C57BL/6JCya-Sardhem1flox/Cya
Common Name:
Sardh-flox
Product ID:
S-CKO-04526
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Sardh-flox
Strain ID
CKOCMP-192166-Sardh-B6J-VA
Gene Name
Product ID
S-CKO-04526
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Sardhem1flox/Cya mice (Catalog S-CKO-04526) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000102886
NCBI RefSeq
NM_138665
Target Region
Exon 3
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Sardh, or sarcosine dehydrogenase, is involved in the metabolism of the glycine‑derivative sarcosine. It participates in pathways related to one-carbon metabolism and is linked to the purine cycle [1,3,4]. It has been found to be of biological importance in processes like muscle function regulation, sex pheromone biosynthesis, and is associated with tumorigenesis [1,2,3,4,5].
In Drosophila obesity models, muscle-specific knockdown of Sardh leads to muscle dysfunction, ectopic lipid accumulation, and loss of the benefits of time-restricted feeding, indicating its role in muscle function improvement through the purine cycle and AMPK signaling [1]. In sporadic colorectal cancer, SARDH was identified as a novel tumor suppressor gene. Its overexpression inhibited the proliferation, migration, and invasion of CRC cell lines, while depletion improved these processes. Also, in renal cell carcinoma, lower methylation of a locus in the SARDH gene was associated with aggressive tumor features, and SARDH methylation was a significant prognostic factor for recurrence-free survival [2,3].
In conclusion, Sardh plays essential roles in muscle function, one-carbon metabolism, and sex pheromone biosynthesis. Its role as a potential tumor suppressor in colorectal and renal cell carcinomas is also significant. Functional studies, especially through loss-of-function models like those in Drosophila and cell-line based experiments, have been crucial in revealing these functions, providing insights into disease mechanisms and potential treatment targets.
References:
1. Livelo, Christopher, Guo, Yiming, Abou Daya, Farah, Panda, Satchidananda, Melkani, Girish C. 2023. Time-restricted feeding promotes muscle function through purine cycle and AMPK signaling in Drosophila obesity models. In Nature communications, 14, 949. doi:10.1038/s41467-023-36474-4. https://pubmed.ncbi.nlm.nih.gov/36810287/
2. He, Hongjuan, Chen, Erfei, Lei, Lei, Dong, Jing, Yang, Jin. 2019. Alteration of the tumor suppressor SARDH in sporadic colorectal cancer: A functional and transcriptome profiling-based study. In Molecular carcinogenesis, 58, 957-966. doi:10.1002/mc.22984. https://pubmed.ncbi.nlm.nih.gov/30693981/
3. Mazdak, Mehrdad, Tezval, Hossein, Callauch, Janne Carmen, Kuczyk, Markus A, Serth, Jürgen. 2019. DNA methylation of sarcosine dehydrogenase (SARDH) loci as a prognosticator for renal cell carcinoma. In Oncology reports, 42, 2159-2168. doi:10.3892/or.2019.7305. https://pubmed.ncbi.nlm.nih.gov/31545450/
4. Green, Thomas, Chen, Xiaofei, Ryan, Stephen, Asch, Adam S, Ruiz-Echevarría, Maria J. 2013. TMEFF2 and SARDH cooperate to modulate one-carbon metabolism and invasion of prostate cancer cells. In The Prostate, 73, 1561-75. doi:10.1002/pros.22706. https://pubmed.ncbi.nlm.nih.gov/23824605/
5. Gui, Shiyu, Yuval, Boaz, Engl, Tobias, Lu, Yongyue, Cheng, Daifeng. 2023. Protein feeding mediates sex pheromone biosynthesis in an insect. In eLife, 12, . doi:10.7554/eLife.83469. https://pubmed.ncbi.nlm.nih.gov/36656757/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen