C57BL/6JCya-Nrp1em1flox/Cya
Common Name:
Nrp1-flox
Product ID:
S-CKO-04027
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Nrp1-flox
Strain ID
CKOCMP-18186-Nrp1-B6J-VA
Gene Name
Product ID
S-CKO-04027
Gene Alias
C530029I03; NP-1; NPN-1; Npn1; Nrp
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nrp1em1flox/Cya mice (Catalog S-CKO-04027) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000026917
NCBI RefSeq
NM_008737
Target Region
Exon 2
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
Nrp1, or neuropilin 1, is expressed by neurons, blood vessels, immune cells, and many other cell types in the mammalian body. It binds a range of extracellular ligands to modulate organ development and function, playing roles in signaling pathways relevant to neuronal and vascular development in the brain and retina [2]. Mouse knockout models have been crucial tools for studying Nrp1 function [2].
Nrp1 has been found to be a receptor for mammalian orthoreovirus, contributing to viral dissemination and neurovirulence in mice. Capsid mutants unable to bind Nrp1 helped uncover this role [1]. In allergic airway inflammation, Nrp1 is a surface marker of ILC2s, with its down-regulation correlating with enhanced ILC2 responses during IL-33 challenge [3]. In liver fibrosis, Nrp1 expression is elevated in fibrotic livers, promoting HSC activation, and USP9X-mediated Nrp1 deubiquitination enhances this process [4]. In prostate cancer, Nrp1 promotes cancer progression by modulating the EGFR-dependent AKT pathway, and its inhibitor can suppress this process [5]. In colitis, Nrp1 is a positive regulator of IL-17-producing ILC3s, and its genetic deficiency or pharmacological inhibition can improve colitis outcomes [6].
In conclusion, Nrp1 is essential in various biological processes and disease conditions. Gene knockout models in mice have been instrumental in revealing its roles in viral pathogenesis, allergic inflammation, liver fibrosis, cancer progression, and colitis. Understanding Nrp1's functions through these models provides potential therapeutic targets for treating related diseases.
References:
1. Shang, Pengcheng, Dos Santos Natividade, Rita, Taylor, Gwen M, Alsteens, David, Dermody, Terence S. 2024. NRP1 is a receptor for mammalian orthoreovirus engaged by distinct capsid subunits. In Cell host & microbe, 32, 980-995.e9. doi:10.1016/j.chom.2024.04.014. https://pubmed.ncbi.nlm.nih.gov/38729153/
2. Raimondi, Claudio, Brash, James T, Fantin, Alessandro, Ruhrberg, Christiana. 2016. NRP1 function and targeting in neurovascular development and eye disease. In Progress in retinal and eye research, 52, 64-83. doi:10.1016/j.preteyeres.2016.02.003. https://pubmed.ncbi.nlm.nih.gov/26923176/
3. Wang, Ying, Liu, Gaoyu, Wang, Jianye, Liu, Qiang, Zhou, Jie. 2024. NRP1 downregulation correlates with enhanced ILC2 responses during IL-33 challenge. In Immunology, 172, 226-234. doi:10.1111/imm.13769. https://pubmed.ncbi.nlm.nih.gov/38409805/
4. Zhao, Jinqiu, Bai, Jie, Peng, Fengling, Li, Yongguo, Zhong, Li. 2023. USP9X-mediated NRP1 deubiquitination promotes liver fibrosis by activating hepatic stellate cells. In Cell death & disease, 14, 40. doi:10.1038/s41419-022-05527-9. https://pubmed.ncbi.nlm.nih.gov/36653359/
5. Zhang, Peng, Chen, Liang, Zhou, Fenfang, Wang, Gang, Luo, Yongwen. 2023. NRP1 promotes prostate cancer progression via modulating EGFR-dependent AKT pathway activation. In Cell death & disease, 14, 159. doi:10.1038/s41419-023-05696-1. https://pubmed.ncbi.nlm.nih.gov/36841806/
6. Wang, Ying, Wang, Jianye, Liu, Gaoyu, Wang, Haitao, Zhou, Jie. 2025. NRP1 instructs IL-17-producing ILC3s to drive colitis progression. In Cellular & molecular immunology, 22, 161-175. doi:10.1038/s41423-024-01246-7. https://pubmed.ncbi.nlm.nih.gov/39741194/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen