C57BL/6JCya-Prdx1em1flox/Cya
Common Name:
Prdx1-flox
Product ID:
S-CKO-04157
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Prdx1-flox
Strain ID
CKOCMP-18477-Prdx1-B6J-VA
Gene Name
Product ID
S-CKO-04157
Gene Alias
MSP23; NkefA; OSF-3; OSF3; PAG; Paga; PrdxI; PrxI; TDX2; TPxA; Tdpx2; prx1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Prdx1em1flox/Cya mice (Catalog S-CKO-04157) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000135573
NCBI RefSeq
NM_011034
Target Region
Exon 3
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Prdx1, short for peroxiredoxin 1, is a member of the peroxidase family. It functions mainly in protecting cells against damage caused by H2O2 and also acts as a molecular chaperone in various biological processes. Prdx1 is involved in multiple pathways such as TLR4-NFκB signaling, autophagy, and redox-regulation, which are crucial for maintaining cellular homeostasis and are related to many disease-related processes [2].
In a study using PRDX1-knockdown (PRDX1KD) cells, it was found that PRDX1 negatively regulates TLR4 signaling for NFκB activation and autophagy functions. PRDX1KD THP-1 cells showed increased NFκB activation, pro-inflammatory cytokine production, and resistance against Salmonella typhimurium infection. Also, the migration and invasion abilities of PRDX1KD cancer cells were enhanced, indicating its role in cancer-related processes [1]. In PRDX1 knockout mice, the number of AOM/DSS-induced colitis-associated CRC was significantly lower, accompanied by downregulation of NRF2 and GPX4. Mechanistically, PRDX1 knockdown led to ROS-induced mitochondrial dysfunction and lipid peroxidation-induced ferroptosis in CRC cells [3].
In conclusion, Prdx1 plays essential roles in maintaining cellular redox balance, regulating autophagy, and influencing cancer-related processes. Gene knockout models, especially in mice, have provided significant insights into its functions in diseases like cancer and inflammatory bowel diseases, helping to understand the underlying mechanisms and potentially paving the way for new therapeutic strategies targeting Prdx1.
References:
1. Min, Yoon, Kim, Mi-Jeong, Lee, Sena, Chun, Eunyoung, Lee, Ki-Young. 2018. Inhibition of TRAF6 ubiquitin-ligase activity by PRDX1 leads to inhibition of NFKB activation and autophagy activation. In Autophagy, 14, 1347-1358. doi:10.1080/15548627.2018.1474995. https://pubmed.ncbi.nlm.nih.gov/29929436/
2. Guan, Xin, Ruan, Yiyin, Che, Xiaoxia, Feng, Weiwei. 2023. Dual role of PRDX1 in redox-regulation and tumorigenesis: Past and future. In Free radical biology & medicine, 210, 120-129. doi:10.1016/j.freeradbiomed.2023.11.009. https://pubmed.ncbi.nlm.nih.gov/37977211/
3. Song, Yujia, Wang, Xiaohui, Sun, Yuqi, Qu, Xianjun, Yu, Xinfeng. 2024. PRDX1 inhibits ferroptosis by binding to Cullin-3 as a molecular chaperone in colorectal cancer. In International journal of biological sciences, 20, 5070-5086. doi:10.7150/ijbs.99804. https://pubmed.ncbi.nlm.nih.gov/39430237/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen