C57BL/6JCya-Zfp3em1flox/Cya
Common Name:
Zfp3-flox
Product ID:
S-CKO-04638
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Zfp3-flox
Strain ID
CKOCMP-193043-Zfp3-B6J-VA
Gene Name
Product ID
S-CKO-04638
Gene Alias
B230309J24; Fnp-1; Zfp-3
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Zfp3em1flox/Cya mice (Catalog S-CKO-04638) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000060444
NCBI RefSeq
NM_177565
Target Region
Exon 2
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
Zfp3, a C2H2-type zinc finger protein gene, is involved in multiple biological processes. It plays a role in stress responses such as salt and osmotic stress, and is also associated with plant development including vegetative growth and root hair development. In plants, it may regulate related pathways by affecting gene expressions [1,2].
In Arabidopsis, studies have shown that Zfp3 has a significant impact on stress tolerance. The constitutive expression of Zfp3 enhanced plant tolerance to salt and osmotic stress, while the zfp3 mutant plants had reduced tolerance. Also, gain-and loss-of-function studies demonstrated its role in proline accumulation, chlorophyll content, and the induction of stress-related genes like KIN1, RD22, RD29B, and AtP5CS1 [1]. In terms of plant development, Zfp3 overexpression reduced plant growth, limited cell expansion in leaves, and affected root hair development. RNAseq and chromatin immunoprecipitation experiments identified Zfp3-controlled gene sets related to cell wall biogenesis, cell differentiation, and root hair formation [2].
In conclusion, Zfp3 is crucial for plants in adapting to stress and in proper vegetative development. The functional studies using mutant plants have revealed its role in regulating key physiological processes, which contributes to our understanding of plant stress-response mechanisms and development regulation.
References:
1. Zhang, Aidong, Liu, Dongdong, Hua, Changmei, Ali, Imran, Gan, Yinbo. 2016. The Arabidopsis Gene zinc finger protein 3(ZFP3) Is Involved in Salt Stress and Osmotic Stress Response. In PloS one, 11, e0168367. doi:10.1371/journal.pone.0168367. https://pubmed.ncbi.nlm.nih.gov/27977750/
2. Benyó, Dániel, Bató, Emese, Faragó, Dóra, Nagy, István, Szabados, László. 2024. The zinc finger protein 3 of Arabidopsis thaliana regulates vegetative growth and root hair development. In Frontiers in plant science, 14, 1221519. doi:10.3389/fpls.2023.1221519. https://pubmed.ncbi.nlm.nih.gov/38250442/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen