C57BL/6NCya-Pkmem1flox/Cya
Common Name:
Pkm-flox
Product ID:
S-CKO-04295
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Pkm-flox
Strain ID
CKOCMP-18746-Pkm-B6N-VA
Gene Name
Product ID
S-CKO-04295
Gene Alias
Pk-2; Pk-3; Pk3; Pkm2
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Pkmem1flox/Cya mice (Catalog S-CKO-04295) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000034834
NCBI RefSeq
NM_011099
Target Region
Exon 4~6
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
Pkm, which codes for pyruvate kinase muscle isoforms, is a key gene in the glycolytic pathway. The PKM gene produces PKM1 and PKM2 through alternative splicing, with PKM2 being upregulated in most cancers and playing a crucial role in the Warburg effect, where cancer cells prefer aerobic glycolysis over oxidative phosphorylation for energy metabolism [1,2,3,5].
In hepatocellular carcinoma (HCC), antisense oligonucleotides (ASO) that switch PKM splicing from PKM2 to PKM1 inhibit tumor growth both in vitro and in vivo. In an orthotopic HCC xenograft mouse model, lead ASOs targeting PKM inhibited tumor growth. In a genetic HCC mouse model, a mouse-specific ASO induced Pkm splice switching and inhibited tumorigenesis without observable toxicity [1]. In a carotid artery injury model, Phb2 deficiency in vascular smooth muscle cells (VSMCs) facilitated PKM1/2 mRNA splicing to PKM2, enhanced glycolysis, and aggravated post-injury neointima formation. This shows that counteracting PKM2 splicing can maintain the contractile phenotype of VSMCs [4].
In conclusion, Pkm is essential in regulating glycolysis and its alternative splicing products, especially PKM2, are closely associated with cancer development. Mouse models, such as the genetic HCC mouse model and the carotid artery injury model, have been crucial in revealing the role of Pkm in cancer and vascular diseases, providing potential therapeutic targets for these conditions.
References:
1. Ma, Wai Kit, Voss, Dillon M, Scharner, Juergen, Bennett, C Frank, Krainer, Adrian R. . ASO-Based PKM Splice-Switching Therapy Inhibits Hepatocellular Carcinoma Growth. In Cancer research, 82, 900-915. doi:10.1158/0008-5472.CAN-20-0948. https://pubmed.ncbi.nlm.nih.gov/34921016/
2. Li, Yuchao, Zhang, Shuwei, Li, Yuexian, Zang, Wenli, Pan, Yaping. 2024. The Regulatory Network of hnRNPs Underlying Regulating PKM Alternative Splicing in Tumor Progression. In Biomolecules, 14, . doi:10.3390/biom14050566. https://pubmed.ncbi.nlm.nih.gov/38785973/
3. Rong, Shikuo, Dai, Bao, Yang, Chunrong, Chen, Jian, Wu, Zeyu. 2024. HNRNPC modulates PKM alternative splicing via m6A methylation, upregulating PKM2 expression to promote aerobic glycolysis in papillary thyroid carcinoma and drive malignant progression. In Journal of translational medicine, 22, 914. doi:10.1186/s12967-024-05668-9. https://pubmed.ncbi.nlm.nih.gov/39380010/
4. Jia, Yiting, Mao, Chenfeng, Ma, Zihan, Fu, Yi, Kong, Wei. 2022. PHB2 Maintains the Contractile Phenotype of VSMCs by Counteracting PKM2 Splicing. In Circulation research, 131, 807-824. doi:10.1161/CIRCRESAHA.122.321005. https://pubmed.ncbi.nlm.nih.gov/36200440/
5. Zahra, Kulsoom, Dey, Tulika, Mishra, Surendra Pratap, Pandey, Uma. 2020. Pyruvate Kinase M2 and Cancer: The Role of PKM2 in Promoting Tumorigenesis. In Frontiers in oncology, 10, 159. doi:10.3389/fonc.2020.00159. https://pubmed.ncbi.nlm.nih.gov/32195169/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen