C57BL/6JCya-Luc7l2em1flox/Cya
Common Name
Luc7l2-flox
Product ID
S-CKO-04546
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-192196-Luc7l2-B6J-VA
When using this mouse strain in a publication, please cite “Luc7l2-flox Mouse (Catalog S-CKO-04546) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Luc7l2-flox
Strain ID
CKOCMP-192196-Luc7l2-B6J-VA
Gene Name
Product ID
S-CKO-04546
Gene Alias
CGI59, Cgi74, 4930471C18Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 6
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000161538
NCBI RefSeq
NM_001170849
Target Region
Exon 5~7
Size of Effective Region
~3.1 kb
Overview of Gene Research
Luc7l2, a paralog of the yeast U1 small nuclear RNA (snRNA)-associated splicing factor Luc7p, is involved in pre-mRNA splicing regulation. It is associated with multiple biological pathways such as energy metabolism, DNA damage repair, and innate immune response, and is of great biological importance in various physiological and pathological processes [6].
In glioblastoma, histone H3K9 lactylation activates LUC7L2 transcription. LUC7L2 then mediates intron 7 retention of MLH1, reducing MLH1 expression, inhibiting mismatch repair, and conferring temozolomide resistance [1]. In liver cancer cells, LUC7L2 promotes proliferation by enhancing DNA damage repair via RRAS [3]. In nasopharyngeal carcinoma, LUC7L2 promotes radioresistance through autophagy, and high LUC7L2 expression is associated with shorter survival in patients [5]. Loss of LUC7L2 shifts energy metabolism from glycolysis to OXPHOS by multiple splicing-related mechanisms [2]. Also, LUC7L2 negatively regulates the innate antiviral response by mediating MITA/STING intron retention [4].
In conclusion, Luc7l2 is crucial in pre-mRNA splicing and significantly impacts multiple disease-related processes such as drug resistance in glioblastoma, proliferation in liver cancer, radioresistance in nasopharyngeal carcinoma, energy metabolism regulation, and innate antiviral response. Studies, including those potentially using KO/CKO mouse models (although not explicitly detailed in all references), have enhanced our understanding of its role in these disease areas, providing potential therapeutic targets.
References：
1. Yue, Qu, Wang, Zhao, Shen, Yixiong, Liu, Boyang, Guo, Hongbo. 2024. Histone H3K9 Lactylation Confers Temozolomide Resistance in Glioblastoma via LUC7L2-Mediated MLH1 Intron Retention. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 11, e2309290. doi:10.1002/advs.202309290. https://pubmed.ncbi.nlm.nih.gov/38477507/
2. Jourdain, Alexis A, Begg, Bridget E, Mick, Eran, Burge, Christopher B, Mootha, Vamsi K. 2021. Loss of LUC7L2 and U1 snRNP subunits shifts energy metabolism from glycolysis to OXPHOS. In Molecular cell, 81, 1905-1919.e12. doi:10.1016/j.molcel.2021.02.033. https://pubmed.ncbi.nlm.nih.gov/33852893/
3. Liu, Xinlei, Xie, Sijie, Jiang, Xiaoxue, Li, Shujie, Lu, Dongdong. 2024. LUC7L2 accelerates the growth of liver cancer cells by enhancing DNA damage repair via RRAS. In Cells & development, 180, 203976. doi:10.1016/j.cdev.2024.203976. https://pubmed.ncbi.nlm.nih.gov/39571735/
4. Li, Chen, Feng, Lu, Luo, Wei-Wei, Li, Mi, Shu, Hong-Bing. 2021. The RNA-binding protein LUC7L2 mediates MITA/STING intron retention to negatively regulate innate antiviral response. In Cell discovery, 7, 46. doi:10.1038/s41421-021-00277-y. https://pubmed.ncbi.nlm.nih.gov/34155193/
5. Shen, Lin, Li, Chao, Chen, Fang, Li, Zhanzhan, Li, Na. 2021. CRISPR/Cas9 genome-wide screening identifies LUC7L2 that promotes radioresistance via autophagy in nasopharyngeal carcinoma cells. In Cell death discovery, 7, 392. doi:10.1038/s41420-021-00783-8. https://pubmed.ncbi.nlm.nih.gov/34907164/
6. Daniels, Noah J, Hershberger, Courtney E, Gu, Xiaorong, Maciejewski, Jaroslaw P, Padgett, Richard A. . Functional analyses of human LUC7-like proteins involved in splicing regulation and myeloid neoplasms. In Cell reports, 35, 108989. doi:10.1016/j.celrep.2021.108989. https://pubmed.ncbi.nlm.nih.gov/33852859/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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