C57BL/6JCya-Pck1em1flox/Cya
Common Name:
Pck1-flox
Product ID:
S-CKO-04181
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Pck1-flox
Strain ID
CKOCMP-18534-Pck1-B6J-VA
Gene Name
Product ID
S-CKO-04181
Gene Alias
PEPCK; PEPCK-C; Pck-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pck1em1flox/Cya mice (Catalog S-CKO-04181) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029017
NCBI RefSeq
NM_011044
Target Region
Exon 4~5
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Pck1, also known as phosphoenolpyruvate carboxykinase 1 (PEPCK1), is a key rate-limiting enzyme in gluconeogenesis, catalyzing the conversion of oxaloacetate to phosphoenolpyruvate [4]. It is involved in multiple biological processes and metabolic pathways, such as maintaining fasting glucose levels, affecting renal physiology, and being related to ammoniagenesis and cataplerosis in the liver [3,5]. Genetic models, especially KO and CKO mouse models, have been crucial in studying its functions.
In male mice, liver Pck1 deficiency promotes metabolic-associated fatty liver disease (MAFLD) through the activation of the PI3K/AKT/PDGF axis. Pck1-deficient male mice show hepatic lipid disorder, liver injury, and aggravated fibrosis and inflammation when on a high-fat diet. Forced expression of hepatic PCK1 ameliorates MAFLD [1]. In the context of non-alcoholic steatohepatitis (NASH) and hepatocellular carcinoma (HCC), combined knockdown of Ppara and Pck1 in vivo abolishes the beneficial outcomes of a 5:2 intermittent fasting regimen against inflammation and fibrosis, while overexpression of Pck1 alone or with Ppara lowers hepatic triglycerides and steatosis [2]. In diabetic nephropathy mouse models, Pck1 overexpression in proximal tubules protects against albuminuria, PT cell apoptosis, and mitoribosomal defects, while Pck1 deficiency leads to these adverse phenotypes [3].
In conclusion, Pck1 is essential for various biological functions including maintaining metabolic homeostasis, protecting against mitoribosomal defects, and regulating acid-base balance. Studies using Pck1 KO/CKO mouse models have revealed its crucial roles in diseases like MAFLD, NASH, HCC, and diabetic nephropathy, providing insights into potential therapeutic strategies for these conditions.
References:
1. Ye, Qian, Liu, Yi, Zhang, Guiji, Huang, Ailong, Tang, Ni. 2023. Deficiency of gluconeogenic enzyme PCK1 promotes metabolic-associated fatty liver disease through PI3K/AKT/PDGF axis activation in male mice. In Nature communications, 14, 1402. doi:10.1038/s41467-023-37142-3. https://pubmed.ncbi.nlm.nih.gov/36918564/
2. Gallage, Suchira, Ali, Adnan, Barragan Avila, Jose Efren, Karimi, Mohammad M, Heikenwalder, Mathias. 2024. A 5:2 intermittent fasting regimen ameliorates NASH and fibrosis and blunts HCC development via hepatic PPARα and PCK1. In Cell metabolism, 36, 1371-1393.e7. doi:10.1016/j.cmet.2024.04.015. https://pubmed.ncbi.nlm.nih.gov/38718791/
3. Hasegawa, Kazuhiro, Sakamaki, Yusuke, Tamaki, Masanori, Wakino, Shu. 2023. PCK1 Protects against Mitoribosomal Defects in Diabetic Nephropathy in Mouse Models. In Journal of the American Society of Nephrology : JASN, 34, 1343-1365. doi:10.1681/ASN.0000000000000156. https://pubmed.ncbi.nlm.nih.gov/37199399/
4. Xiang, Jin, Wang, Kai, Tang, Ni. 2022. PCK1 dysregulation in cancer: Metabolic reprogramming, oncogenic activation, and therapeutic opportunities. In Genes & diseases, 10, 101-112. doi:10.1016/j.gendis.2022.02.010. https://pubmed.ncbi.nlm.nih.gov/37013052/
5. Verissimo, Thomas, Dalga, Delal, Arnoux, Grégoire, Hall, Andrew M, de Seigneux, Sophie. 2023. PCK1 is a key regulator of metabolic and mitochondrial functions in renal tubular cells. In American journal of physiology. Renal physiology, 324, F532-F543. doi:10.1152/ajprenal.00038.2023. https://pubmed.ncbi.nlm.nih.gov/37102687/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen