C57BL/6JCya-Ptbp1em1flox/Cya
Common Name:
Ptbp1-flox
Product ID:
S-CKO-04504
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Ptbp1-flox
Strain ID
CKOCMP-19205-Ptbp1-B6J-VA
Gene Name
Product ID
S-CKO-04504
Gene Alias
HNRPI; PTB-1; PTB2; PTB3; PTB4; Ptb; pPTB
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ptbp1em1flox/Cya mice (Catalog S-CKO-04504) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000172282
NCBI RefSeq
NM_001077363
Target Region
Exon 3~8
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Ptbp1, also known as Polypyrimidine tract-binding protein 1, is a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) family. It plays a key role in alternative splicing of precursor mRNA and RNA metabolism, and is universally expressed in various tissues [2]. By binding to multiple downstream transcripts, it interferes with physiological and pathological processes such as tumor growth, body metabolism, cardiovascular homeostasis, and central nervous system damage [2].
In glioblastoma cells, knockdown of Ptbp1 promotes neural differentiation via the UNC5B receptor, suppressing cancer cell proliferation in vitro and in vivo, providing a potential approach for glioblastoma treatment [1]. In the context of glioma stem cells, PTBP1-K436 lactylation supports glioma progression and stem cell maintenance through enhancing PFKFB4-driven glycolysis, suggesting it could be a target for glioma therapies [3]. In senescent cells, inhibition of PTBP1 prevents the pro-tumorigenic effects of the senescence-associated secretory phenotype (SASP) without increasing tumorigenesis risk, indicating SASP inhibition as a therapy against inflammation-driven cancer [4].
In conclusion, Ptbp1 is essential in RNA processing and metabolism, influencing multiple physiological and pathological processes. Model-based research, especially through knockdown experiments in cell lines, has revealed its significant roles in cancer, such as in glioblastoma and glioma, highlighting its potential as a therapeutic target in these disease areas.
References:
1. Wang, Kankai, Pan, Sishi, Zhao, Peiqi, Zhuge, Qichuan, Yang, Jianjing. 2022. PTBP1 knockdown promotes neural differentiation of glioblastoma cells through UNC5B receptor. In Theranostics, 12, 3847-3861. doi:10.7150/thno.71100. https://pubmed.ncbi.nlm.nih.gov/35664063/
2. Yu, Qi, Wu, Tongtong, Xu, Wenhong, Li, Meiying, Chi, Guangfan. 2023. PTBP1 as a potential regulator of disease. In Molecular and cellular biochemistry, 479, 2875-2894. doi:10.1007/s11010-023-04905-x. https://pubmed.ncbi.nlm.nih.gov/38129625/
3. Zhou, Zijian, Yin, Xianyong, Sun, Hao, Wang, Shan, Xin, Tao. . PTBP1 Lactylation Promotes Glioma Stem Cell Maintenance through PFKFB4-Driven Glycolysis. In Cancer research, 85, 739-757. doi:10.1158/0008-5472.CAN-24-1412. https://pubmed.ncbi.nlm.nih.gov/39570804/
4. Georgilis, Athena, Klotz, Sabrina, Hanley, Christopher J, Zender, Lars, Gil, Jesús. . PTBP1-Mediated Alternative Splicing Regulates the Inflammatory Secretome and the Pro-tumorigenic Effects of Senescent Cells. In Cancer cell, 34, 85-102.e9. doi:10.1016/j.ccell.2018.06.007. https://pubmed.ncbi.nlm.nih.gov/29990503/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen