C57BL/6JCya-Ptmaem1flox/Cya
Common Name:
Ptma-flox
Product ID:
S-CKO-04569
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ptma-flox
Strain ID
CKOCMP-19231-Ptma-B6J-VA
Gene Name
Product ID
S-CKO-04569
Gene Alias
Thym
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ptmaem1flox/Cya mice (Catalog S-CKO-04569) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000045897
NCBI RefSeq
NM_008972
Target Region
Exon 2~5
Size of Effective Region
~2.9 kb
Detailed Document
Overview of Gene Research
PTMA, short for prothymosin alpha, is a protein-coding gene with significant biological functions. It is involved in various cellular processes such as cell proliferation, differentiation, and apoptosis. PTMA has been shown to play a role in regulating mitochondrial oxidative phosphorylation through binding to HMGB1, thus affecting the malignant progression of esophageal squamous cell carcinoma (ESCC) [1]. It also appears to be related to fibrosis-related processes, as it is positively correlated with TGFβ1 and SMAD4 in oral submucous fibrosis, and its knockdown or overexpression can reverse or enhance TGFβ1-induced fibrosis in primary human oral submucous fibroblasts respectively [2].
In studies related to diseases, in ESCC, PTMA expression is abnormally elevated, and its inhibition can decrease ESCC cell activity and increase apoptosis, potentially through inhibiting mitochondrial oxidative phosphorylation by binding to HMGB1 [1]. In oral submucous fibrosis, PTMA is a newly identified autoantigen, and targeting it might be a promising treatment strategy [2]. In chronic apical periodontitis, PTMA upregulation moderates the inflammatory response and prevents macrophage osteoclastogenesis, providing a basis for targeted therapeutic strategies [3]. Also, in the context of HIV-1 infection, PTMA has been identified as a host factor that restricts HIV-1 transcription and infectious virus production in vivo [4].
In conclusion, PTMA plays crucial roles in multiple biological processes and disease conditions. Its functions in cancer development, fibrosis, and immune-related diseases have been revealed through various studies. Understanding PTMA provides insights into the mechanisms of these diseases and may offer potential targets for therapeutic interventions.
References:
1. Chen, Shaogeng, He, Rongqi, Lin, Xianzuan, Xu, Rongyu, Kang, Mingqiang. 2023. PTMA binds to HMGB1 to regulate mitochondrial oxidative phosphorylation and thus affect the malignant progression of esophageal squamous cell carcinoma. In Journal of thoracic disease, 15, 1302-1318. doi:10.21037/jtd-23-143. https://pubmed.ncbi.nlm.nih.gov/37065565/
2. Wang, Jie, You, Jialing, Wang, Lekai, Jia, Lina, Jiang, Canhua. 2017. PTMA, a new identified autoantigen for oral submucous fibrosis, regulates oral submucous fibroblast proliferation and extracellular matrix. In Oncotarget, 8, 74806-74819. doi:10.18632/oncotarget.20419. https://pubmed.ncbi.nlm.nih.gov/29088825/
3. Gong, Qimei, Lv, Xiaomin, Liao, Chenxi, Huang, Yihua, Tong, Zhongchun. 2024. Single-cell RNA sequencing combined with proteomics of infected macrophages reveals prothymosin-α as a target for treatment of apical periodontitis. In Journal of advanced research, 66, 349-361. doi:10.1016/j.jare.2024.01.018. https://pubmed.ncbi.nlm.nih.gov/38237771/
4. Geretz, Aviva, Ehrenberg, Philip K, Clifford, Robert J, Kirchhoff, Frank, Thomas, Rasmi. 2023. Single-cell transcriptomics identifies prothymosin α restriction of HIV-1 in vivo. In Science translational medicine, 15, eadg0873. doi:10.1126/scitranslmed.adg0873. https://pubmed.ncbi.nlm.nih.gov/37531416/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen