C57BL/6JCya-Pik3cdem1flox/Cya
Common Name
Pik3cd-flox
Product ID
S-CKO-04276
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-18707-Pik3cd-B6J-VA
Status
When using this mouse strain in a publication, please cite “Pik3cd-flox Mouse (Catalog S-CKO-04276) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Pik3cd-flox
Strain ID
CKOCMP-18707-Pik3cd-B6J-VA
Gene Name
Product ID
S-CKO-04276
Gene Alias
p110delta, 2410099E07Rik, 2610208K16Rik
Background
C57BL/6JCya
Gene Full Name
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 4 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000105690
NCBI Transcript ID
NM_001164049
Target Region
Exon 12~20
Size of Effective Region
~2.9 kb
Overview of Gene Research
Pik3cd, encoding the catalytic p110δ subunit of phosphoinositide 3-kinase (PI3K), is crucial in the PI3K-AKT-mechanistic target of rapamycin pathway. This pathway is involved in various biological processes such as cell growth, differentiation, and immune response [2,7].
In breast carcinoma, Pik3cd expression is associated with prognosis, epithelial-mesenchymal transition (EMT), and tumor immune infiltration. In gastric carcinoma, its overexpression promotes tumor development, and genetic silencing retards proliferation and migration both in vitro and in vivo [1,4]. In glioblastoma, knockout of Pik3cd in cell lines reduces migration, invasion, and colony-forming ability, and negates tumorigenesis in nude mice, potentially acting through cytoskeletal proteins PAK3 and PLEK2 [5]. In colorectal cancer, overexpression of Pik3cd promotes cell growth, migration, and invasion by activating AKT/GSK-3β/β-catenin signaling [6]. In abnormal retinal angiogenesis, editing genomic Pik3cd in a mouse model significantly reduces pathological angiogenesis [3]. In addition, in diabetic retinopathy, Pik3cd is involved in promoting microvascular endothelial cell proliferation, migration, and angiogenesis [8].
In conclusion, Pik3cd plays essential roles in multiple biological processes and disease conditions. Studies using gene knockout models, such as in glioblastoma and abnormal retinal angiogenesis mouse models, have revealed its role in tumorigenesis and angiogenesis. These findings suggest that Pik3cd could be a potential therapeutic target for various diseases including carcinomas, glioblastoma, and diseases related to abnormal angiogenesis [3,5].
References：
1. He, Wenxing, Zhang, Haoyi, Cheng, Hong, Wen, Jianfeng, Li, Dongmei. 2023. PIK3CD correlates with prognosis, epithelial-mesenchymal transition and tumor immune infiltration in breast carcinoma. In Discover oncology, 14, 187. doi:10.1007/s12672-023-00805-0. https://pubmed.ncbi.nlm.nih.gov/37861728/
2. Lucas, Carrie L, Chandra, Anita, Nejentsev, Sergey, Condliffe, Alison M, Okkenhaug, Klaus. 2016. PI3Kδ and primary immunodeficiencies. In Nature reviews. Immunology, 16, 702-714. doi:10.1038/nri.2016.93. https://pubmed.ncbi.nlm.nih.gov/27616589/
3. Wu, Wenyi, Ma, Gaoen, Qi, Hui, Zhang, Shaochong, Lei, Hetian. . Genome Editing of Pik3cd Impedes Abnormal Retinal Angiogenesis. In Human gene therapy, 34, 30-41. doi:10.1089/hum.2022.079. https://pubmed.ncbi.nlm.nih.gov/36515172/
4. Hu, Qingqing, Dou, Ning, Wu, Qiong, Li, Yandong, Chen, Jingde. 2024. JAK3/STAT5 signaling-triggered upregulation of PIK3CD contributes to gastric carcinoma development. In Journal of cell communication and signaling, 18, e12017. doi:10.1002/ccs3.12017. https://pubmed.ncbi.nlm.nih.gov/38545256/
5. Shao, Wei, Azam, Zulfikar, Guo, Jintao, To, Shing Shun Tony. 2022. Oncogenic potential of PIK3CD in glioblastoma is exerted through cytoskeletal proteins PAK3 and PLEK2. In Laboratory investigation; a journal of technical methods and pathology, 102, 1314-1322. doi:10.1038/s41374-022-00821-8. https://pubmed.ncbi.nlm.nih.gov/35851857/
6. Chen, Jing-Song, Huang, Jiong-Qiang, Luo, Bing, Wen, Guang-Ming, Zhong, Jian-Feng. 2019. PIK3CD induces cell growth and invasion by activating AKT/GSK-3β/β-catenin signaling in colorectal cancer. In Cancer science, 110, 997-1011. doi:10.1111/cas.13931. https://pubmed.ncbi.nlm.nih.gov/30618098/
7. Edwards, Emily S J, Bier, Julia, Cole, Theresa S, Uzel, Gulbu, Tangye, Stuart G. 2018. Activating PIK3CD mutations impair human cytotoxic lymphocyte differentiation and function and EBV immunity. In The Journal of allergy and clinical immunology, 143, 276-291.e6. doi:10.1016/j.jaci.2018.04.030. https://pubmed.ncbi.nlm.nih.gov/29800648/
8. He, Fu-Tao, Fu, Xiao-Lin, Li, Mo-Han, Fu, Chun-Yan, Chen, Jian-Zhi. 2023. USP14 Regulates ATF2/PIK3CD Axis to Promote Microvascular Endothelial Cell Proliferation, Migration, and Angiogenesis in Diabetic Retinopathy. In Biochemical genetics, 61, 2076-2091. doi:10.1007/s10528-023-10358-0. https://pubmed.ncbi.nlm.nih.gov/36939972/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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