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C57BL/6JCya-Ntn1em1flox/Cya
Common Name:
Ntn1-flox
Product ID:
S-CKO-04042
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ntn1-flox
Strain ID
CKOCMP-18208-Ntn1-B6J-VA
Gene Name
Ntn1
Product ID
S-CKO-04042
Gene Alias
Netrin-1
Background
C57BL/6JCya
NCBI ID
18208
Modification
Conditional knockout
Chromosome
11
Phenotype
MGI:105088
Document
Click here to download >>
Application
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Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ntn1em1flox/Cya mice (Catalog S-CKO-04042) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000021284
NCBI RefSeq
NM_008744
Target Region
Exon 2
Size of Effective Region
~1.5 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Ntn1, also known as Netrin-1, is a gene that plays diverse and significant roles in various biological processes. It is involved in processes such as axon guidance, cell migration, and angiogenesis. In the context of signaling pathways, it can interact with multiple receptors like UNC5B to regulate different cellular responses, and is important for normal development and tissue homeostasis [4,5].

In porcine studies, NTN1-CNV affects intramuscular fat (IMF) content. Knock-down and over-expression experiments in C2C12 cells showed that NTN1 influences the proliferation and differentiation of muscle cells, potentially via the AMP-activated protein kinase (AMPK) pathway [1]. In cancer research, netrin-1 blockade using an anti-netrin-1 antibody (NP137) inhibits tumour growth and epithelial-to-mesenchymal transition (EMT) in endometrial cancer and skin squamous cell carcinoma (SCC) [2,3]. In mouse models of peripheral nerve injury, NTN1-high endothelial cells and their exosomes are critical for the formation of the vascular niche, promoting nerve repair [4]. In atherosclerotic mouse models, silencing myeloid Ntn1 reduces plaque burden and promotes inflammation resolution [6].

In conclusion, Ntn1 is essential for multiple biological processes including muscle development, tumour progression, nerve repair, and atherosclerosis. Gene-knockout or conditional-knockout mouse models, along with in vitro cell-based experiments, have been crucial in uncovering these functions. Understanding Ntn1's role provides potential therapeutic targets for various diseases such as cancer, nerve injury, and atherosclerosis.

References:
1. Wang, Ligang, Zhao, Lingling, Zhang, Longchao, Zhao, Fuping, Wang, Lixian. 2019. NTN1 Affects Porcine Intramuscular Fat Content by Affecting the Expression of Myogenic Regulatory Factors. In Animals : an open access journal from MDPI, 9, . doi:10.3390/ani9090609. https://pubmed.ncbi.nlm.nih.gov/31461826/
2. Cassier, Philippe A, Navaridas, Raul, Bellina, Melanie, Bernet, Agnès, Mehlen, Patrick. 2023. Netrin-1 blockade inhibits tumour growth and EMT features in endometrial cancer. In Nature, 620, 409-416. doi:10.1038/s41586-023-06367-z. https://pubmed.ncbi.nlm.nih.gov/37532934/
3. Lengrand, Justine, Pastushenko, Ievgenia, Vanuytven, Sebastiaan, Bernet, Agnès, Blanpain, Cédric. 2023. Pharmacological targeting of netrin-1 inhibits EMT in cancer. In Nature, 620, 402-408. doi:10.1038/s41586-023-06372-2. https://pubmed.ncbi.nlm.nih.gov/37532929/
4. Huang, Jinsheng, Li, Jiangnan, Li, Senrui, Wang, Yuanyi, Zhou, Nan. 2024. Netrin-1-engineered endothelial cell exosomes induce the formation of pre-regenerative niche to accelerate peripheral nerve repair. In Science advances, 10, eadm8454. doi:10.1126/sciadv.adm8454. https://pubmed.ncbi.nlm.nih.gov/38941462/
5. Zhang, Yuanda, Lan, Jin, Zhao, Dongxu, Tan, Haoyuan, Bao, Yinghui. 2022. Netrin-1 upregulates GPX4 and prevents ferroptosis after traumatic brain injury via the UNC5B/Nrf2 signaling pathway. In CNS neuroscience & therapeutics, 29, 216-227. doi:10.1111/cns.13997. https://pubmed.ncbi.nlm.nih.gov/36468399/
6. Schlegel, Martin, Sharma, Monika, Brown, Emily J, Fisher, Edward A, Moore, Kathryn J. 2021. Silencing Myeloid Netrin-1 Induces Inflammation Resolution and Plaque Regression. In Circulation research, 129, 530-546. doi:10.1161/CIRCRESAHA.121.319313. https://pubmed.ncbi.nlm.nih.gov/34289717/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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