C57BL/6NCya-Ptger2em1flox/Cya
Common Name
Ptger2-flox
Product ID
S-CKO-04529
Backgroud
C57BL/6NCya
Strain ID
CKOCMP-19217-Ptger2-B6N-VA
When using this mouse strain in a publication, please cite “Ptger2-flox Mouse (Catalog S-CKO-04529) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Ptger2-flox
Strain ID
CKOCMP-19217-Ptger2-B6N-VA
Gene Name
Product ID
S-CKO-04529
Gene Alias
EP2, Ptgerep2
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 14
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000046891
NCBI RefSeq
NM_008964
Target Region
Exon 1
Size of Effective Region
~1.1 kb
Overview of Gene Research
Ptger2, short for prostaglandin E receptor 2, is a G protein-coupled receptor that binds prostaglandin E2 (PGE2). The PTGS2 (COX-2)-PTGER2 pathway is involved in inflammation and carcinogenesis [3]. PGE2-PTGER2 signaling is associated with various biological processes, such as cell proliferation, regulation of the cell cycle, and endometrial repair [2,4].
In mice, the combined knockout of PTGER2 and PTGER4 strongly enhanced the pathology induced by streptozotocin, indicating that the PTGS-2-PTGER2/4 signaling pathway has a protective role against streptozotocin-induced diabetes [6]. In fibrotic fibroblasts from humans and mice, DNA hypermethylation of PTGER2 leads to decreased expression levels of the receptor and PGE2 resistance [5]. In ovarian cancer, METTL3-mediated m6A modification enhances PTGER2 expression, which in turn promotes cancer stem cell properties, therapy resistance, and metastasis [1]. In colorectal cancer, PTGER2 overexpression is associated with microsatellite instability [3].
In conclusion, Ptger2 is crucial in multiple biological processes and disease conditions. Studies using gene knockout models in mice have revealed its protective role in diabetes-related toxicity, and its association with fibrosis, ovarian cancer, and colorectal cancer. These findings suggest that Ptger2 could be a potential therapeutic target in these diseases.
References：
1. Lin, Yi-Bin, Xu, Ben-Hua. . N6-methyladenosine Methyltransferase METTL3 Enhances PTGER2 Expression to Increase Ovarian Cancer Stemness and Chemoresistance. In Frontiers in bioscience (Landmark edition), 28, 199. doi:10.31083/j.fbl2809199. https://pubmed.ncbi.nlm.nih.gov/37796697/
2. Gao, Long, Liu, Bo, Mao, Wei, Wu, Xing, Cao, Jinshan. 2017. PTGER2 activation induces PTGS-2 and growth factor gene expression in endometrial epithelial cells of cattle. In Animal reproduction science, 187, 54-63. doi:10.1016/j.anireprosci.2017.10.005. https://pubmed.ncbi.nlm.nih.gov/29033117/
3. Baba, Yoshifumi, Nosho, Katsuhiko, Shima, Kaori, Fuchs, Charles S, Ogino, Shuji. 2010. PTGER2 overexpression in colorectal cancer is associated with microsatellite instability, independent of CpG island methylator phenotype. In Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 19, 822-31. doi:10.1158/1055-9965.EPI-09-1154. https://pubmed.ncbi.nlm.nih.gov/20200425/
4. Liu, Bo, Fu, Changqi, Cao, Jinshan, Zhao, Jiamin, Feng, Shuang. 2021. Proliferation of bovine endometrial epithelial cells is promoted by prostaglandin E2-PTGER2 signaling through cell cycle regulation. In Prostaglandins, leukotrienes, and essential fatty acids, 174, 102362. doi:10.1016/j.plefa.2021.102362. https://pubmed.ncbi.nlm.nih.gov/34740034/
5. Huang, Steven K, Fisher, Aaron S, Scruggs, Anne M, Richardson, Bruce C, Peters-Golden, Marc. 2010. Hypermethylation of PTGER2 confers prostaglandin E2 resistance in fibrotic fibroblasts from humans and mice. In The American journal of pathology, 177, 2245-55. doi:10.2353/ajpath.2010.100446. https://pubmed.ncbi.nlm.nih.gov/20889571/
6. Vennemann, Antje, Gerstner, Anemone, Kern, Niklas, Maruyama, Takayuki, Nüsing, Rolf M. 2012. PTGS-2-PTGER2/4 signaling pathway partially protects from diabetogenic toxicity of streptozotocin in mice. In Diabetes, 61, 1879-87. doi:10.2337/db11-1396. https://pubmed.ncbi.nlm.nih.gov/22522619/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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