Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6JCya-Pck1em1flox/Cya
Common Name:
Pck1-flox
Product ID:
S-CKO-04181
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Pck1-flox
Strain ID
CKOCMP-18534-Pck1-B6J-VA
Gene Name
Pck1
Product ID
S-CKO-04181
Gene Alias
PEPCK; PEPCK-C; Pck-1
Background
C57BL/6JCya
NCBI ID
18534
Modification
Conditional knockout
Chromosome
2
Phenotype
MGI:97501
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pck1em1flox/Cya mice (Catalog S-CKO-04181) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029017
NCBI RefSeq
NM_011044
Target Region
Exon 4~5
Size of Effective Region
~1.2 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Pck1, also known as phosphoenolpyruvate carboxykinase 1 (PEPCK1), is a key rate-limiting enzyme in gluconeogenesis, catalyzing the conversion of oxaloacetate to phosphoenolpyruvate [4]. It is involved in multiple biological processes and metabolic pathways, such as maintaining fasting glucose levels, affecting renal physiology, and being related to ammoniagenesis and cataplerosis in the liver [3,5]. Genetic models, especially KO and CKO mouse models, have been crucial in studying its functions.

In male mice, liver Pck1 deficiency promotes metabolic-associated fatty liver disease (MAFLD) through the activation of the PI3K/AKT/PDGF axis. Pck1-deficient male mice show hepatic lipid disorder, liver injury, and aggravated fibrosis and inflammation when on a high-fat diet. Forced expression of hepatic PCK1 ameliorates MAFLD [1]. In the context of non-alcoholic steatohepatitis (NASH) and hepatocellular carcinoma (HCC), combined knockdown of Ppara and Pck1 in vivo abolishes the beneficial outcomes of a 5:2 intermittent fasting regimen against inflammation and fibrosis, while overexpression of Pck1 alone or with Ppara lowers hepatic triglycerides and steatosis [2]. In diabetic nephropathy mouse models, Pck1 overexpression in proximal tubules protects against albuminuria, PT cell apoptosis, and mitoribosomal defects, while Pck1 deficiency leads to these adverse phenotypes [3].

In conclusion, Pck1 is essential for various biological functions including maintaining metabolic homeostasis, protecting against mitoribosomal defects, and regulating acid-base balance. Studies using Pck1 KO/CKO mouse models have revealed its crucial roles in diseases like MAFLD, NASH, HCC, and diabetic nephropathy, providing insights into potential therapeutic strategies for these conditions.

References:
1. Ye, Qian, Liu, Yi, Zhang, Guiji, Huang, Ailong, Tang, Ni. 2023. Deficiency of gluconeogenic enzyme PCK1 promotes metabolic-associated fatty liver disease through PI3K/AKT/PDGF axis activation in male mice. In Nature communications, 14, 1402. doi:10.1038/s41467-023-37142-3. https://pubmed.ncbi.nlm.nih.gov/36918564/
2. Gallage, Suchira, Ali, Adnan, Barragan Avila, Jose Efren, Karimi, Mohammad M, Heikenwalder, Mathias. 2024. A 5:2 intermittent fasting regimen ameliorates NASH and fibrosis and blunts HCC development via hepatic PPARα and PCK1. In Cell metabolism, 36, 1371-1393.e7. doi:10.1016/j.cmet.2024.04.015. https://pubmed.ncbi.nlm.nih.gov/38718791/
3. Hasegawa, Kazuhiro, Sakamaki, Yusuke, Tamaki, Masanori, Wakino, Shu. 2023. PCK1 Protects against Mitoribosomal Defects in Diabetic Nephropathy in Mouse Models. In Journal of the American Society of Nephrology : JASN, 34, 1343-1365. doi:10.1681/ASN.0000000000000156. https://pubmed.ncbi.nlm.nih.gov/37199399/
4. Xiang, Jin, Wang, Kai, Tang, Ni. 2022. PCK1 dysregulation in cancer: Metabolic reprogramming, oncogenic activation, and therapeutic opportunities. In Genes & diseases, 10, 101-112. doi:10.1016/j.gendis.2022.02.010. https://pubmed.ncbi.nlm.nih.gov/37013052/
5. Verissimo, Thomas, Dalga, Delal, Arnoux, Grégoire, Hall, Andrew M, de Seigneux, Sophie. 2023. PCK1 is a key regulator of metabolic and mitochondrial functions in renal tubular cells. In American journal of physiology. Renal physiology, 324, F532-F543. doi:10.1152/ajprenal.00038.2023. https://pubmed.ncbi.nlm.nih.gov/37102687/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest