C57BL/6JCya-Procrem1flox/Cya
Common Name
Procr-flox
Product ID
S-CKO-04459
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-19124-Procr-B6J-VA
Status
When using this mouse strain in a publication, please cite “Procr-flox Mouse (Catalog S-CKO-04459) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Procr-flox
Strain ID
CKOCMP-19124-Procr-B6J-VA
Gene Name
Product ID
S-CKO-04459
Gene Alias
Ccca, Epcr, Ccd41
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 2
Phenotype
Datasheet
Application
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Strain Description
Ensembl Number
ENSMUST00000029140
NCBI RefSeq
NM_011171
Target Region
Exon 3~4
Size of Effective Region
~2.9 kb
Overview of Gene Research
Procr, also known as Protein C receptor, functions as a signaling receptor and is crucial for the maintenance and self-renewal of various stem cells [2]. It has been implicated in multiple biological processes and is associated with pathways like the interaction with heat shock protein 90 (HSP90AA1), Src, and IGF1R upon ligand (protein C) stimulation [2]. Procr-expressing cells have been identified as progenitors in several tissues, highlighting its overall biological importance in tissue homeostasis and repair [1,2,4,5]. Genetic models, especially KO/CKO mouse models, are valuable for studying Procr.
In KO/CKO mouse models, conditional knockout of Procr in mammary stem cells (MaSCs) demonstrated its essentiality for mammary gland development and homeostasis [2]. Ablation of Procr+ cells in the ovarian surface epithelium (OSE) impeded the repair process after ovulatory rupture, indicating Procr+ cells' critical role in OSE repair [4]. In the pancreas, Procr+ cells were shown to be endocrine progenitors capable of long-term expansion into islet-like organoids, and these organoids could reverse diabetes when transplanted into diabetic mice [1,3].
In conclusion, Procr is essential for the maintenance and self-renewal of stem cells in various tissues, playing a significant role in processes such as tissue repair, organ development, and islet-related functions. Studies using Procr KO/CKO mouse models have provided insights into its functions in mammary gland development, ovarian epithelium repair, and pancreatic islet-related diseases, contributing to a better understanding of these biological processes and potential therapeutic strategies.
References：
1. Wang, Daisong, Wang, Jingqiang, Bai, Lanyue, Clevers, Hans, Zeng, Yi Arial. . Long-Term Expansion of Pancreatic Islet Organoids from Resident Procr+ Progenitors. In Cell, 180, 1198-1211.e19. doi:10.1016/j.cell.2020.02.048. https://pubmed.ncbi.nlm.nih.gov/32200801/
2. Liu, Chunye, Lin, Changdong, Wang, Daisong, Chen, Jianfeng, Zeng, Yi Arial. . Procr functions as a signaling receptor and is essential for the maintenance and self-renewal of mammary stem cells. In Cell reports, 38, 110548. doi:10.1016/j.celrep.2022.110548. https://pubmed.ncbi.nlm.nih.gov/35320720/
3. Wang, Jingqiang, Wang, Daisong, Chen, Xinyi, Liu, Chunye, Zeng, Yi Arial. 2022. Isolation of mouse pancreatic islet Procr+ progenitors and long-term expansion of islet organoids in vitro. In Nature protocols, 17, 1359-1384. doi:10.1038/s41596-022-00683-w. https://pubmed.ncbi.nlm.nih.gov/35396545/
4. Wang, Jingqiang, Wang, Daisong, Chu, Kun, Li, Wen, Zeng, Yi Arial. 2019. Procr-expressing progenitor cells are responsible for murine ovulatory rupture repair of ovarian surface epithelium. In Nature communications, 10, 4966. doi:10.1038/s41467-019-12935-7. https://pubmed.ncbi.nlm.nih.gov/31672973/
5. Wang, Jingqiang, Liu, Chunye, He, Lingli, Zhang, Lei, Zeng, Yi Arial. 2022. Selective YAP activation in Procr cells is essential for ovarian stem/progenitor expansion and epithelium repair. In eLife, 11, . doi:10.7554/eLife.75449. https://pubmed.ncbi.nlm.nih.gov/35285801/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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