C57BL/6JCya-Pdha1em1flox/Cya
Common Name:
Pdha1-flox
Product ID:
S-CKO-04220
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Pdha1-flox
Strain ID
CKOCMP-18597-Pdha1-B6J-VA
Gene Name
Product ID
S-CKO-04220
Gene Alias
Pdha-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
X
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pdha1em1flox/Cya mice (Catalog S-CKO-04220) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000033662
NCBI RefSeq
NM_008810
Target Region
Exon 3~4
Size of Effective Region
~3.1 kb
Detailed Document
Overview of Gene Research
Pdha1, also known as pyruvate dehydrogenase E1 component subunit alpha, is a key enzyme in the tricarboxylic acid cycle. It links glycolysis to the mitochondrial citric acid cycle by catalyzing the conversion of pyruvate to acetyl-CoA, thus playing a crucial role in energy metabolism [1-6].
In sepsis-induced acute kidney injury (SAKI), down-regulation of Sirtuin 3 (SIRT3) leads to PDHA1 hyperacetylation and inactivation, causing lactate overproduction [1]. In cholangiocarcinoma, succinylation of PDHA1 lysine 83 changes PDH enzyme activity, modulates metabolic flux, and promotes immune escape [2]. In colorectal cancer, RNF4-mediated degradation of PDHA1 promotes glycolytic metabolism, proliferation, and metastasis [3]. In cervical cancer, AP2α negatively regulates PDHA1 to promote aggressive features and aerobic glycolysis [4].
In conclusion, Pdha1 is essential for energy metabolism, connecting glycolysis to the citric acid cycle. Studies using various models, including those related to SAKI, cholangiocarcinoma, colorectal cancer, and cervical cancer, have revealed its significance in disease-related metabolic reprogramming and disease progression. Understanding Pdha1 provides insights into disease mechanisms and potential therapeutic targets in these disease areas.
References:
1. An, Sheng, Yao, Yi, Hu, Hongbin, Chen, Zhongqing, Zeng, Zhenhua. 2023. PDHA1 hyperacetylation-mediated lactate overproduction promotes sepsis-induced acute kidney injury via Fis1 lactylation. In Cell death & disease, 14, 457. doi:10.1038/s41419-023-05952-4. https://pubmed.ncbi.nlm.nih.gov/37479690/
2. Zhang, Ning, Sun, Linmao, Zhou, Shuo, Liu, Yao, Liu, Lianxin. 2025. Cholangiocarcinoma PDHA1 succinylation suppresses macrophage antigen presentation via alpha-ketoglutaric acid accumulation. In Nature communications, 16, 3177. doi:10.1038/s41467-025-58429-7. https://pubmed.ncbi.nlm.nih.gov/40180922/
3. Chen, Jierong, Li, Zi-Yue, Zheng, Guansheng, Gu, Bing, Yue, Cai-Feng. 2024. RNF4 mediated degradation of PDHA1 promotes colorectal cancer metabolism and metastasis. In NPJ precision oncology, 8, 258. doi:10.1038/s41698-024-00724-5. https://pubmed.ncbi.nlm.nih.gov/39521913/
4. Zhao, Lijie, Geng, Rong, Huang, Yi, Zhou, Cankun, Wang, Yifeng. 2023. AP2α negatively regulates PDHA1 in cervical cancer cells to promote aggressive features and aerobic glycolysis in vitro and in vivo. In Journal of gynecologic oncology, 34, e59. doi:10.3802/jgo.2023.34.e59. https://pubmed.ncbi.nlm.nih.gov/37055163/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen