C57BL/6JCya-Odc1em1flox/Cya
Common Name:
Odc1-flox
Product ID:
S-CKO-04066
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Odc1-flox
Strain ID
CKOCMP-18263-Odc1-B6J-VA
Gene Name
Product ID
S-CKO-04066
Gene Alias
ODC
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
12
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Odc1em1flox/Cya mice (Catalog S-CKO-04066) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000171737
NCBI RefSeq
NM_013614
Target Region
Exon 2~11
Size of Effective Region
~3.6 kb
Detailed Document
Overview of Gene Research
Odc1, or ornithine decarboxylase 1, is a key enzyme in polyamine biosynthesis, catalyzing the conversion of L-ornithine to polyamines. Polyamines are involved in multiple cellular processes such as cell differentiation, proliferation, and migration [3,4,5]. Odc1 has been linked to various biological pathways and is associated with many diseases, including cancer, neurodevelopmental disorders, and osteoporosis. Genetic models, like KO or CKO mouse models, can be valuable for studying its function.
Loss-of-function studies in some contexts have revealed important roles of Odc1. In osteoclasts, loss of Nrf2 upregulates Odc1, which decreases ornithine levels and promotes osteoclast differentiation, suggesting Odc1's role in bone-related processes [1]. In neuroblastoma, knockdown of Odc1 significantly attenuated the promotion effect of CDC27 on the proliferation, metastasis, and sphere-formation ability of NB cells, and also reversed the pro-ferroptotic effect of CDC27, indicating its role in tumorigenesis and ferroptosis regulation in this cancer type [2]. In hepatocellular carcinoma, decreasing ODC1 expression inhibits cell proliferation, migration, invasion, and induces cell cycle arrest both in vitro and in vivo, and ODC1 silencing also inhibits the growth of human HCC xenografts in nude mice, highlighting its role in cancer progression [4,5].
In conclusion, Odc1 is crucial for polyamine biosynthesis and involved in various biological processes. Model-based research, especially through KO/CKO mouse models, has shown its significance in diseases like osteoporosis, neuroblastoma, and hepatocellular carcinoma. Understanding Odc1's function provides insights into disease mechanisms and potential therapeutic targets for these conditions.
References:
1. Dong, Yimin, Kang, Honglei, Peng, Renpeng, Li, Feng, Song, Kehan. 2024. A clinical-stage Nrf2 activator suppresses osteoclast differentiation via the iron-ornithine axis. In Cell metabolism, 36, 1679-1695.e6. doi:10.1016/j.cmet.2024.03.005. https://pubmed.ncbi.nlm.nih.gov/38569557/
2. Qiu, Lin, Zhou, Rui, Luo, Ziyan, Wu, Jiangxue, Jiang, Hua. 2022. CDC27-ODC1 Axis Promotes Metastasis, Accelerates Ferroptosis and Predicts Poor Prognosis in Neuroblastoma. In Frontiers in oncology, 12, 774458. doi:10.3389/fonc.2022.774458. https://pubmed.ncbi.nlm.nih.gov/35242701/
3. Jiang, Fang, Gao, Yue, Dong, Chunsheng, Xiong, Sidong. 2018. ODC1 inhibits the inflammatory response and ROS-induced apoptosis in macrophages. In Biochemical and biophysical research communications, 504, 734-741. doi:10.1016/j.bbrc.2018.09.023. https://pubmed.ncbi.nlm.nih.gov/30217446/
4. Ye, Zi, Zeng, Zhirui, Shen, Yiyi, Chen, Zubing, Shen, Shiqiang. 2019. ODC1 promotes proliferation and mobility via the AKT/GSK3β/β-catenin pathway and modulation of acidotic microenvironment in human hepatocellular carcinoma. In OncoTargets and therapy, 12, 4081-4092. doi:10.2147/OTT.S198341. https://pubmed.ncbi.nlm.nih.gov/31239700/
5. Choi, Yunseon, Oh, Sang Taek, Won, Min-Ah, Seo, Jun-Young, Lee, Yun-Han. 2016. Targeting ODC1 inhibits tumor growth through reduction of lipid metabolism in human hepatocellular carcinoma. In Biochemical and biophysical research communications, 478, 1674-81. doi:10.1016/j.bbrc.2016.09.002. https://pubmed.ncbi.nlm.nih.gov/27592554/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen