C57BL/6JCya-Ptpn22em1flox/Cya
Common Name:
Ptpn22-flox
Product ID:
S-CKO-04585
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ptpn22-flox
Strain ID
CKOCMP-19260-Ptpn22-B6J-VA
Gene Name
Product ID
S-CKO-04585
Gene Alias
70zpep; PEP; Ptpn8
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ptpn22em1flox/Cya mice (Catalog S-CKO-04585) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029433
NCBI RefSeq
NM_008979
Target Region
Exon 2~8
Size of Effective Region
~3.6 kb
Detailed Document
Overview of Gene Research
Ptpn22, short for Protein tyrosine phosphatase nonreceptor type 22, is a protein tyrosine phosphatase that significantly impacts immune-related processes. It negatively regulates T-cell signaling, playing a key role in immune homeostasis [2,3,5,6]. Genetic models, such as knockout mouse models, are valuable for studying its functions.
Using PTPN22-/-mice, research shows that PTPN22 deficiency shortens tail-bleeding time and accelerates arterial thrombus formation without affecting venous thrombosis and certain coagulation factors. PTPN22-deficient platelets display enhanced functions like aggregation, granule secretion, and calcium mobilization [1]. Also, a genetic variant (R620W, rs2476601) in PTPN22 is associated with increased risk for multiple autoimmune diseases. Gene-edited T cells with the risk variant or knockout of PTPN22 show enhanced activation and signaling, especially in T cells with lower avidity TCRs [4].
In conclusion, Ptpn22 is crucial for regulating immune-related functions. The use of Ptpn22 KO mouse models has revealed its role in arterial thrombosis and autoimmune diseases, highlighting its potential as a target for treating thrombotic or autoimmune conditions [1,4].
References：
1. Wang, Xiamin, Wei, Guangyu, Ding, Yangyang, Xu, Kailin, Qiao, Jianlin. . Protein tyrosine phosphatase PTPN22 negatively modulates platelet function and thrombus formation. In Blood, 140, 1038-1051. doi:10.1182/blood.2022015554. https://pubmed.ncbi.nlm.nih.gov/35767715/
2. Jassim, Brenson A, Lin, Jianping, Zhang, Zhong-Yin. 2022. PTPN22: structure, function, and developments in inhibitor discovery with applications for immunotherapy. In Expert opinion on drug discovery, 17, 825-837. doi:10.1080/17460441.2022.2084607. https://pubmed.ncbi.nlm.nih.gov/35637605/
3. Mustelin, Tomas, Bottini, Nunzio, Stanford, Stephanie M. 2019. The Contribution of PTPN22 to Rheumatic Disease. In Arthritis & rheumatology (Hoboken, N.J.), 71, 486-495. doi:10.1002/art.40790. https://pubmed.ncbi.nlm.nih.gov/30507064/
4. Anderson, Warren, Barahmand-Pour-Whitman, Fariba, Linsley, Peter S, Buckner, Jane H, Rawlings, David J. 2023. PTPN22 R620W gene editing in T cells enhances low-avidity TCR responses. In eLife, 12, . doi:10.7554/eLife.81577. https://pubmed.ncbi.nlm.nih.gov/36961507/
5. Stanford, Stephanie M, Bottini, Nunzio. 2014. PTPN22: the archetypal non-HLA autoimmunity gene. In Nature reviews. Rheumatology, 10, 602-11. doi:10.1038/nrrheum.2014.109. https://pubmed.ncbi.nlm.nih.gov/25003765/
6. Armitage, Lucas H, Wallet, Mark A, Mathews, Clayton E. 2021. Influence of PTPN22 Allotypes on Innate and Adaptive Immune Function in Health and Disease. In Frontiers in immunology, 12, 636618. doi:10.3389/fimmu.2021.636618. https://pubmed.ncbi.nlm.nih.gov/33717184/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen