C57BL/6JCya-Prkacaem1flox/Cya
Common Name:
Prkaca-flox
Product ID:
S-CKO-04299
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Prkaca-flox
Strain ID
CKOCMP-18747-Prkaca-B6J-VA
Gene Name
Product ID
S-CKO-04299
Gene Alias
PKCD; Pkaca
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Prkacaem1flox/Cya mice (Catalog S-CKO-04299) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000005606
NCBI RefSeq
NM_008854
Target Region
Exon 4~5
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
Prkaca, encoding the catalytic subunit α of protein kinase A (PKA), is a key player in the cAMP signaling pathway. PKA, as a holoenzyme, consists of a regulatory subunit dimer and two catalytic subunits. When cAMP binds to the regulatory subunits, the catalytic subunits are released to phosphorylate downstream targets, thus propagating cAMP-responsive cell signaling events [5]. This process is crucial for various biological functions in all tissues [5].
In diseases, Prkaca has been implicated in multiple conditions. Somatic mutations in Prkaca are the most frequently altered gene in cortisol-producing adenomas, with patients carrying these mutations showing a more severe phenotype and being identified at a younger age [3]. Germline Prkaca amplification has been associated with primary pigmented nodular adrenocortical disease (PPNAD), a rare adrenocorticotropin hormone (ACTH)-independent Cushing's syndrome [2]. Also, the DNAJB1-Prkaca fusion transcript is the oncogenic driver in fibrolamellar hepatocellular carcinoma, and peptide-based immunotherapy targeting this fusion protein has shown efficacy in a single-patient study [1]. Moreover, somatic Prkaca mutations may be correlated with the upregulation of LHCGR, which drives the accelerated growth of co-secretion tumors during pregnancy [4].
In conclusion, Prkaca is essential for cAMP-dependent protein kinase function and cell signaling. Its dysregulation, whether through somatic mutations, germline amplification, or gene fusion, is associated with various adrenocortical and liver-related diseases. Understanding Prkaca's role through research on these disease-associated alterations can potentially lead to new therapeutic strategies for these conditions.
References:
1. Bauer, Jens, Köhler, Natalie, Maringer, Yacine, Hailfinger, Stephan, Walz, Juliane S. 2022. The oncogenic fusion protein DNAJB1-PRKACA can be specifically targeted by peptide-based immunotherapy in fibrolamellar hepatocellular carcinoma. In Nature communications, 13, 6401. doi:10.1038/s41467-022-33746-3. https://pubmed.ncbi.nlm.nih.gov/36302754/
2. Yang, Wang-Rong, Liang, Xing-Huan, Qin, Ying-Fen, Liu, Yu-Ping, Luo, Zuo-Jie. . Germline PRKACA amplification-associated primary pigmented nodular adrenocortical disease: a case report and literature review. In Archives of endocrinology and metabolism, 68, e220491. doi:10.20945/2359-4292-2022-0491. https://pubmed.ncbi.nlm.nih.gov/37988664/
3. Dalmazi, G D, Beuschlein, F. 2016. PRKACA Mutations in Adrenal Adenomas: Genotype/Phenotype Correlations. In Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme, 49, 301-306. doi:10.1055/s-0042-120416. https://pubmed.ncbi.nlm.nih.gov/27871112/
4. Lin, Jianfan, Li, Yufei, Huang, Zhenxing, Lu, Decheng, Luo, Zuojie. 2024. Rare correlation of somatic PRKACA mutations with pregnancy-associated aldosterone- and cortisol-producing adenomas: a case report and literature review. In BMC endocrine disorders, 24, 116. doi:10.1186/s12902-024-01645-x. https://pubmed.ncbi.nlm.nih.gov/39010034/
5. Turnham, Rigney E, Scott, John D. 2015. Protein kinase A catalytic subunit isoform PRKACA; History, function and physiology. In Gene, 577, 101-8. doi:10.1016/j.gene.2015.11.052. https://pubmed.ncbi.nlm.nih.gov/26687711/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen