C57BL/6JCya-Pla2g1bem1flox/Cya
Common Name:
Pla2g1b-flox
Product ID:
S-CKO-04320
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Pla2g1b-flox
Strain ID
CKOCMP-18778-Pla2g1b-B6J-VA
Gene Name
Product ID
S-CKO-04320
Gene Alias
Pla2a; sPLA2IB
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pla2g1bem1flox/Cya mice (Catalog S-CKO-04320) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000031495
NCBI RefSeq
NM_011107
Target Region
Exon 2
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Pla2g1b, or group 1B phospholipase A2, is a secreted enzyme. It catalyzes the hydrolytic removal of the sn-2 fatty acyl moiety from phospholipids, playing a key role in lipid metabolism. It is mainly synthesized in the pancreas and also expressed in the lung. Involved pathways include α-linolenic acid metabolism and arachidonic acid metabolism. It has importance in metabolic, inflammatory processes, and anti-helminth responses [5].
In mouse models, Pla2g1b-/-mice showed lower postprandial glycemia due to enhanced postprandial glucose uptake by liver, heart, and muscle tissues and altered hepatic glucose metabolism. This was attributed to a decrease in dietary phospholipids absorbed as lysophospholipids, indicating its role in postprandial hyperglycemia regulation [6].
In addition, in vitro studies suggest that PLA2G1B may modulate lung inflammation. Also, it has been associated with various diseases. In HIV-infected patients, PLA2G1B synergizes with HIV gp41 to induce CD4 + T-cell anergy and may contribute to CD4 lymphopenia [2,3]. Bioinformatics and machine learning analysis indicate that PLA2G1B gene may be a preventive gene for lung cancer [1]. Genetic analysis in patients with non-alcoholic chronic pancreatitis suggests that genetic alterations in PLA2G1B do not predispose to the development of this disease [4].
In conclusion, Pla2g1b is crucial for lipid metabolism-related processes like postprandial glucose regulation. Its role in diseases such as HIV-related immunodeficiency and potential preventive role in lung cancer are areas of significant interest. The use of gene-knockout mouse models has been instrumental in understanding its functions in metabolic and disease-related contexts.
References:
1. Guan, Shuhong, Xu, Zhanzhan, Yang, Tingting, Liu, Huimin, Zhou, Jun. 2024. Identifying potential targets for preventing cancer progression through the PLA2G1B recombinant protein using bioinformatics and machine learning methods. In International journal of biological macromolecules, 276, 133918. doi:10.1016/j.ijbiomac.2024.133918. https://pubmed.ncbi.nlm.nih.gov/39019365/
2. Pothlichet, Julien, Rose, Thierry, Bugault, Florence, Lambeau, Gérard, Thèze, Jacques. . PLA2G1B is involved in CD4 anergy and CD4 lymphopenia in HIV-infected patients. In The Journal of clinical investigation, 130, 2872-2887. doi:10.1172/JCI131842. https://pubmed.ncbi.nlm.nih.gov/32436864/
3. Pothlichet, Julien, Meola, Annalisa, Bugault, Florence, Sauvanet, Alain, Thèze, Jacques. 2022. Microbial Protein Binding to gC1qR Drives PLA2G1B-Induced CD4 T-Cell Anergy. In Frontiers in immunology, 13, 824746. doi:10.3389/fimmu.2022.824746. https://pubmed.ncbi.nlm.nih.gov/35392090/
4. Ewers, Maren, Epple, Denise, Bugert, Peter, Rosendahl, Jonas, Witt, Heiko. 2022. Genetic analysis of pancreatic phospholipase A2 (PLA2G1B) in patients with chronic pancreatitis. In Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.], 22, 244-247. doi:10.1016/j.pan.2022.01.003. https://pubmed.ncbi.nlm.nih.gov/35031208/
5. Hui, David Y. 2018. Group 1B phospholipase A2 in metabolic and inflammatory disease modulation. In Biochimica et biophysica acta. Molecular and cell biology of lipids, 1864, 784-788. doi:10.1016/j.bbalip.2018.07.001. https://pubmed.ncbi.nlm.nih.gov/30003964/
6. Labonté, Eric D, Kirby, R Jason, Schildmeyer, Nicholas M, Huggins, Kevin W, Hui, David Y. . Group 1B phospholipase A2-mediated lysophospholipid absorption directly contributes to postprandial hyperglycemia. In Diabetes, 55, 935-41. doi:. https://pubmed.ncbi.nlm.nih.gov/16567514/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen