C57BL/6JCya-Ppp5cem1flox/Cya
Common Name:
Ppp5c-flox
Product ID:
S-CKO-04423
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ppp5c-flox
Strain ID
CKOCMP-19060-Ppp5c-B6J-VA
Gene Name
Product ID
S-CKO-04423
Gene Alias
PP5
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ppp5cem1flox/Cya mice (Catalog S-CKO-04423) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000003183
NCBI RefSeq
NM_011155
Target Region
Exon 2
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Ppp5c, encoding protein phosphatase 5 catalytic subunit, is a serine/threonine protein phosphatase. It has dual functions of dephosphorylation and co-chaperone, participating in many signal transductions related to cell growth, differentiation, and responses to hormonal signals or cellular stress [1]. It is involved in various pathways such as Wnt signalling by dephosphorylating Dvl2 [2], and endothelial calcium signaling regulation in concert with FKBP51 and S100A6 [3].
Knockdown of Ppp5c in prostate cancer cells inhibited cell proliferation and colony formation, arrested cells in G0/G1 phase and induced apoptosis, with JNK and ERK phosphorylation promoted [4]. In hepatocellular carcinoma cells, Ppp5c knockdown suppressed proliferation and colony formation ability, leading to G0/G1 and G2/M phase arrest [6]. A de novo missense variant in Ppp5c in a patient was associated with microcephaly, developmental delay, and refractory epilepsy, and functional evaluation in C. elegans suggested the variant was likely damaging [5].
In conclusion, Ppp5c is crucial for cell growth-related processes. Its dysregulation is linked to diseases like cancer, epilepsy, and neurological disorders. Studies on Ppp5c, including through knockdown and variant functional analysis, help in understanding its role in these biological processes and disease conditions, potentially providing new directions for disease diagnosis and treatment.
References:
1. Zhang, Hengheng, Zhang, Qiuyue, Tu, Jiaqi, You, Qidong, Wang, Lei. 2023. Dual function of protein phosphatase 5 (PPP5C): An emerging therapeutic target for drug discovery. In European journal of medicinal chemistry, 254, 115350. doi:10.1016/j.ejmech.2023.115350. https://pubmed.ncbi.nlm.nih.gov/37054560/
2. Xie, Jianlei, Han, Meng, Zhang, Miaojun, Deng, Haiteng, Wu, Wei. 2018. PP5 (PPP5C) is a phosphatase of Dvl2. In Scientific reports, 8, 2715. doi:10.1038/s41598-018-21124-3. https://pubmed.ncbi.nlm.nih.gov/29426949/
3. Haldar, Barnita, Hamilton, Caleb L, Solodushko, Viktoriya, Scammell, Jonathan G, Cioffi, Donna L. 2020. S100A6 is a positive regulator of PPP5C-FKBP51-dependent regulation of endothelial calcium signaling. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34, 3179-3196. doi:10.1096/fj.201901777R. https://pubmed.ncbi.nlm.nih.gov/31916625/
4. Lv, Jian-Min, Chen, Lu, Gao, Yi, Cui, Xin-Gang, Xu, Dan-Feng. 2018. PPP5C promotes cell proliferation and survival in human prostate cancer by regulating of the JNK and ERK1/2 phosphorylation. In OncoTargets and therapy, 11, 5797-5809. doi:10.2147/OTT.S161280. https://pubmed.ncbi.nlm.nih.gov/30254472/
5. Fielder, Sara M, Rosenfeld, Jill A, Burrage, Lindsay C, Schedl, Tim, Pak, Stephen C. 2022. Functional analysis of a novel de novo variant in PPP5C associated with microcephaly, seizures, and developmental delay. In Molecular genetics and metabolism, 136, 65-73. doi:10.1016/j.ymgme.2022.03.007. https://pubmed.ncbi.nlm.nih.gov/35361529/
6. Feng, Liang, Sun, Peng, Li, Zhiyu, Liu, Ming, Sun, Shibo. 2014. Knockdown of PPP5C inhibits growth of hepatocellular carcinoma cells in vitro. In Applied biochemistry and biotechnology, 175, 526-34. doi:10.1007/s12010-014-1281-8. https://pubmed.ncbi.nlm.nih.gov/25326185/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen