox
Product ID:
S-CKO-03985
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Emc8-flox
Strain ID
CKOCMP-18117-Emc8-B6J-VA
Gene Name
Product ID
S-CKO-03985
Gene Alias
Cox4nb; Fam158b; Noc4
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Emc8em1flox/Cya mice (Catalog S-CKO-03985) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000034277
NCBI RefSeq
NM_010926
Target Region
Exon 2
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Emc8, a component of the endoplasmic reticulum membrane protein complex (EMC), is crucial for the insertion of diverse transmembrane proteins into the plasma membrane. Each EMC consists of Emc1-7, Emc10, and either Emc8 or Emc9. EMC plays a key role in the biogenesis of multi-pass transmembrane proteins, with implications for various biological processes [2,3].
In a study on systemic lupus erythematosus (SLE), it was found that fecal microbiota transplantation (FMT) led to an increase in the methylation level of the Emc8 promoter region in responders, suggesting a potential link between Emc8 methylation and the therapeutic mechanism of FMT in SLE [1]. In Drosophila, the loss of EMC components including Emc8/9 affects the biosynthesis of rhodopsin and other multi-pass membrane proteins, leading to retinal degeneration, highlighting its importance in maintaining the stability of multi-pass transmembrane proteins [3].
In conclusion, Emc8 is essential for the biogenesis of multi-pass transmembrane proteins. Studies in Drosophila and on SLE patients' methylation changes after FMT have revealed its significance in maintaining normal biological functions and its potential involvement in autoimmune diseases, providing insights into the underlying mechanisms of these biological processes and diseases.
References：
1. Zhang, Bo, Zhou, Wenhui, Liu, Qianmei, Zhao, Ming, Lu, Qianjin. 2023. Effects of fecal microbiota transplant on DNA methylation in patients with systemic lupus erythematosus. In Journal of autoimmunity, 141, 103047. doi:10.1016/j.jaut.2023.103047. https://pubmed.ncbi.nlm.nih.gov/37179169/
2. Marquez, Jonathan, Aslam, Faiza, Khokha, Mustafa K. 2023. Expanding EMC foldopathies: Topogenesis deficits alter the neural crest. In Genesis (New York, N.Y. : 2000), 61, e23520. doi:10.1002/dvg.23520. https://pubmed.ncbi.nlm.nih.gov/37318954/
3. Satoh, Takunori, Ohba, Aya, Liu, Ziguang, Inagaki, Tsuyoshi, Satoh, Akiko K. 2015. dPob/EMC is essential for biosynthesis of rhodopsin and other multi-pass membrane proteins in Drosophila photoreceptors. In eLife, 4, . doi:10.7554/eLife.06306. https://pubmed.ncbi.nlm.nih.gov/25715730/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen