C57BL/6JCya-Sirpaem1flox/Cya
Common Name:
Sirpa-flox
Product ID:
S-CKO-04586
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Sirpa-flox
Strain ID
CKOCMP-19261-Sirpa-B6J-VA
Gene Name
Product ID
S-CKO-04586
Gene Alias
Bit; CD172a; Idd13.2; P84; Ptpns1; SHP-1; SHPS-1; SIRP
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Sirpaem1flox/Cya mice (Catalog S-CKO-04586) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000161620
NCBI RefSeq
NM_001291020
Target Region
Exon 4~6
Size of Effective Region
~3.6 kb
Detailed Document
Overview of Gene Research
Sirpa, Signal-regulatory protein alpha, is a transmembrane protein. Its ligand is CD47, and their interaction is a key "don't eat me" signal pathway. When CD47 on target cells binds to Sirpa on macrophages and dendritic cells, it suppresses phagocytosis, playing a crucial role in immune regulation, especially in the context of cancer immune escape [2,4,5,6,7]. Genetic models, such as gene knockout (KO) mouse models, are valuable for studying its functions.
In melanoma, tumor-intrinsic Sirpa enhances antitumor immunity and sensitivity to checkpoint inhibition immunotherapy. Mice bearing Sirpa-deficient melanoma tumors show no response to anti-PD-L1 treatment, indicating that Sirpa is essential for immunotherapy efficacy in melanoma [1]. In osteosarcoma, knockdown of Sirpa impairs cell migration by decreasing SP1 stability and arginine uptake, suggesting that Sirpa promotes metastasis in osteosarcoma [3].
In conclusion, Sirpa plays a complex role in the tumor ecosystem. Through KO mouse models, its significance in melanoma immunotherapy and osteosarcoma metastasis has been revealed. Understanding Sirpa's functions can provide potential therapeutic targets for cancer treatment.
References：
1. Zhou, Zhicheng, Chen, Mei-Ju May, Luo, Yikai, Lu, Yiling, Liang, Han. 2022. Tumor-intrinsic SIRPA promotes sensitivity to checkpoint inhibition immunotherapy in melanoma. In Cancer cell, 40, 1324-1340.e8. doi:10.1016/j.ccell.2022.10.012. https://pubmed.ncbi.nlm.nih.gov/36332624/
2. Morrissey, Meghan A, Kern, Nadja, Vale, Ronald D. 2020. CD47 Ligation Repositions the Inhibitory Receptor SIRPA to Suppress Integrin Activation and Phagocytosis. In Immunity, 53, 290-302.e6. doi:10.1016/j.immuni.2020.07.008. https://pubmed.ncbi.nlm.nih.gov/32768386/
3. Wang, Peng, Song, Yihui, Li, Hongyu, Ma, Mengjun, Shen, Huiyong. 2023. SIRPA enhances osteosarcoma metastasis by stabilizing SP1 and promoting SLC7A3-mediated arginine uptake. In Cancer letters, 576, 216412. doi:10.1016/j.canlet.2023.216412. https://pubmed.ncbi.nlm.nih.gov/37769797/
4. Willingham, Stephen B, Volkmer, Jens-Peter, Gentles, Andrew J, Clarke, Michael F, Weissman, Irving L. 2012. The CD47-signal regulatory protein alpha (SIRPa) interaction is a therapeutic target for human solid tumors. In Proceedings of the National Academy of Sciences of the United States of America, 109, 6662-7. doi:10.1073/pnas.1121623109. https://pubmed.ncbi.nlm.nih.gov/22451913/
5. Logtenberg, Meike E W, Scheeren, Ferenc A, Schumacher, Ton N. . The CD47-SIRPα Immune Checkpoint. In Immunity, 52, 742-752. doi:10.1016/j.immuni.2020.04.011. https://pubmed.ncbi.nlm.nih.gov/32433947/
6. Tang, Xao X, Shimada, Hiroyuki, Ikegaki, Naohiko. 2024. A Perspective on the CD47-SIRPA Axis in High-Risk Neuroblastoma. In Current oncology (Toronto, Ont.), 31, 3212-3226. doi:10.3390/curroncol31060243. https://pubmed.ncbi.nlm.nih.gov/38920727/
7. Jia, Xiao, Yan, Bingjun, Tian, Xiaoqing, Shi, Juanjuan, Hou, Yongzhong. 2021. CD47/SIRPα pathway mediates cancer immune escape and immunotherapy. In International journal of biological sciences, 17, 3281-3287. doi:10.7150/ijbs.60782. https://pubmed.ncbi.nlm.nih.gov/34512146/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen