C57BL/6JCya-Nr1i2em1flox/Cya
Common Name
Nr1i2-flox
Product ID
S-CKO-04018
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-18171-Nr1i2-B6J-VA
When using this mouse strain in a publication, please cite “Nr1i2-flox Mouse (Catalog S-CKO-04018) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Nr1i2-flox
Strain ID
CKOCMP-18171-Nr1i2-B6J-VA
Gene Name
Product ID
S-CKO-04018
Gene Alias
PXR, SXR, PXR1, mPXR, PXR.1, PXR.2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 16
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000023504
NCBI RefSeq
NM_010936
Target Region
Exon 3~4
Size of Effective Region
~1.1 kb
Overview of Gene Research
Nr1i2, also known as the pregnane X receptor (PXR), is a nuclear receptor classified as a xenoreceptor. It is activated by various xenobiotics, including drugs, and regulates the transcription level of major drug-metabolizing enzymes and transporters, facilitating xenobiotic elimination from the body [1]. It also has a role in the regulation of intestinal inflammation and fibrosis, and is involved in maintaining mucosal integrity [2,3].
In fibrotic complications of inflammatory bowel diseases (IBDs), Nr1i2-deficient mice showed exacerbated fibrosis after DSS-induced colitis, with greater neutrophil infiltration and innate cytokine production compared to wild-type mice. Deletion of PXR in fibroblasts, but not the epithelium, recapitulated this phenotype [2]. In addition, Nr1i2-deficient myofibroblasts were hyperresponsive to stimulation, producing increased levels of inflammatory mediators [2].
In conclusion, Nr1i2 plays a crucial role in drug metabolism and in regulating intestinal inflammation and fibrosis. Studies using Nr1i2-deficient mouse models have revealed its significance in the development of fibrotic complications in IBDs, highlighting its potential as a therapeutic target in such disease areas.
References：
1. Mbatchi, Litaty Céphanoée, Brouillet, Jean-Paul, Evrard, Alexandre. 2017. Genetic variations of the xenoreceptors NR1I2 and NR1I3 and their effect on drug disposition and response variability. In Pharmacogenomics, 19, 61-77. doi:10.2217/pgs-2017-0121. https://pubmed.ncbi.nlm.nih.gov/29199543/
2. Flannigan, Kyle L, Nieves, Kristoff M, Szczepanski, Holly E, Mani, Sridhar, Hirota, Simon A. 2022. The Pregnane X Receptor and Indole-3-Propionic Acid Shape the Intestinal Mesenchyme to Restrain Inflammation and Fibrosis. In Cellular and molecular gastroenterology and hepatology, 15, 765-795. doi:10.1016/j.jcmgh.2022.10.014. https://pubmed.ncbi.nlm.nih.gov/36309199/
3. Venkatesh, Madhukumar, Mukherjee, Subhajit, Wang, Hongwei, Khanna, Kamal M, Mani, Sridhar. 2014. Symbiotic bacterial metabolites regulate gastrointestinal barrier function via the xenobiotic sensor PXR and Toll-like receptor 4. In Immunity, 41, 296-310. doi:10.1016/j.immuni.2014.06.014. https://pubmed.ncbi.nlm.nih.gov/25065623/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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