C57BL/6JCya-Ptgs2em1flox/Cya
Common Name:
Ptgs2-flox
Product ID:
S-CKO-04560
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ptgs2-flox
Strain ID
CKOCMP-19225-Ptgs2-B6J-VA
Gene Name
Product ID
S-CKO-04560
Gene Alias
COX2; Cox-2; PES-2; PGHS-2; PHS II; PHS-2; Pghs2; TIS10; gripghs
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ptgs2em1flox/Cya mice (Catalog S-CKO-04560) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000035065
NCBI RefSeq
NM_011198
Target Region
Exon 2~5
Size of Effective Region
~2.3 kb
Detailed Document
Overview of Gene Research
Ptgs2, also known as Prostaglandin-endoperoxide synthase 2 or Cyclooxygenase 2 (COX2), is the rate-limiting enzyme in the production of prostaglandins (PGs) [6]. It is involved in the PTGS2/COX2-PGE2 signaling cascade, which is associated with various physiological and pathophysiological processes, such as constriction or dilation of blood vessels, inflammation, pain, and fever [1].
In the context of multiple diseases, studies have revealed its significance. In atherosclerosis, PTGS2 was upregulated in advanced stages, and it might be the hub gene with its expression positively associated with the disease severity [2]. In type 1 diabetes mellitus, the PTGS2/COX2-PGE2 signaling cascade has controversial effects on pancreatic islet cells, being both a cause and consequence of inflammation [1]. In chronic spontaneous urticaria, PTGS2 may be a potential immune regulator, and its inhibition could be a new treatment perspective [5]. In colorectal cancer, PTGS2-positive patients had an increased risk of tumor recurrence and poorer cancer-specific survival [4]. Also, RUNX1-induced upregulation of PTGS2 promotes CRC cell growth, migration, and invasion [9]. In nasopharyngeal carcinoma, miR-26a-5p suppresses the progression by inhibiting PTGS2 expression [10]. In intervertebral disc degeneration, the ERBB2-PTGS2 axis is involved in regulating nucleus pulposus cell senescence [8]. In pulmonary fibrosis, ADAM17/PTGS2 promotes the disease by regulating ferroptosis in fibroblasts [7]. Endothelial ROBO4 can suppress PTGS2/COX-2 expression and associated inflammatory diseases [3].
In conclusion, Ptgs2 is a crucial gene in the production of prostaglandins and is involved in multiple disease-related processes. Studies, including those using potential gene-knockout models in the context of various diseases like atherosclerosis, diabetes, cancer, and inflammatory skin and lung diseases, have helped in understanding its role, providing insights into possible therapeutic strategies targeting Ptgs2 in these disease areas.
References:
1. Martín-Vázquez, Eugenia, Cobo-Vuilleumier, Nadia, López-Noriega, Livia, Lorenzo, Petra I, Gauthier, Benoit R. 2023. The PTGS2/COX2-PGE2 signaling cascade in inflammation: Pro or anti? A case study with type 1 diabetes mellitus. In International journal of biological sciences, 19, 4157-4165. doi:10.7150/ijbs.86492. https://pubmed.ncbi.nlm.nih.gov/37705740/
2. Zhou, Yaqing, Zhou, Hanxiao, Hua, Lei, He, Shu, Jia, Enzhi. 2021. Verification of ferroptosis and pyroptosis and identification of PTGS2 as the hub gene in human coronary artery atherosclerosis. In Free radical biology & medicine, 171, 55-68. doi:10.1016/j.freeradbiomed.2021.05.009. https://pubmed.ncbi.nlm.nih.gov/33974977/
3. Tanaka, Masato, Shirakura, Keisuke, Takayama, Yui, Takayama, Kazuo, Okada, Yoshiaki. 2024. Endothelial ROBO4 suppresses PTGS2/COX-2 expression and inflammatory diseases. In Communications biology, 7, 599. doi:10.1038/s42003-024-06317-z. https://pubmed.ncbi.nlm.nih.gov/38762541/
4. Kunzmann, Andrew T, Murray, Liam J, Cardwell, Chris R, McMenamin, Una C, Cantwell, Marie M. 2013. PTGS2 (Cyclooxygenase-2) expression and survival among colorectal cancer patients: a systematic review. In Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 22, 1490-7. doi:10.1158/1055-9965.EPI-13-0263. https://pubmed.ncbi.nlm.nih.gov/23810915/
5. Chen, Yihui, Jian, Xingxing, Zhu, Lei, Xiong, Feng, Li, Jie. 2024. PTGS2: A potential immune regulator and therapeutic target for chronic spontaneous urticaria. In Life sciences, 344, 122582. doi:10.1016/j.lfs.2024.122582. https://pubmed.ncbi.nlm.nih.gov/38514006/
6. Anamthathmakula, Prashanth, Winuthayanon, Wipawee. . Prostaglandin-Endoperoxide Synthase 2 (PTGS2) in the Oviduct: Roles in Fertilization and Early Embryo Development. In Endocrinology, 162, . doi:10.1210/endocr/bqab025. https://pubmed.ncbi.nlm.nih.gov/33539521/
7. Yan, Suyan, Zhao, Yaqi, Xu, Wei, Ma, Zhenzhen, Yang, Qingrui. . ADAM17/PTGS2 Facilitates Pulmonary Fibrosis by Regulating Ferroptosis. In Journal of cellular and molecular medicine, 29, e70466. doi:10.1111/jcmm.70466. https://pubmed.ncbi.nlm.nih.gov/40077919/
8. Li, Zhao-Cheng, An, Fu. 2023. ERBB2-PTGS2 axis promotes intervertebral disc degeneration by regulating senescence of nucleus pulposus cells. In BMC musculoskeletal disorders, 24, 504. doi:10.1186/s12891-023-06625-1. https://pubmed.ncbi.nlm.nih.gov/37340393/
9. Zheng, Weiwei, Guo, Yingchang, Kahar, Aihemaiti, Zhang, Chao, Zhu, Yuanzeng. 2024. RUNX1-induced upregulation of PTGS2 enhances cell growth, migration and invasion in colorectal cancer cells. In Scientific reports, 14, 11670. doi:10.1038/s41598-024-60296-z. https://pubmed.ncbi.nlm.nih.gov/38778047/
10. Cai, Binlin, Qu, Xiu, Kan, Dan, Luo, Yi. 2022. miR-26a-5p suppresses nasopharyngeal carcinoma progression by inhibiting PTGS2 expression. In Cell cycle (Georgetown, Tex.), 21, 618-629. doi:10.1080/15384101.2022.2030168. https://pubmed.ncbi.nlm.nih.gov/35073820/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen