C57BL/6JCya-Pvt1em1flox/Cya
Common Name:
Pvt1-flox
Product ID:
S-CKO-04626
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Pvt1-flox
Strain ID
CKOCMP-19296-Pvt1-B6J-VA
Gene Name
Product ID
S-CKO-04626
Gene Alias
Ayu21-84Imeg; Gt(pU21)84Imeg; Mis-1; Mlvi-1; Pvt-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pvt1em1flox/Cya mice (Catalog S-CKO-04626) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000180432
NCBI RefSeq
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Target Region
Exon 6~8
Size of Effective Region
~3.8 kb
Detailed Document
Overview of Gene Research
Pvt1, also known as plasmacytoma variant translocation 1, is a long non-coding RNA. It has oncogenic properties and is involved in regulating the proliferation and growth of many cancers. Pvt1 exerts its functions, in part, through microRNAs (miRNAs), and is associated with several cancer-related pathways such as TGF-β, Wnt/β-catenin, PI3K/AKT, and mTOR pathways [1,4].
In pancreatic cancer, gain-and loss-of-function assays in vitro and in vivo showed that Pvt1 impairs the sensitivity to gemcitabine by up-regulating Pygo2 and ATG14 through sponging miR-619-5p, thus regulating Wnt/β-catenin signaling and autophagic activity [2].
In liver cancer, depletion of lncPvt1 accelerated ferroptosis of liver cancer cells, and ketamine-induced cell growth suppression and ferroptosis were related to the regulation of the lncPvt1/miR-214-3p/GPX4 axis [3].
In gastric cancer, depletion of Pvt1 promoted the expression of miR-16 and suppressed CCND1 expression, inhibiting the viability, invasion and cell cycle progression of GC cells [5].
In conclusion, Pvt1 plays a crucial role in cancer progression, especially in pancreatic, liver and gastric cancers. Loss-of-function experiments in these cancer models have revealed its oncogenic role, mainly through miRNA-mediated mechanisms and regulation of related signaling pathways, providing potential therapeutic targets for cancer treatment.
References：
1. Derderian, Camille, Orunmuyi, Akintunde T, Olapade-Olaopa, E Oluwabunmi, Ogunwobi, Olorunseun O. 2019. PVT1 Signaling Is a Mediator of Cancer Progression. In Frontiers in oncology, 9, 502. doi:10.3389/fonc.2019.00502. https://pubmed.ncbi.nlm.nih.gov/31249809/
2. Zhou, Cefan, Yi, Changhua, Yi, Yongxiang, Chen, Xing-Zhen, Tang, Jingfeng. 2020. LncRNA PVT1 promotes gemcitabine resistance of pancreatic cancer via activating Wnt/β-catenin and autophagy pathway through modulating the miR-619-5p/Pygo2 and miR-619-5p/ATG14 axes. In Molecular cancer, 19, 118. doi:10.1186/s12943-020-01237-y. https://pubmed.ncbi.nlm.nih.gov/32727463/
3. He, Guan-Nan, Bao, Na-Ren, Wang, Shuang, Zhang, Tian-Hao, Chen, Feng-Shou. 2021. Ketamine Induces Ferroptosis of Liver Cancer Cells by Targeting lncRNA PVT1/miR-214-3p/GPX4. In Drug design, development and therapy, 15, 3965-3978. doi:10.2147/DDDT.S332847. https://pubmed.ncbi.nlm.nih.gov/34566408/
4. Ghafouri-Fard, Soudeh, Omrani, Mir Davood, Taheri, Mohammad. 2019. Long noncoding RNA PVT1: A highly dysregulated gene in malignancy. In Journal of cellular physiology, 235, 818-835. doi:10.1002/jcp.29060. https://pubmed.ncbi.nlm.nih.gov/31297833/
5. Lv, Haidong, Zhou, Dixia, Liu, Guoqing. 2023. PVT1/miR-16/CCND1 axis regulates gastric cancer progression. In Open medicine (Warsaw, Poland), 18, 20220550. doi:10.1515/med-2022-0550. https://pubmed.ncbi.nlm.nih.gov/36760720/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen