C57BL/6JCya-Pemtem1flox/Cya
Common Name
Pemt-flox
Product ID
S-CKO-04237
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-18618-Pemt-B6J-VA
Status
When using this mouse strain in a publication, please cite “Pemt-flox Mouse (Catalog S-CKO-04237) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Pemt-flox
Strain ID
CKOCMP-18618-Pemt-B6J-VA
Gene Name
Product ID
S-CKO-04237
Gene Alias
PLMT, PEAMT, PEMT2, Pempt, Pempt2
Background
C57BL/6JCya
Gene Full Name
phosphatidylethanolamine N-methyltransferase
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 11 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000102692
NCBI Transcript ID
NM_008819
Target Region
Exon 3
Size of Effective Region
~1.2 kb
Overview of Gene Research
Pemt, or phosphatidylethanolamine N-methyltransferase, is an enzyme that catalyzes the production of phosphatidylcholine. This process is crucial for lipid metabolism, as phosphatidylcholine is an important component of cell membranes. The non-canonical synthesis pathway involving Pemt is part of the overall lipid-related biological pathways [2].
Reduced expression of hepatic Pemt in mice causes steatosis, inflammation, and fibrosis, suggesting its key role in maintaining liver health [1]. In HCV-infected cells, Pemt is induced, mediating steatosis and supporting virus replication. Knocking down Pemt with siRNA inhibits virus replication and reduces lipid content in these cells [2]. Also, in humans, Pemt rs7946 polymorphism exerts sex-specific effects on choline requirement and hepatic steatosis risk. For example, in postmenopausal Taiwanese women with the Pemt rs7946 GG genotype, current dietary choline intake may be inadequate to minimize hepatic steatosis risk, while in older men, high choline intake may increase the risk of non-alcoholic fatty liver disease [3].
In conclusion, Pemt is essential for lipid metabolism, especially in processes related to phosphatidylcholine synthesis. Its role in liver-related diseases such as non-alcoholic fatty liver disease and HCV-induced steatosis is significant. The study of Pemt using mouse models and human genetic polymorphism analysis helps to understand its function in maintaining normal physiological conditions and the pathogenesis of related diseases.
References：
1. Piras, Ignazio S, Raju, Anish, Don, Janith, Gerhard, Glenn S, DiStefano, Johanna K. 2022. Hepatic PEMT Expression Decreases with Increasing NAFLD Severity. In International journal of molecular sciences, 23, . doi:10.3390/ijms23169296. https://pubmed.ncbi.nlm.nih.gov/36012560/
2. Abomughaid, Mosleh, Tay, Enoch S E, Pickford, Russell, George, Jacob, Douglas, Mark W. 2023. PEMT Mediates Hepatitis C Virus-Induced Steatosis, Explains Genotype-Specific Phenotypes and Supports Virus Replication. In International journal of molecular sciences, 24, . doi:10.3390/ijms24108781. https://pubmed.ncbi.nlm.nih.gov/37240132/
3. Wu, Chien-Hsien, Chang, Ting-Yu, Chen, Yen-Chu, Huang, Rwei-Fen S. 2023. PEMT rs7946 Polymorphism and Sex Modify the Effect of Adequate Dietary Choline Intake on the Risk of Hepatic Steatosis in Older Patients with Metabolic Disorders. In Nutrients, 15, . doi:10.3390/nu15143211. https://pubmed.ncbi.nlm.nih.gov/37513629/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
