C57BL/6JCya-Pdgfaem1flox/Cya
Common Name:
Pdgfa-flox
Product ID:
S-CKO-04216
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Pdgfa-flox
Strain ID
CKOCMP-18590-Pdgfa-B6J-VA
Gene Name
Product ID
S-CKO-04216
Gene Alias
PDGF-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pdgfaem1flox/Cya mice (Catalog S-CKO-04216) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000076095
NCBI RefSeq
NM_008808
Target Region
Exon 3~4
Size of Effective Region
~2.8 kb
Detailed Document
Overview of Gene Research
Pdgfa, short for Platelet-derived growth factor subunit A, is a key member of the PDGF family. It plays crucial roles in various biological processes such as cell growth, angiogenesis, and tissue repair. It exerts its functions mainly through binding to its receptor PDGFRA, and is involved in multiple signaling pathways like the AKT pathway, which is associated with cell survival and proliferation [1,3].
In glioblastoma, while PDGFRA has long been considered as the receptor for PDGFA, new research shows that PDGFA can also activate EPHA2 independently of PDGFRA and AKT activation. Co-upregulation of PDGFRA and EPHA2 leads to worse patient prognosis and poorer therapeutic effects. Inhibiting both PDGFRA and EPHA2 shows synergetic inhibitory effects on glioma cells [1].
In esophageal squamous cell carcinoma, high expression of PDGFA is positively correlated with T stage and is an independent predictor for poor prognosis [4].
In biliary atresia, the G allele of rs9690350 in PDGFA is significantly associated with an increased risk of the disease [2].
In conclusion, Pdgfa is involved in multiple disease-related biological processes. Its study through gene-related models has provided insights into diseases such as glioblastoma, esophageal squamous cell carcinoma, and biliary atresia. Understanding the functions of Pdgfa can potentially contribute to the development of new therapeutic strategies for these diseases.
References：
1. Gai, Qu-Jing, Fu, Zhen, He, Jiang, Bian, Xiu-Wu, Wang, Yan. 2022. EPHA2 mediates PDGFA activity and functions together with PDGFRA as prognostic marker and therapeutic target in glioblastoma. In Signal transduction and targeted therapy, 7, 33. doi:10.1038/s41392-021-00855-2. https://pubmed.ncbi.nlm.nih.gov/35105853/
2. Liu, Fei, Zeng, Jixiao, Zhu, Deli, Zhang, Yan, Zhang, Ruizhong. . PDGFA gene rs9690350 polymorphism increases biliary atresia risk in Chinese children. In Bioscience reports, 40, . doi:10.1042/BSR20200068. https://pubmed.ncbi.nlm.nih.gov/32662506/
3. Xu, Ran, Ji, Jianxiong, Zhang, Xin, Wang, Jian, Li, Xingang. 2017. PDGFA/PDGFRα-regulated GOLM1 promotes human glioma progression through activation of AKT. In Journal of experimental & clinical cancer research : CR, 36, 193. doi:10.1186/s13046-017-0665-3. https://pubmed.ncbi.nlm.nih.gov/29282077/
4. Han, Na, Zhang, Yan-Yan, Zhang, Zhong-Mian, Zhang, Yi-Bing, Zhao, Wen-Chao. . High expression of PDGFA predicts poor prognosis of esophageal squamous cell carcinoma. In Medicine, 100, e25932. doi:10.1097/MD.0000000000025932. https://pubmed.ncbi.nlm.nih.gov/34011067/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen