C57BL/6JCya-Psmb4em1flox/Cya
Common Name:
Psmb4-flox
Product ID:
S-CKO-04488
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Psmb4-flox
Strain ID
CKOCMP-19172-Psmb4-B6J-VA
Gene Name
Product ID
S-CKO-04488
Gene Alias
Pros-27
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Psmb4em1flox/Cya mice (Catalog S-CKO-04488) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000005923
NCBI RefSeq
NM_008945
Target Region
Exon 1~4
Size of Effective Region
~3.7 kb
Detailed Document
Overview of Gene Research
Psmb4, the proteasome subunit beta type 4, is a non-catalytic subunit for the proteasome assembly. The proteasome system is crucial for protein degradation in cells, and Psmb4 likely contributes to the proper function of this system, which is involved in various cellular processes such as cell cycle regulation, apoptosis, and immune responses [4].
Psmb4 has been found to interact with viral proteins. It can interact with the NS1 protein of influenza A virus (IAV), potentially facilitating its degradation and thus suppressing IAV replication [1]. Psmb4 also interacts with the Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Nsp1α protein, mediating its K63-linked ubiquitination and autolysosomal degradation, and activating the NF-κB signaling pathway to produce type I interferons, restricting PRRSV replication [2]. In the context of cancer, knockdown of Psmb4 significantly inhibits cervical cancer cell proliferation [4]. In epithelial ovarian cancer (EOC), Psmb4 is highly expressed, and its knockdown reduces cell proliferation and NF-κB activity [5]. In bladder cancer, reducing Psmb4 inhibits the angiogenesis and migration of endothelial cells [6]. Regarding myocardial ischemia/reperfusion injury, Psmb4 inhibits cardiomyocyte apoptosis via activating the NF-κB signaling pathway [3].
In conclusion, Psmb4 plays diverse and important roles in multiple biological processes and diseases. Its interactions with viral proteins suggest a role in antiviral defense, while its involvement in cancer cell proliferation and apoptosis in different cancer types, as well as its function in myocardial ischemia/reperfusion injury, indicates its potential as a therapeutic target in these disease areas. The study of Psmb4 using genetic models helps to better understand its functions and provides insights for developing new treatment strategies.
References:
1. Yang, Chee-Hing, Hsu, Che-Fang, Lai, Xiang-Qing, Li, Hui-Chun, Lo, Shih-Yen. 2022. Cellular PSMB4 Protein Suppresses Influenza A Virus Replication through Targeting NS1 Protein. In Viruses, 14, . doi:10.3390/v14102277. https://pubmed.ncbi.nlm.nih.gov/36298834/
2. Yi, Heyou, Wang, Qiumei, Lu, Lechen, Wang, Heng, Zhang, Guihong. 2023. PSMB4 Degrades the Porcine Reproductive and Respiratory Syndrome Virus Nsp1α Protein via the Autolysosome Pathway and Induces the Production of Type I Interferon. In Journal of virology, 97, e0026423. doi:10.1128/jvi.00264-23. https://pubmed.ncbi.nlm.nih.gov/36943051/
3. Yang, Chen, Yu, Pengyi, Yang, Fangfang, Wang, Hui, Zhang, Lei. 2021. PSMB4 inhibits cardiomyocyte apoptosis via activating NF-κB signaling pathway during myocardial ischemia/reperfusion injury. In Journal of molecular histology, 52, 693-703. doi:10.1007/s10735-021-09977-x. https://pubmed.ncbi.nlm.nih.gov/33954843/
4. Zhou, Dong-Mei, Liu, Jun, Liu, Fang, Chen, Bi-Liang, Hua, Wei. 2019. A novel FoxM1-PSMB4 axis contributes to proliferation and progression of cervical cancer. In Biochemical and biophysical research communications, 521, 746-752. doi:10.1016/j.bbrc.2019.10.183. https://pubmed.ncbi.nlm.nih.gov/31699366/
5. Liu, Rong, Lu, Shumin, Deng, Yan, Mao, Guoxin, Wang, Yingying. 2015. PSMB4 expression associates with epithelial ovarian cancer growth and poor prognosis. In Archives of gynecology and obstetrics, 293, 1297-307. doi:10.1007/s00404-015-3904-x. https://pubmed.ncbi.nlm.nih.gov/26439929/
6. Lin, Yi-Hsuan, Chen, Tzu-Min, Tsai, Yu-Ling, Chen, Ying, Wu, Sheng-Tang. 2024. The Reduction of PSMB4 in T24 and J82 Bladder Cancer Cells Inhibits the Angiogenesis and Migration of Endothelial Cells. In International journal of molecular sciences, 25, . doi:10.3390/ijms25105559. https://pubmed.ncbi.nlm.nih.gov/38791597/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen